The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus

This study was carried out to assess the prevalence of gastrointestinal nematode in cattle in the Pintu Rime Gayo, a highland sub district of Bener Meriah Regency, Aceh according to age and sex. Stool samples were collected from 150 cattle aged 0-6, 7-12 and >12 months old. Examination was performed by using a centrifuge. Samples tested positive when found gastrointestinal nematode parasite eggs. The data obtained were analyzed with Chi-Square. The results showed that the prevalence of gastrointestinal nematode in cattle aged 0-7 months (32%) was significantly higher (p<0.05) than that in cattle aged 7-12 months (22%) and >12 months (12%). The parasite was markedly more prevalent (p<0.05) in female cattle (23.9%) than that in male cattle (15.2%). In conclusion, susceptibility of cattle in the Pintu Rime Gayo highland to gastrointestinal nematode infection was influenced by both age and sex.

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Faecal egg counts and immune markers in a line of Scottish Cashmere goats selected for resistance to gastrointestinal nematode parasite infection

Veterinary Parasitology ◽

10.1016/j.vetpar.2016.08.027 ◽

2016 ◽

Vol 229 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

David McBean ◽

Mintu Nath ◽

Fiona Kenyon ◽

Karina Zile ◽

David J. Bartley ◽

...

Keyword(s):

Gastrointestinal Nematode ◽

Parasite Infection ◽

Nematode Parasite ◽

Immune Markers ◽

Cashmere Goats ◽

Faecal Egg Counts ◽

Gastrointestinal Nematode Parasite

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IL-13, IL-4Rα, and Stat6 Are Required for the Expulsion of the Gastrointestinal Nematode Parasite Nippostrongylus brasiliensis

Immunity ◽

10.1016/s1074-7613(00)80477-x ◽

1998 ◽

Vol 8 (2) ◽

pp. 255-264 ◽

Cited By ~ 410

Author(s):

Joseph F Urban ◽

Nancy Noben-Trauth ◽

Debra D Donaldson ◽

Kathleen B Madden ◽

Suzanne C Morris ◽

...

Keyword(s):

Gastrointestinal Nematode ◽

Nematode Parasite ◽

Nippostrongylus Brasiliensis ◽

Gastrointestinal Nematode Parasite

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Cloning and characterization of a β-galactoside-binding protein (galectin) from the gut of the gastrointestinal nematode parasite Haemonchus contortus

Parasitology ◽

10.1017/s003118209900503x ◽

1999 ◽

Vol 119 (5) ◽

pp. 483-490 ◽

Cited By ~ 29

Author(s):

G. F. J. NEWLANDS ◽

P. J. SKUCE ◽

D. P. KNOX ◽

S. K. SMITH ◽

W. D. SMITH

Keyword(s):

Haemonchus Contortus ◽

Gastrointestinal Nematode ◽

Protective Capacity ◽

Membrane Protein Complex ◽

Repeat Structure ◽

Sds Page ◽

Protection Against Infection ◽

Binding Lectin ◽

Gastrointestinal Nematode Parasite

A cDNA encoding a β-galactoside-binding lectin (galectin) was identified by immunoscreening a Haemonchus contortus cDNA library with antisera from lambs vaccinated with a membrane protein complex (H-gal-GP) derived from the parasites' gut. The cDNA sequence, exhibiting a tandem repeat structure and designated Hco-gal-2, showed significant levels of similarity with galectins from several species of nematode as well as mammalian galectin type 4. Native galectin was preferentially extracted from the H-gal-GP complex and also from an insoluble membrane fraction prepared from adult worms using lactose–agarose affinity chromatography. The affinity-purified material had apparent molecular mass of around 35 kDa with 3 distinct bands visible on SDS–PAGE. All 3 bands were identified as galectins by reaction with antiserum raised to recombinant Hco-GAL-2 on Western blot. To determine whether H. contortus galectins have any protective capacity against infection lambs were vaccinated with affinity-purified galectin and subsequently given a single challenge infection. Vaccination did not confer any protection against infection with H. contortus as judged by faecal egg output or worm counts.

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