scholarly journals Clonal propagation of black currant (Ribes nigrum L.)

2020 ◽  
Vol 19 (2) ◽  
pp. 005-010
Author(s):  
K. Yu. Guseva
Keyword(s):  
Gibberellic Acid ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Rooting Medium

At the stage of transfer to soil, a scheme is defined that provides production of improved plantlets of thestudied varieties. 'Kuharsky's Memory' and 'Kanakhama' black сurrant varieties are characterized by high regenerativecapacity in vitro (the proportion of viable explants is 93.5 % and 96.4 %, respectively). At the multiplication stage, the influence of growth regulators (6-benzylaminopurine, gibberellic acid) on the morphogenesis of currant plantlets was studied. It shows their impact on such morphological indicators of development of plantlets as the height (cm), quantity, theaverage length of roots (cm). At the rooting stage, the concentration (0.5 mg/l) of auxin (β-indolylbutyric acid) was selected, the introduction of which into the rooting medium provided the maximum indicators of rhizogenesis: the numberand length of roots.

scholarly journals THE EFFICIENCY OF SHOOT AND PLANTLET FORMATION OF Cephaelis ipecacuanha AFTER THREE SUBCULTURES IN VITRO

1994 ◽  
Vol 24 (3) ◽  
pp. 523-526 ◽  
Author(s):  
Osmar Alves Lameira ◽  
Marly Pedroso da Costa ◽  
José Eduardo Brasil Pereira Pinto
Keyword(s):  
Gibberellic Acid ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Activated Charcoal ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Plantlet Formation ◽  
Cephaelis Ipecacuanha

Multiple adventitious shoot formed from internodal segments of Cephaelis ipecacuanha cultured 25 days on Gamborg basal medium (GAMBORG et al., 1968) supplemented with 6.66mM 6-benzylaminopurine there was a maximum of nine shoots per segment and an average of five shoots per segment formed. The presence of gibberellic acid in the subculture media promoted shoot elongation in all treatments. The shoots attained 3cm in height and rooting of 100% after 35 days of culturing upon Murashige and Skoog's basal medium (MS), added with 4.92mM indole-3-butyric acid, 0.87m gibberellic acid and 0.1% activated charcoal. Further growth was accelerated after the transfer to 1/2 MS without growth regulators. Rooted plantlets transferred to potting soil could be successfully established.

scholarly journals Optimization of the processes of sterilization and micropropagation of cup plant (Silphium perfoliatum L.) from apical explants of seedlings in in vitro cultures

2012 ◽  
Vol 64 (4) ◽  
pp. 3-10 ◽  
Author(s):  
Magdalena Tomaszewska-Sowa ◽  
Anna Figas
Keyword(s):  
Growth Regulators ◽  
Energy Production ◽  
Production Plant ◽  
Perennial Plant ◽  
Rooting Medium ◽  
Lateral Shoots ◽  
Functional Features ◽  
Animal Feeding ◽  
Asteraceae Family

Cup plant (<em>Silphium perfoliatum</em> L.) is a tall, reaching up to 2.5 m, perennial plant that represents the Asteraceae family. <em>Silphium perfoliatum</em> L. can be applied in medicine, animal feeding, and as a decorative, honey-producing and energy production plant which proves its multi-functional features. Sowing material currently available in Poland is insufficient, which justifies the present attempts at propagating these plants in <em>in vitro</em> cultures. Therefore, <em>Silphium perfoliatum</em> L. seed sterilization and micropropagation processes were studied under controlled conditions <em>in in vitro</em> cultures. Among the tested methods, ACE proved to be the most effective for sterilization. The apical parts of seedlings were used as explants; they were placed onto MS media with growth regulators added. 4 growth regulator concentration variants were applied. The highest percentage of explants with lateral shoots (41.7%) and callus (90.3%) was obtained on MS medium with 5 mg x dm<sup>-3</sup> BAP + 1 mg × dm<sup>-3</sup> NAA added. Shoots were isolated and transferred onto MS rooting medium without growth regulators. The rooted plants were transferred to the greenhouse and acclimatised to <em>ex vitro</em> conditions.

In vitro rooting cultivars of black currant (Ribes nigrum L.) of the Bashkir breeding

Biomics ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 332-335 ◽  
Author(s):  
L.A. Golovina ◽  
M.M. Ishmuratova
Keyword(s):  
In Vitro Rooting ◽  
Ribes Nigrum ◽  
Black Currant

scholarly journals In vitro Mass Clonal Propagation of Spathoglottis plicata Blume

1970 ◽  
Vol 19 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Pinaki Sinha ◽  
M. Lokman Hakim ◽  
M. Firoz Alam
Keyword(s):  
Clonal Propagation ◽  
Nodal Segments ◽  
Fluorescent Light ◽  
Plantlet Formation ◽  
The Third ◽  
Regenerated Plants ◽  
Rooting Medium ◽  
Half Strength ◽  
In Vitro Clonal Propagation

For in vitro clonal propagation of Spathoglottis plicata Blume nodal segments of young shoots were cultured on half strength of MS  with  2% sucrose + 2.0 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC,  incubated at 24 ± 2ºC under 3000 lux fluorescent light for a 16 hr photoperiod per day. About 19 micro-shoots were induced from the explants within 12 weeks. Subculture of micro-shoots for eight weeks on the same nutrient medium enhanced the number of micro-shoots up to 60. The clumps of the micro-shoots were dissected and cultured on half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 200 mg/l L-glutamine. The micro-shoot sections elongated to form shoots, and new micro shoots were induced from the base within eight weeks of culture. For plantlet formation the best rooting medium was determined as  half strength of MS  with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 50 g/l banana pulp. After rearing 25 g mixture of urea, TSP and MOP (2 : 1 : 1) were applied per plant at three months intervals. All the regenerated plants blossomed on the third year. Key words: Spathoglottis plicata, Clonal propagation, Acclimation D.O.I. 10.3329/ptcb.v19i2.5432 Plant Tissue Cult. & Biotech. 19(2): 151-160, 2009 (December)

scholarly journals Influence of climatic conditions of the introduction period and varietal characteristics of black currant (Ribes nigrum L.) on the effectiveness of culture initiation in vitro

2021 ◽  
Vol 36 ◽  
pp. 04010
Author(s):  
T.M. Khromova ◽  
L.V. Tashmatova ◽  
O.V. Matsneva ◽  
V.V. Shakhov
Keyword(s):  
Survival Rate ◽  
Physiological State ◽  
Climatic Conditions ◽  
Source Material ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Active Growth ◽  
Murashige Skoog ◽  
One Year

The article presents data from the effectiveness studies of the initial introduction stage of black currant (Ribes nigrum L.) into in vitro culture depending on the introduction period and the corresponding climatic conditions. The research objects were varieties of black currants selected by the Russian Research Institute of Fruit Crop Breeding: Azhurnaya, Orlovskaya serenada, Ocharovanie, Chudnoye mgnovenye. The introduction into in vitro culture was carried out in several periods characterized by different physiological states of the explants: the period of dormancy release (mid-March), the period of active growth (June), and the period of growth decay (mid-September). The source material in the spring and autumn periods were the buds of one-year stiffened shoots, in the summer introduction period - the buds of growing green shoots. The cultivation was carried out on Murashige-Skoog medium supplemented with 6-BAP (0.5 mg/l). It was noted that the survival rate of explants is determined by the physiological state of the source material due to the corresponding agro-climatic conditions during the introduction period, as well as the genotypic characteristics of the varieties. Thus, explants isolated during the active growing season are characterized by a higher and more stable survival rate. When explants were cultivated in spring and autumn, the physiological state of the explants and their survival rate were influenced by the genotypic response of varieties to the corresponding agroclimatic conditions.

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

scholarly journals In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Julián Sebastián Ramírez Moreno ◽  
Sergio Andrés Vega Porras ◽  
Roosevelt Humberto Escobar ◽  
Elena E. Stashenko ◽  
Jorge Luis Fuentes Lorenzo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Nodal Segments ◽  
Ms Medium ◽  
Shoot Number ◽  
Murashige And Skoog Medium

ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.

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