scholarly journals Magnetophoretic and spectral characterization of oxyhemoglobin and deoxyhemoglobin: Chemical versus enzymatic processes

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257061
Author(s):  
Mitchell R. H. Weigand ◽  
Jenifer Gómez-Pastora ◽  
James Kim ◽  
Matthew T. Kurek ◽  
Richard J. Hickey ◽  
...  
Keyword(s):  
Time Course ◽  
Cell Tracking ◽  
Sodium Dithionite ◽  
Side Reactions ◽  
Preparation Methods ◽  
Visible Spectra ◽  
Uv Visible ◽  
Cell Tracking Velocimetry ◽  
Human Rbcs

A new method for hemoglobin (Hb) deoxygenation, in suspension or within red blood cells (RBCs) is described using the commercial enzyme product, EC-Oxyrase®. The enzymatic deoxygenation method has several advantages over established deoxygenation methodologies, such as avoiding side reactions that produce methemoglobin (metHb), thus eliminating the need for an inert deoxygenation gas and airtight vessel, and facilitates easy re-oxygenation of Hb/RBCs by washing with a buffer that contains dissolved oxygen (DO). The UV-visible spectra of deoxyHb and metHb purified from human RBCs using three different preparation methods (sodium dithionite [to produce deoxyHb], sodium nitrite [to produce metHb], and EC-Oxyrase® [to produce deoxyHb]) show the high purity of deoxyHb prepared using EC-Oxyrase® (with little to no metHb or hemichrome production from side reactions). The oxyHb deoxygenation time course of EC-Oxyrase® follows first order reaction kinetics. The paramagnetic characteristics of intracellular Hb in RBCs were compared using Cell Tracking Velocimetry (CTV) for healthy and sickle cell disease (SCD) donors and oxygen equilibrium curves show that the function of healthy RBCs is unchanged after EC-Oxyrase® treatment. The results confirm that this enzymatic approach to deoxygenation produces pure deoxyHb, can be re-oxygenated easily, prepared aerobically and has similar paramagnetic mobility to existing methods of producing deoxyHb and metHb.

Spectroelectrochemical characterization of meso triaryl-substituted Mn(IV), Mn(III) and Mn(II) corroles. Effect of solvent and oxidation state on UV-visible spectra and redox potentials in nonaqueous media

2014 ◽  
Vol 18 (12) ◽  
pp. 1131-1144 ◽  
Author(s):  
Bingbing Gao ◽  
Zhongping Ou ◽  
Xueyan Chen ◽  
Shi Huang ◽  
Bihong Li ◽  
...  
Keyword(s):  
Anion Radical ◽  
Cation Radical ◽  
Oxidation Mechanism ◽  
Central Metal ◽  
Redox Potentials ◽  
Nonaqueous Media ◽  
Visible Spectra ◽  
Phenyl Rings ◽  
Uv Visible

Two series of substituted manganese triarylcorroles were synthesized and characterized as to their electrochemical and spectroelectrochemical properties in CH 2 Cl 2, CH 3 CN and pyridine. The investigated compounds are represented as ( YPh )3 CorMn III and ( YPh )3 CorMn IV Cl , where Cor is a trianion of the corrole and Y is a Cl , F , H or CH 3 para-substituent on the three phenyl rings of the macrocycle. Each neutral Mn(III) corrole exists as a four-coordinate complex in CH 2 Cl 2 and CH 3 CN and as a five-coordinate species in pyridine. ( YPh )3 CorMn III undergoes two oxidations to stepwise generate a Mn(IV) corrole and a Mn(IV) π-cation radical. It also undergoes one reduction to generate a Mn(II) corrole in CH 2 Cl 2 or CH 3 CN . In contrast, the reduction of ( YPh )3 CorMn III leads to a Mn(III) corrole π-anion radical in pyridine. One oxidation is observed for ( YPh )3 CorMn IV Cl in CH 2 Cl 2 and CH 3 CN to generate a Mn(IV) corrole π-cation radical while Mn(III) and Mn(II) corroles are stepwise formed after reduction of the same compound. The second reduction of ( YPh )3 CorMn IV Cl in pyridine gives a Mn(III) π-anion radical as opposed to a Mn(II) corrole with an unreduced π-ring system. The neutral, reduced and oxidized forms of each corrole were characterized by electrochemistry and UV-visible spectroelectrochemistry and comparisons are made between the UV-visible spectra and redox potentials of the compounds in different central metal oxidation states. An overall reduction/oxidation mechanism in the three solvents is proposed.

Characterization of antibody binding to three cancer-related antigens using flow cytometry and cell tracking velocimetry

2003 ◽  
Vol 82 (3) ◽  
pp. 340-351 ◽  
Author(s):  
E. Julia Chosy ◽  
Masayuki Nakamura ◽  
Kristie Melnik ◽  
Kristin Comella ◽  
Larry C. Lasky ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Tracking ◽  
Antibody Binding ◽  
Cell Tracking Velocimetry

SYNTHESIS AND CHARACTERIZATION OF THIOPHENE-CONTAINING CHROMOPHORES FOR NONLINEAR OPTICAL (NLO) MATERIALS

2008 ◽  
Vol 17 (03) ◽  
pp. 243-254 ◽  
Author(s):  
ZUOSEN SHI ◽  
XIAOLONG ZHANG ◽  
ZHANCHEN CUI
Keyword(s):  
Absorption Maximum ◽  
Nonlinear Optical ◽  
Synthesis And Characterization ◽  
Knoevenagel Reaction ◽  
Scanning Calorimetry ◽  
Visible Spectra ◽  
Nlo Materials ◽  
Ft Ir ◽  
Uv Visible

A series of chromophores based on benzene and thiophene moieties as π-conjugating spacers were designed and synthesized by Horner-Emmons-Wadsworth reaction and Vilsmeier-Haack reaction, followed by Knoevenagel reaction with different electron acceptors. Their structures were confirmed by 1HNMR, FT-IR, HPLC-Ms, UV-visible spectra, and the thermal properties were characterized by differential scanning calorimetry (DSC). Solvatochromic method was used to measure their nonlinear optical properties. The variation of chromophoric structures on NLO properties was investigated, indicating that the cyano substitution on the vinylene bridge of the chromophore produces a considerable red-shift of the absorption maximum, and would greatly enhance the molecular quadratic hyperpolarizability (βμ).

scholarly journals Synthesis and Characterization of Some New Metals Complexes of [N-(4-Nitrobenzoyl Amino)-Thioxomethyl] Phenylalanine

2016 ◽  
Vol 13 (1) ◽  
pp. 113-121
Author(s):  
Baghdad Science Journal
Keyword(s):  
Molecular Structure ◽  
Magnetic Susceptibility ◽  
Palladium Complexes ◽  
Synthesis And Characterization ◽  
Conductivity Measurements ◽  
Tetrahedral Geometry ◽  
Visible Spectra ◽  
Ft Ir ◽  
Uv Visible

A new ligand [N-(4-nitrobenzoylamino)-thioxomethyl] phenylalanine is synthesized by reaction of 4-nitrobenzoyl isothiocyanate with phenylalanine (1:1). It is characterized by micro elemental analysis (C.H.N.S.), FT-IR, (UV-Vis) and 1H and 13CNMR spectra. Some metals ions complexes of this ligand were prepared and characterized by FT-IR, UV-Visible spectra, conductivity measurements, magnetic susceptibility and atomic absorption. From results obtained, the following formula [M(NBA)2] where M2+ = Mn, Co, Ni, Cu, Zn, Pd, Cd and Hg, the proposed molecular structure for these complexes as tetrahedral geometry, except copper and palladium complexes are have square planer geometry.

Synthesis and Characterization of a New Technetium(V) Complex with Butane-2,3-dionemonoxime and Some PPP Calculations

1991 ◽  
Vol 46 (5) ◽  
pp. 662-664
Author(s):  
Mostafa K. Hassan ◽  
Anwar S. El-Shahawy ◽  
Refaat M. Mahfouz ◽  
Ahmed H. Osman ◽  
Refeat M. Mahfouz
Keyword(s):  
Dipole Moment ◽  
Synthesis And Characterization ◽  
Mo Calculations ◽  
Molecular Dipole ◽  
Charge Densities ◽  
Visible Spectra ◽  
Uv Visible ◽  
Ci Calculation ◽  
Hplc Data

Using a PPP(SCF)—CI calculation, the structure of the butane-2,3-dionemonoxime ligand has been determined. Via SCF—MO calculations, the ionization potential, the electron affinity, the charge densities in the three states (a molecule, a cation and an anion), the SCF-HO-MO’s and the SCF-LUMO’s were calculated in addition to the molecular dipole moment. A new technetium(V) oxocomplex with butane-2,3-dionemonoxime ligand CH3COCNOHCH3 (diacetylmonoxime) has been synthesized and characterized. The complex formation was performed in an alkaline mixture of water and ethanol by reduction of pertechnetate with NaBH4 in the presence of the monoxime ligand. Infrared, UV-visible spectra, electrophoresis and HPLC data of the resulting complex are reported and discussed.

scholarly journals Synthesis and Characterization of CeVO4by Microwave Radiation Method and Its Photocatalytic Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Nuengruethai Ekthammathat ◽  
Titipun Thongtem ◽  
Anukorn Phuruangrat ◽  
Somchai Thongtem
Keyword(s):  
Synthesis Method ◽  
Synthesis And Characterization ◽  
Strong Emission ◽  
Phase Purity ◽  
Energy Gaps ◽  
Visible Spectra ◽  
Direct Energy ◽  
Uv Visible ◽  
Microwave Radiation Method

A general microwave synthesis method was developed to synthesize cerium orthovanadate (CeVO4) nanostructures without the use of any catalysts or templates. This method is able to control the shape and size of the products by adjusting the pH of precursor solutions to be 1–10. Phase, purity, and different morphologies of the products were characterized by XRD, FTIR, SEM, and TEM. They showed that the as-synthesized products exhibited pure single crystalline CeVO4with tetragonal structure. Their morphologies developed in sequence as nanoparticles (pH = 4–10), nanorods (pH = 2, 3), and microflowers (pH = 1). UV-visible spectra were used to estimate the direct energy gaps of CeVO4nanorods and microflowers: 3.77 and 3.65 eV, respectively. Photoluminescence (PL) of CeVO4microflowers showed strong emission intensities at 578 nm. These results were in the range of possible application for photocatalysis, investigated by studying the degradation of methylene blue.

scholarly journals Synthesis and characterization of Graphene produced from Iraqi date syrup

2019 ◽  
Vol 26 (1) ◽  
pp. 49-54
Author(s):  
Hasan Hasan Makki ◽  
Hameed Hussein Alwan
Keyword(s):  
Thermal Reduction ◽  
Synthesis And Characterization ◽  
Preparation Methods ◽  
Xrd Pattern ◽  
Date Syrup ◽  
Ft Ir ◽  
Concentrated Sulfuric Acid ◽  
Uv Visible ◽  
Dehydration Agent

Graphene is a mono layer of hexagonal honey structure of carbon atoms. Iraqi date syrup was used as a carbon source for synthesis Graphene by expanded dehydration method, in which using of concentrated sulfuric acid as dehydration agent. The method name refers to expanded dehydration because of the increasing in volume with dehydration reaction. Characterization the prepared Graphene was done by FT-IR, XRD, SEM, AFM and UV-Visible techniques and compared with previous work. The final product was obtained by annealing at 600 °C or in other word thermal reduction to reduce all oxide groups combined with structure.  Graphene XRD pattern shows wide peak at 002 plane centered at 2θ =23.758°, and d-spacing = 3.37 Å. AFM image shows that prepared Graphene in Nano sheets form. The method exhibit production yield about 9% and this is relatively high in comparison with other Graphene preparation methods.

Fabrication and Characterization of Amine Compounds Synthesized from Carbon Dioxide and Ammonia Water Using Transition Metal Doped TiO2

2015 ◽  
Vol 764-765 ◽  
pp. 47-50
Author(s):  
Amir Abidov ◽  
Bunyod Allabergenov ◽  
Fei Yi Xiao ◽  
Xing Jin ◽  
Soon Wook Jeong ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Transition Metal ◽  
High Performance ◽  
Main Product ◽  
Visible Spectra ◽  
Ammonia Aqueous Solution ◽  
Uv Visible ◽  
Metal Doped ◽  
Amine Compounds

Capture and reforming fume gases from atmosphere became an ultimate task recently. Photocatalytic reduction to useful compounds is a key to the future. Photoinduced reduction of carbon dioxide and ammonia aqueous solution into amine compounds at room temperature and atmospheric pressure using transition metal doped titanium dioxide is reported in this paper. UV-visible spectra showed a high absorbance at 200 nm wavelength which is a characteristic of urea. High Performance Liquid Chromatography revealed presence of amine compounds and urea was found a main product of photocatalytic reaction.

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