Characterization of Crystalline Phase of TiO2 Nanocrystals, Cytotoxicity and Cell Internalization Analysis on Human Adipose Tissue-Derived Mesenchymal Stem Cells

Titanium dioxide (TiO2) is manufactured worldwide as crystalline and amorphous forms for multiple applications, including tissue engineering, but our study proposes analyzing the impact of crystalline phases of TiO2 on Mesenchymal Stem Cells (MSCs). Several studies have already described the regenerative potential of MSCs and TiO2 has been used for bone regeneration. In this study, polydispersity index and sizes of TiO2 nanocrystals (NCs) were determined. Adipose tissue-derived Mesenchymal Stem Cells (AT-MSCs) were isolated and characterized in order to evaluate cellular viability and the internalization of nanocrystals (NCs). All of the assays were performed using the TiO2 NCs with 100% anatase (A), 91.6% anatase/9.4% rutile (AR), 64.6% rutile/35.4% anatase (RA), and 84.0% rutile/16% brookite (RB), submitted to several concentrations in 24-h treatments. Cellular localization of TiO2 NCs in the AT-MSCs was resolved by europium-doped NCs. Viability was significantly improved under the predominance of the rutile phase in NCs with localization restricted at the cytoplasm, suggesting that AR and RA NCs are not genotoxic and can be associated with most cellular activities and metabolic pathways, including glycolysis and cell division.

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"In vitro" and multicolor phenotypic characterization of cell subpopulations identified in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells

Journal of Translational Medicine ◽

10.1186/1479-5876-5-55 ◽

2007 ◽

Vol 5 (1) ◽

Cited By ~ 111

Author(s):

Giuseppe Astori ◽

Francesca Vignati ◽

Silvana Bardelli ◽

Monica Tubio ◽

Mauro Gola ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Stromal Vascular Fraction ◽

Human Adipose Tissue ◽

Phenotypic Characterization ◽

Cell Subpopulations

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ACitrus bergamiaExtract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue

PPAR Research ◽

10.1155/2016/4563815 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Debora Lo Furno ◽

Adriana Carol Eleonora Graziano ◽

Rosanna Avola ◽

Rosario Giuffrida ◽

Vincenzo Perciavalle ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Colorimetric Assay ◽

Adipogenic Differentiation ◽

Human Adipose Tissue ◽

Hormone Sensitive Lipase ◽

Fatty Acid Binding ◽

The Impact ◽

Citrus Bergamia

The aim of this research was to assess the impact of a well-characterized extract fromCitrus bergamiajuice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract. To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added withCitrus bergamiafor further 14 days. To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay.Citrus bergamiaextract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes.Citrus bergamiaextract could be a useful natural product for improving adipose mobilization in obesity-related disorders.

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