Genome-Wide Sreening by Using Small-Interfering RNA Expression Libraries

2006 ◽  
pp. 131-142
Author(s):  
Sahohime Matsumoto ◽  
Makoto Miyagishi ◽  
Kazunari Taira
Keyword(s):  
Small Interfering Rna ◽  
Rna Expression ◽  
Genome Wide ◽  
Interfering Rna ◽  
Expression Libraries

scholarly journals Genome-wide screens identify specific drivers of mutant hTERT promoters

2022 ◽  
Vol 119 (3) ◽  
pp. e2105171119
Author(s):  
Raghuvaran Shanmugam ◽  
Mert Burak Ozturk ◽  
Joo-Leng Low ◽  
Semih Can Akincilar ◽  
Joelle Yi Heng Chua ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Regulatory Region ◽  
Telomerase Activity ◽  
Chromatin Interaction ◽  
Mediator Complex ◽  
Wild Type ◽  
Distal Regulatory Region ◽  
Genome Wide ◽  
Promoter Mutations ◽  
Interfering Rna

Cancer-specific hTERT promoter mutations reported in 19% of cancers result in enhanced telomerase activity. Understanding the distinctions between transcriptional regulation of wild-type (WT) and mutant (Mut) hTERT promoters may open up avenues for development of inhibitors which specially block hTERT expression in cancer cells. To comprehensively identify physiological regulators of WT- or Mut-hTERT promoters, we generated several isogenic reporter cells driven by endogenous hTERT loci. Genome-wide CRISPR-Cas9 and small interfering RNA screens using these isogenic reporter lines identified specific regulators of Mut-hTERT promoters. We validate and characterize one of these hits, namely, MED12, a kinase subunit of mediator complex. We demonstrate that MED12 specifically drives expression of hTERT from the Mut-hTERT promoter by mediating long-range chromatin interaction between the proximal Mut-hTERT promoter and T-INT1 distal regulatory region 260 kb upstream. Several hits identified in our screens could serve as potential therapeutic targets, inhibition of which may specifically block Mut-hTERT promoter driven telomerase reactivation in cancers.

scholarly journals Genome-Wide Small Interfering RNA Screens Reveal VAMP3 as a Novel Host Factor Required for Uukuniemi Virus Late Penetration

2014 ◽  
Vol 88 (15) ◽  
pp. 8565-8578 ◽  
Author(s):  
R. Meier ◽  
A. Franceschini ◽  
P. Horvath ◽  
M. Tetard ◽  
R. Mancini ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Host Factor ◽  
Genome Wide ◽  
Novel Host ◽  
Interfering Rna

scholarly journals Single small-interfering RNA expression vector for silencing multiple transforming growth factor-  pathway components

2005 ◽  
Vol 33 (15) ◽  
pp. e131-e131 ◽  
Author(s):  
A. Jazag ◽  
F. Kanai ◽  
H. Ijichi ◽  
K. Tateishi ◽  
T. Ikenoue ◽  
...  
Keyword(s):  
Growth Factor ◽  
Small Interfering Rna ◽  
Expression Vector ◽  
Transforming Growth Factor ◽  
Rna Expression ◽  
Interfering Rna
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