Linear Amplification Sequencing with Dye Terminators

2003 ◽  
pp. 281-296 ◽  
Author(s):  
André Rosenthal ◽  
D. Stephen Charnock-Jones
Keyword(s):  
Linear Amplification ◽  
Dye Terminators

Improvement of Carrier Power to Third-Order Intermodulation Distortion Power Ratio for a Power Amplifier at 5.25 GHz using LINC Method

2018 ◽  
Vol 12 (1) ◽  
pp. 34-40
Author(s):  
Said Elkhaldi ◽  
Naima Amar Touhami ◽  
Mohamed Aghoutane ◽  
Taj-eddin Elhamadi
Keyword(s):  
Power Amplifier ◽  
Artificial Neural Network Model ◽  
Power Ratio ◽  
Linear Amplification ◽  
Third Order ◽  
Gaas Mesfet ◽  
Constant Envelope ◽  
Modulation Signal ◽  
Low Efficiency ◽  
Nonlinear Components

Introduction:This paper focuses on improving the power amplifier linearity for wireless communications. The use of a single branch of a power amplifier can produce high distortion with low efficiency.Method:In this paper, the Linear Amplification with Nonlinear Components (LINC) technique is used to improve the linearity and efficiency of the power amplifier. The LINC technique is based on converting the envelope modulation signal into two constant envelope phase-modulated baseband signals. After amplification and combining the resulting signals, the required linear output signal is obtained. To validate the proposed approach, LINC technique is used for linearizing an amplifier based on a GaAs MESFET (described by an artificial neural network Model).Conclusion:Good results have been achieved, and an improvement of about 40.80 dBc and 47.50 dBc respectively is obtained for the Δlower C/I and Δupper C/I at 5.25 GHz.

scholarly journals Perception of Auditory Distance in Normal-Hearing and Moderate-to-Profound Hearing-Impaired Listeners

2019 ◽  
Vol 23 ◽  
pp. 233121651988761 ◽  
Author(s):  
Gilles Courtois ◽  
Vincent Grimaldi ◽  
Hervé Lissek ◽  
Philippe Estoppey ◽  
Eleftheria Georganti
Keyword(s):  
Hearing Loss ◽  
Hearing Aids ◽  
Normal Hearing ◽  
Hearing Impaired ◽  
Spatial Cues ◽  
Linear Amplification ◽  
Profound Hearing Loss ◽  
Wireless Microphone ◽  
Auditory Distance ◽  
Non Linear

The auditory system allows the estimation of the distance to sound-emitting objects using multiple spatial cues. In virtual acoustics over headphones, a prerequisite to render auditory distance impression is sound externalization, which denotes the perception of synthesized stimuli outside of the head. Prior studies have found that listeners with mild-to-moderate hearing loss are able to perceive auditory distance and are sensitive to externalization. However, this ability may be degraded by certain factors, such as non-linear amplification in hearing aids or the use of a remote wireless microphone. In this study, 10 normal-hearing and 20 moderate-to-profound hearing-impaired listeners were instructed to estimate the distance of stimuli processed with different methods yielding various perceived auditory distances in the vicinity of the listeners. Two different configurations of non-linear amplification were implemented, and a novel feature aiming to restore a sense of distance in wireless microphone systems was tested. The results showed that the hearing-impaired listeners, even those with a profound hearing loss, were able to discriminate nearby and far sounds that were equalized in level. Their perception of auditory distance was however more contracted than in normal-hearing listeners. Non-linear amplification was found to distort the original spatial cues, but no adverse effect on the ratings of auditory distance was evident. Finally, it was shown that the novel feature was successful in allowing the hearing-impaired participants to perceive externalized sounds with wireless microphone systems.

Erratum: Linear amplification using envelope feedback

1971 ◽  
Vol 7 (10) ◽  
pp. 284-284
Author(s):  
T. Arthanayake ◽  
H.B. Wood
Keyword(s):  
Linear Amplification

Testing non-linear amplification factors used in ground motion models

2021 ◽  
Author(s):  
Karina Loviknes ◽  
Danijel Schorlemmer ◽  
Fabrice Cotton ◽  
Sreeram Reddy Kotha
Keyword(s):  
Ground Motion ◽  
Site Effects ◽  
Ground Motions ◽  
Site Amplification ◽  
Building Codes ◽  
Linear Amplification ◽  
Motion Models ◽  
Earthquake Predictability ◽  
Non Linear ◽  
Ground Motion Models

<p>Non-linear site effects are mainly expected for strong ground motions and sites with soft soils and more recent ground-motion models (GMM) have started to include such effects. Observations in this range are, however, sparse, and most non-linear site amplification models are therefore partly or fully based on numerical simulations. We develop a framework for testing of non-linear site amplification models using data from the comprehensive Kiban-Kyoshin network in Japan. The test is reproducible, following the vision of the Collaboratory for the Study of Earthquake Predictability (CSEP), and takes advantage of new large datasets to evaluate <span>whether or not</span> non-linear site effects predicted by site-amplification models are supported by empirical data. The site amplification models are tested using residuals between the observations and predictions from a GMM based only on magnitude and distance. When the GMM is derived without any site term, the site-specific variability extracted from the residuals is expected to capture the site response of a site. The non-linear site amplification models are tested against a linear amplification model on individual well-record<span>ing</span> stations. Finally, the result is compared to building codes where non-linearity is included. The test shows that for most of the sites selected as having sufficient records, the non-linear site-amplification models do not score better than the linear amplification model. This suggests that including non-linear site amplification in GMMs and building codes may not yet be justified, at least not in the range of ground motions considered in the test (peak ground acceleration < 0.2 g).</p>

scholarly journals Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ruslan Kalendar ◽  
Alexandr V. Shustov ◽  
Mervi M. Seppänen ◽  
Alan H. Schulman ◽  
Frederick L. Stoddard
Keyword(s):  
Thermal Cycling ◽  
Annealing Temperature ◽  
Phleum Pratense ◽  
Genome Walking ◽  
Palindromic Sequence ◽  
Linear Amplification ◽  
Gene Characterization ◽  
Intron 1 ◽  
Thermal Cycling Profile ◽  
Palindromic Sequences

AbstractGenome walking (GW) refers to the capture and sequencing of unknown regions in a long DNA molecule that are adjacent to a region with a known sequence. A novel PCR-based method, palindromic sequence-targeted PCR (PST-PCR), was developed. PST-PCR is based on a distinctive design of walking primers and special thermal cycling conditions. The walking primers (PST primers) match palindromic sequences (PST sites) that are randomly distributed in natural DNA. The PST primers have palindromic sequences at their 3′-ends. Upstream of the palindromes there is a degenerate sequence (8–12 nucleotides long); defined adapters are present at the 5′-termini. The thermal cycling profile has a linear amplification phase and an exponential amplification phase differing in annealing temperature. Changing the annealing temperature to switch the amplification phases at a defined cycle controls the balance between sensitivity and specificity. In contrast to traditional genome walking methods, PST-PCR is rapid (two to three hours to produce GW fragments) as it uses only one or two PCR rounds. Using PST-PCR, previously unknown regions (the promoter and intron 1) of the VRN1 gene of Timothy-grass (Phleum pratense L.) were captured for sequencing. In our experience, PST-PCR had higher throughput and greater convenience in comparison to other GW methods.

Harnessing asymmetrical substrate recognition by thermostable EndoV to achieve balanced linear amplification in multiplexed SNP typing

2006 ◽  
Vol 84 (2) ◽  
pp. 232-242 ◽  
Author(s):  
Daniel J Turner ◽  
Maneesh R Pingle ◽  
Francis Barany
Keyword(s):  
Dna Sequences ◽  
Bacillus Stearothermophilus ◽  
Thermotoga Maritima ◽  
Dna Amplification ◽  
Linear Amplification ◽  
Strand Displacement Amplification ◽  
Dna Mismatch ◽  
Endonuclease V ◽  
Target Molecules ◽  
Nonspecific Amplification

Multiplexed amplification of specific DNA sequences, by PCR or by strand-displacement amplification, is an intrinsically biased process. The relative abundance of amplified DNA can be altered significantly from the original representation and, in extreme cases, allele dropout can occur. In this paper, we present a method of linear amplification of DNA that relies on the cooperative, sequence-dependent functioning of the DNA mismatch-repair enzyme endonuclease V (EndoV) from Thermotoga maritima (Tma) and Bacillus stearothermophilus (Bst) DNA polymerase. Tma EndoV can nick one strand of unmodified duplex DNA, allowing extension by Bst polymerase. By controlling the bases surrounding a mismatch and the mismatch itself, the efficiency of nicking by EndoV and extension by Bst polymerase can be controlled. The method currently allows 100-fold multiplexed amplification of target molecules to be performed isothermally, with an average change of <1.3-fold in their original representation. Because only a single primer is necessary, primer artefacts and nonspecific amplification products are minimized.Key words: EndoV, endonuclease V, nicking, extension, mismatch, DNA amplification, Bst polymerase.

scholarly journals Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)

Science ◽  
2017 ◽  
Vol 356 (6334) ◽  
pp. 189-194 ◽  
Author(s):  
Chongyi Chen ◽  
Dong Xing ◽  
Longzhi Tan ◽  
Heng Li ◽  
Guangyu Zhou ◽  
...  
Keyword(s):  
Single Cell ◽  
Whole Genome ◽  
Linear Amplification ◽  
Transposon Insertion ◽  
Genome Analyses

Teleportation improvement by noiseless linear amplification

2019 ◽  
Vol 19 (11&12) ◽  
pp. 935-951
Author(s):  
Hamza Adnane ◽  
Matteo G.A. Paris
Keyword(s):  
Coherent States ◽  
Large Range ◽  
Low Energy ◽  
Entangled State ◽  
Continuous Variables ◽  
Linear Amplification ◽  
Gaussian States ◽  
Linear Amplifier ◽  
Twin Beam ◽  
Non Gaussian

We address de-Gaussification of continuous variables Gaussian states by optimal non-deterministic noiseless linear amplifier (NLA) and analyze in details the properties of the amplified states. In particular, we investigate the entanglement content and the non-Gaussian character for the class of non-Gaussian entangled state obtained by using NL-amplification of two-mode squeezed vacua (twin-beam, TWB). We show that entanglement always increases, whereas improved EPR correlations are observed only when the input TWB has low energy. We then examine a Braunstein-Kimble-like protocol for the teleportation of coherent states, and compare the performances of TWB-based teleprotation with those obtained using NL-amplified resources. We show that teleportation fidelity and security may be improved for a large range of NLA parameters (gain and threshold).

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