scholarly journals Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0224878 ◽  
Author(s):  
Sarah H. Needs ◽  
Tai The Diep ◽  
Stephanie P. Bull ◽  
Anton Lindley-Decaire ◽  
Partha Ray ◽  
...  
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Time Lapse ◽  
3D Printer ◽  
Throughput Time ◽  
Time Lapse Imaging

scholarly journals Automated profiling of individual cell–cell interactions from high-throughput time-lapse imaging microscopy in nanowell grids (TIMING)

2015 ◽  
Vol 31 (19) ◽  
pp. 3189-3197 ◽  
Author(s):  
Amine Merouane ◽  
Nicolas Rey-Villamizar ◽  
Yanbin Lu ◽  
Ivan Liadi ◽  
Gabrielle Romain ◽  
...  
Keyword(s):  
High Throughput ◽  
Individual Cell ◽  
Time Lapse ◽  
Cell Interactions ◽  
Throughput Time ◽  
Time Lapse Imaging ◽  
Cell Cell

scholarly journals Time-lapse imaging of molecular evolution by high-throughput sequencing

2018 ◽  
Vol 46 (15) ◽  
pp. 7480-7494 ◽  
Author(s):  
Nam Nguyen Quang ◽  
Clément Bouvier ◽  
Adrien Henriques ◽  
Benoit Lelandais ◽  
Frédéric Ducongé
Keyword(s):  
Molecular Evolution ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Time Lapse ◽  
Time Lapse Imaging

scholarly journals High-throughput live-imaging of embryos in microwell arrays using a modular, inexpensive specimen mounting system

2017 ◽  
Author(s):  
Seth Donoughe ◽  
Chiyoung Kim ◽  
Cassandra G. Extavour
Keyword(s):  
High Throughput ◽  
Fruit Fly ◽  
Time Lapse ◽  
Live Imaging ◽  
Model Systems ◽  
Wide Range ◽  
Amphipod Crustacean ◽  
Live Image ◽  
Time Lapse Imaging ◽  
Annelid Worm

AbstractLive-imaging embryos in a high-throughput manner is essential for shedding light on a wide range of questions in developmental biology, but it is difficult and costly to mount and image embryos in consistent conditions. Here, we present OMMAwell, a simple, reusable device that makes it easy to mount up to hundreds of embryos in arrays of agarose microwells with customizable dimensions and spacing. OMMAwell can be configured to mount specimens for upright or inverted microscopes, and includes a reservoir to hold live-imaging medium to maintain constant moisture and osmolarity of specimens during time-lapse imaging. All device components can be cut from a sheet of acrylic using a laser cutter. Even a novice user will be able to cut the pieces and assemble the device in less than an hour. At the time of writing, the total materials cost is less than five US dollars. We include all device design files in a commonly used format, as well as complete instructions for its fabrication and use. We demonstrate a detailed workflow for designing a custom mold and employing it to simultaneously live-image dozens of embryos at a time for more than five days, using embryos of the cricket Gryllus bimaculatus as an example. Further, we include descriptions, schematics, and design files for molds that can be used with 14 additional vertebrate and invertebrate species, including most major traditional laboratory models and a number of emerging model systems. Molds have been user-tested for embryos including zebrafish (Danio rerio), fruit fly (Drosophila melanogaster), coqui frog (Eleutherodactylus coqui), annelid worm (Capitella teleta), amphipod crustacean (Parhyale hawaiensis), red flour beetle (Tribolium castaneum), and three-banded panther worm (Hofstenia miamia), as well mouse organoids (Mus musculus). Finally, we provide instructions for researchers to customize OMMAwell inserts for embryos or tissues not described herein.Summary StatementThis Techniques and Resources article describes an inexpensive, customizable device for mounting and live-imaging a wide range of tissues and species; complete design files and instructions for assembly are included.

Mother machine image analysis with MM3

2019 ◽  
Author(s):  
John T. Sauls ◽  
Jeremy W. Schroeder ◽  
Steven D. Brown ◽  
Guillaume Le Treut ◽  
Fangwei Si ◽  
...  
Keyword(s):  
Image Analysis ◽  
Phase Contrast ◽  
Time Lapse ◽  
Command Line ◽  
Throughput Time ◽  
Analysis Pipeline ◽  
Machine Experiment ◽  
Command Line Tool ◽  
Time Lapse Imaging ◽  
Machine Image

The mother machine is a microfluidic device for high-throughput time-lapse imaging of microbes. Here, we present MM3, a complete and modular image analysis pipeline. MM3 turns raw mother machine images, both phase contrast and fluorescence, into a data structure containing cells with their measured features. MM3 employs machine learning and non-learning algorithms, and is implemented in Python. MM3 is easy to run as a command line tool with the occasional graphical user interface on a PC or Mac. A typical mother machine experiment can be analyzed within one day. It has been extensively tested, is well documented and publicly available via Github.

scholarly journals A generic methodological framework for studying single cell motility in high-throughput time-lapse data

2015 ◽  
Vol 31 (12) ◽  
pp. i320-i328 ◽  
Author(s):  
Alice Schoenauer Sebag ◽  
Sandra Plancade ◽  
Céline Raulet-Tomkiewicz ◽  
Robert Barouki ◽  
Jean-Philippe Vert ◽  
...  
Keyword(s):  
Cell Motility ◽  
Single Cell ◽  
High Throughput ◽  
Time Lapse ◽  
Methodological Framework ◽  
Throughput Time

scholarly journals Low-Cost Automated Vectors and Modular Environmental Sensors for Plant Phenotyping

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3319
Author(s):  
Stuart A. Bagley ◽  
Jonathan A. Atkinson ◽  
Henry Hunt ◽  
Michael H. Wilson ◽  
Tony P. Pridmore ◽  
...  
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Time Lapse ◽  
Plant Phenotyping ◽  
Environmental Sensors ◽  
Controlled Conditions ◽  
Sensor Platform ◽  
High Throughput Phenotyping ◽  
Controlled Environments ◽  
Time Lapse Imaging

High-throughput plant phenotyping in controlled environments (growth chambers and glasshouses) is often delivered via large, expensive installations, leading to limited access and the increased relevance of “affordable phenotyping” solutions. We present two robot vectors for automated plant phenotyping under controlled conditions. Using 3D-printed components and readily-available hardware and electronic components, these designs are inexpensive, flexible and easily modified to multiple tasks. We present a design for a thermal imaging robot for high-precision time-lapse imaging of canopies and a Plate Imager for high-throughput phenotyping of roots and shoots of plants grown on media plates. Phenotyping in controlled conditions requires multi-position spatial and temporal monitoring of environmental conditions. We also present a low-cost sensor platform for environmental monitoring based on inexpensive sensors, microcontrollers and internet-of-things (IoT) protocols.

High-throughput RNAi screening by time-lapse imaging of live human cells

2006 ◽  
Vol 3 (5) ◽  
pp. 385-390 ◽  
Author(s):  
Beate Neumann ◽  
Michael Held ◽  
Urban Liebel ◽  
Holger Erfle ◽  
Phill Rogers ◽  
...  
Keyword(s):  
High Throughput ◽  
Time Lapse ◽  
Human Cells ◽  
Rnai Screening ◽  
Time Lapse Imaging

scholarly journals CellProfiler Tracer: exploring and validating high-throughput, time-lapse microscopy image data

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Mark-Anthony Bray ◽  
Anne E. Carpenter
Keyword(s):  
High Throughput ◽  
Image Data ◽  
Time Lapse ◽  
Throughput Time ◽  
Time Lapse Microscopy ◽  
Microscopy Image
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