Efficacy of Interferon Gamma Release Assay for Differential Diagnosis of Pulmonary Tuberculosis and Nontuberculous Mycobacterial Lung Disease

Background: Assessment of the effectiveness of tuberculosis control strategies requires the periodic measurement of M. tuberculosis transmission in populations, which is notoriously difficult. One well-established method is to measure the prevalence of infectious pulmonary tuberculosis in the population which is then repeated at a second time point after a period of ‘intervention’, such as scale up of the Search-Treat-Prevent strategy of the Zero TB Cities initiative, allowing for a ‘before and after’ comparison. Protocol: The concurrent adult pulmonary tuberculosis prevalence survey (using digital radiography and Xpert MTB/RIF Ultra) and child M. tuberculosis infection survey (using QuantiFERON-TB® Gold Plus) will primarily provide a baseline measure of the burden of adult infectious tuberculosis in Karachi and assess whether a large-scale interferon gamma release assay survey in children aged 2 to 4 years is feasible. The target population for the prevalence survey is comprised of a stratified random sample of all adults aged 15 years and above and all children aged 2 to 4 years resident in four districts in Karachi. The survey procedures and analyses to estimate pulmonary tuberculosis prevalence are based on the World Health Organization methodology for tuberculosis prevalence surveys. Ethics and dissemination: The study protocol has been approved by the Interactive Research Development / The Indus Hospital Research Centre Research Ethics Committee in Karachi, Pakistan and the London School of Hygiene & Tropical Medicine Research Ethics Committee. Due to non-representative sampling in this setting, where a large proportion of the population are illiterate and are reluctant to provide fingerprints due to concerns about personal security, verbal informed consent will be obtained from each eligible participant or guardian. Results will be submitted to international peer-reviewed journals, presented at international conferences and shared with participating communities and with the Provincial and National TB programme.

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Negative effect of ferritin levels on T-SPOT.TB, an enzyme-linked immunospot based interferon-gamma release assay using Mycobacterium tuberculosis specific antigens, in the smear-positive active pulmonary tuberculosis.

10.1183/13993003.congress-2018.pa4775 ◽

2018 ◽

Author(s):

Masaki Yamamoto ◽

Ken Tashiro ◽

Misako Ikeda ◽

Hideyuki Nagakura ◽

Kentaro Nakashima ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Interferon Gamma ◽

Interferon Gamma Release Assay ◽

Active Pulmonary Tuberculosis ◽

Negative Effect ◽

Release Assay ◽

Specific Antigens

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Cost-effectiveness of M. tuberculosis specific Interferon-gamma release assay testing for the treatment of latent tuberculosis infection in Switzerland

Pneumologie ◽

10.1055/s-2007-973117 ◽

2007 ◽

Vol 61 (S 1) ◽

Author(s):

R Diel ◽

P Wrighton-Smith ◽

J Zellweger

Keyword(s):

Cost Effectiveness ◽

Interferon Gamma ◽

Latent Tuberculosis Infection ◽

Latent Tuberculosis ◽

Tuberculosis Infection ◽

Interferon Gamma Release Assay ◽

Release Assay

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The value of single or combined use of pleural fluid interferon gamma release assay in the diagnosis of tuberculous pleurisy

Tropical Medicine & International Health ◽

10.1111/tmi.13659 ◽

2021 ◽

Author(s):

Xiang Tong ◽

Zhenzhen Li ◽

Junjie Zhao ◽

Sitong Liu ◽

Hong Fan

Keyword(s):

Pleural Fluid ◽

Interferon Gamma ◽

Interferon Gamma Release Assay ◽

Combined Use ◽

Release Assay ◽

Tuberculous Pleurisy

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Role of interferon gamma release assay in the diagnosis and management of Mycobacterium tuberculosis-associated uveitis: a review

BMJ Open Ophthalmology ◽

10.1136/bmjophth-2020-000663 ◽

2021 ◽

Vol 6 (1) ◽

pp. e000663

Author(s):

Samra Rahman ◽

Muhammad Irfan ◽

M A Rehman Siddiqui

Keyword(s):

Interferon Gamma ◽

Interferon Gamma Release Assay ◽

Limited Evidence ◽

Ocular Tuberculosis ◽

Diagnosis And Management ◽

Pubmed Database ◽

Interferon Gamma Release Assays ◽

Infectious Uveitis ◽

Release Assay

Tuberculosis (TB)-associated uveitis is a common cause of infectious uveitis in the developing world. Diagnosis of TB uveitis remains a challenge. The role of interferon gamma release assays (IGRAs) is uncertain. Herein we summarise the available literature on the utility of IGRAs in the diagnosis and management of TB uveitis. We searched PubMed database from 1 August 2010 to 31 July 2020 using the following keywords alone and in combination: ‘interferon-gamma release assay’, ‘QuantiFERON’, ‘T-SPOT.TB’, ‘TB uveitis’, ‘serpiginous like choroiditis’, ‘tuberculoma’, ‘TB vasculitis’, ‘TB panuveitis’ and ‘ocular tuberculosis’. Data from 58 relevant studies were collated. The review is focused on currently marketed versions of IGRA tests: QuantiFERON-TB Gold In-Tube assay, QuantiFERON-TB Gold Plus assay (QFT-Plus) and T-SPOT.TB. We found limited evidence regarding the diagnostic utility of IGRA in patients with uveitis. No study was identified evaluating the newer QFT test—the QFT-Plus—in patients with uveitis. Similarly, there is lack of data directly comparing QFT-Plus with T-SPOT.TB specifically for the diagnosis of TB uveitis.

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