scholarly journals Characterization of stromal vascular fraction and adipose stem cells from subcutaneous, preperitoneal and visceral morbidly obese human adipose tissue depots

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0174115 ◽  
Author(s):  
Karina Ribeiro Silva ◽  
Isis Côrtes ◽  
Sally Liechocki ◽  
João Regis Ivar Carneiro ◽  
Antônio Augusto Peixoto Souza ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Human Adipose Tissue ◽  
Adipose Stem Cells ◽  
Morbidly Obese ◽  
Adipose Tissue Depots ◽  
Obese Human

Obesity-associated mouse adipose stem cell secretion of monocyte chemotactic protein-1

2007 ◽  
Vol 293 (5) ◽  
pp. E1153-E1158 ◽  
Author(s):  
Hui Ren Zhou ◽  
Eun-Kyoung Kim ◽  
Hyojung Kim ◽  
Kate J. Claycombe
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ex Vivo ◽  
Stromal Vascular Fraction ◽  
Tissue Cell ◽  
Mrna Levels ◽  
Cell Secretion ◽  
Monocyte Chemotactic Protein ◽  
Adipose Tissue Depots ◽  
Chemotactic Protein

Studies showed that monocyte chemotactic protein-1 (MCP-1) concentrations are increased in obesity. In our current study, we demonstrate that plasma MCP-1 level in leptin-deficient ob/ob mice is significantly higher than in lean mice. Furthermore, we determined that basal adipose tissue MCP-1 mRNA levels are significantly higher in ob/ob mice compared with lean mice. To determine the mechanisms underlying obesity-associated increases in plasma and adipose tissue MCP-1 levels, we determined adipose tissue cell type sources of MCP-1 production. Our data show that adipose tissue stem cells (CD34+), macrophages (F4/80+), and stromal vascular fraction (SVF) cells express significantly higher levels of MCP-1 compared with adipocytes under both basal and lipopolysaccharide (LPS)-stimulated conditions. Furthermore, basal and LPS-induced MCP-1 secretion levels were the same for both adipose F4/80+ and CD34+ cells, whereas adipose CD34+ cells have twofold higher cell numbers (30% of total SVF cells) compared with F4/80+ macrophages (15%). Our data also show that CD34+ cells from visceral adipose tissue depots secrete significantly higher levels of MCP-1 ex vivo when compared with CD34+ cells from subcutaneous adipose tissue depots. Taken together, our data suggest that adipose CD34+ stem cells may play an important role in obesity-associated increases in plasma MCP-1 levels.

scholarly journals Chondrocytes Cocultured with Stromal Vascular Fraction of Adipose Tissue Present More Intense Chondrogenic Characteristics Than with Adipose Stem Cells

2016 ◽  
Vol 22 (3-4) ◽  
pp. 336-348 ◽  
Author(s):  
Ling Wu ◽  
Henk-Jan Prins ◽  
Jeroen Leijten ◽  
Marco N. Helder ◽  
Denis Evseenko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Adipose Stem Cells

Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties

2016 ◽  
Vol 11 (9) ◽  
pp. 2490-2502 ◽  
Author(s):  
Anne Therese Lauvrud ◽  
Peyman Kelk ◽  
Mikael Wiberg ◽  
Paul J. Kingham
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue

scholarly journals Generation and characterization of two iPSC lines from human adipose tissue-derived stem cells of healthy donors

2020 ◽  
Vol 48 ◽  
pp. 101973
Author(s):  
Xiang-Bin Zhou ◽  
Hong Li ◽  
Fei Li ◽  
Xian-Kui Song ◽  
Tun Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Healthy Donors

1762-P: Insulin Receptor Isoforms in Human Adipose Tissue and Adipose Stem Cells

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1762-P
Author(s):  
VALENTINA ANNAMARIA GENCHI ◽  
ANGELO CIGNARELLI ◽  
SEBASTIO PERRINI ◽  
STEFANIA PORRO ◽  
CRISTINA CACCIOPPOLI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Insulin Receptor ◽  
Human Adipose Tissue ◽  
Adipose Stem Cells ◽  
Receptor Isoforms ◽  
Insulin Receptor Isoforms

scholarly journals Characterization of Crystalline Phase of TiO2 Nanocrystals, Cytotoxicity and Cell Internalization Analysis on Human Adipose Tissue-Derived Mesenchymal Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4071
Author(s):  
Cristiane Angélico Duarte ◽  
Luiz Ricardo Goulart ◽  
Letícia de Souza Castro Filice ◽  
Isabela Lemos de Lima ◽  
Esther Campos-Fernández ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cellular Localization ◽  
Human Adipose Tissue ◽  
Rutile Phase ◽  
Tio2 Nanocrystals ◽  
Cell Internalization ◽  
The Impact

Titanium dioxide (TiO2) is manufactured worldwide as crystalline and amorphous forms for multiple applications, including tissue engineering, but our study proposes analyzing the impact of crystalline phases of TiO2 on Mesenchymal Stem Cells (MSCs). Several studies have already described the regenerative potential of MSCs and TiO2 has been used for bone regeneration. In this study, polydispersity index and sizes of TiO2 nanocrystals (NCs) were determined. Adipose tissue-derived Mesenchymal Stem Cells (AT-MSCs) were isolated and characterized in order to evaluate cellular viability and the internalization of nanocrystals (NCs). All of the assays were performed using the TiO2 NCs with 100% anatase (A), 91.6% anatase/9.4% rutile (AR), 64.6% rutile/35.4% anatase (RA), and 84.0% rutile/16% brookite (RB), submitted to several concentrations in 24-h treatments. Cellular localization of TiO2 NCs in the AT-MSCs was resolved by europium-doped NCs. Viability was significantly improved under the predominance of the rutile phase in NCs with localization restricted at the cytoplasm, suggesting that AR and RA NCs are not genotoxic and can be associated with most cellular activities and metabolic pathways, including glycolysis and cell division.

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