Extraction and Purification of Plasmid DNA

2003 ◽  
pp. 327-331
Author(s):  
Craig Winstanley ◽  
Ralph Rapley
Keyword(s):  
Plasmid Dna ◽  
Extraction And Purification

AN IMPROVED METHOD FOR THE ASSAY OF PROGESTERONE BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 63 (2) ◽  
pp. 225-241 ◽  
Author(s):  
B. D. Reeves ◽  
M. L. A. de Souza ◽  
I. E. Thompson ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Entire Range ◽  
Improved Method ◽  
Plasma Progesterone ◽  
Extraction And Purification ◽  
Corticosteroid Binding Globulin ◽  
Competitive Protein Binding ◽  
The Individual ◽  
Range Of Values

ABSTRACT An improved method for the assay of plasma progesterone by competitive protein binding is described. The improvement is based upon rigorous control of the variables, the compensation for and standardisation of interfering factors inherent in the method and the use of a human corticosteroid binding globulin, that meets the requirements for sensitivity at levels of 1.0 ng of progesterone and below. The assessment of the reliability of the individual steps in the method as well as that of the complete method is presented. The sensitivity of the method is around 0.2 ng progesterone per ml plasma. Accuracy was measured by adding progesterone in amounts ranging from 0.0 to 1.0 ng to 1.0 ml plasma. There was a linear relationship between the progesterone added and recovered throughout the entire range of values, with a coefficient of correlation (r) of 0.94. Of 52 related steroids tested, none was found which would remain associated with progesterone following extraction and purification and which would also compete with progesterone for binding sites.

Polymeric Delivery of Proteins and Plasmid DNA for Tissue Engineering and Gene Therapy

2001 ◽  
Vol 11 (1-3) ◽  
pp. 12 ◽  
Author(s):  
Thomas P. Richardson ◽  
William L. Murphy ◽  
David J. Mooney
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Plasmid Dna ◽  
Polymeric Delivery

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

Detection of Rhizoctonia solani AG-2-2 IV, the Causal Agent of Large Patch of Zoysiagrass, Using Plasmid DNA as a Probe.

1998 ◽  
Vol 64 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Susumu TAKAMATSU ◽  
Manami NAKANO ◽  
Hideyuki YOKOTA ◽  
Hitoshi KUNOH
Keyword(s):  
Rhizoctonia Solani ◽  
Plasmid Dna ◽  
Causal Agent ◽  
Large Patch
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