scholarly journals MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183238 ◽  
Author(s):  
Sirama Niare ◽  
Lionel Almeras ◽  
Fatalmoudou Tandina ◽  
Amina Yssouf ◽  
Affane Bacar ◽  
...  
2018 ◽  
Vol 63 ◽  
pp. 410-419 ◽  
Author(s):  
Sirama Niare ◽  
Fatalmoudou Tandina ◽  
Bernard Davoust ◽  
Ogobara Doumbo ◽  
Didier Raoult ◽  
...  

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 40 ◽  
Author(s):  
Fatalmoudou Tandina ◽  
Maureen Laroche ◽  
Bernard Davoust ◽  
Ogobara K Doumbo ◽  
Philippe Parola

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently emerged in entomology as a technique to identify arthropods and their blood meal source. In this study, female Anopheles gambiae were fed on five host blood sources: ocelot (Leopardus pardalis), binturong (Arctictis binturong), springbok (Antidorcas marsupialis), jaguar (Panthera onca) and Hamadryas baboon (Papio hamadryas), while Anopheles coluzzii were fed on three hosts: dromedary (Camelus dromedarius), Barbary sheep (Ammotragus lervia) and pig (Sus scrofa). We obtained the MS spectra from 240 engorged mosquito abdomens and selected high quality ones from 72 mosquito abdomens to upgrade our home-made database. We excluded from the analysis any spectra of low quality (n = 80), and the remaining 88 specimens were subjected to a blind test analysis against the home-made database. We obtained 100% correct identification of the blood meal source for the specimens collected, 1, 12 and 24 h post-feeding, whereas for the specimens collected 36 h post-feeding, the correct identification rate decreased dramatically. We confirm here that MALDI-TOF MS can be used to identify the blood meal origin of freshly engorged mosquitoes, which opens new perspectives for further studies, including the impact of the mosquito species on blood meal identification.


Parasitology ◽  
2018 ◽  
Vol 145 (9) ◽  
pp. 1170-1182 ◽  
Author(s):  
Fatalmoudou Tandina ◽  
Sirama Niaré ◽  
Maureen Laroche ◽  
Abdoulaye K Koné ◽  
Adama Z Diarra ◽  
...  

AbstractMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.


2019 ◽  
Vol 100 (1) ◽  
pp. 47-53
Author(s):  
Adama Zan Diarra ◽  
Maureen Laroche ◽  
Franck Berger ◽  
Philippe Parola
Keyword(s):  

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Sirama Niare ◽  
Jean-Michel Berenger ◽  
Constentin Dieme ◽  
Ogobara Doumbo ◽  
Didier Raoult ◽  
...  

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  

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