scholarly journals Influence of climatic conditions of the introduction period and varietal characteristics of black currant (Ribes nigrum L.) on the effectiveness of culture initiation in vitro

2021 ◽  
Vol 36 ◽  
pp. 04010
Author(s):  
T.M. Khromova ◽  
L.V. Tashmatova ◽  
O.V. Matsneva ◽  
V.V. Shakhov
Keyword(s):  
Survival Rate ◽  
Physiological State ◽  
Climatic Conditions ◽  
Source Material ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Active Growth ◽  
Murashige Skoog ◽  
One Year

The article presents data from the effectiveness studies of the initial introduction stage of black currant (Ribes nigrum L.) into in vitro culture depending on the introduction period and the corresponding climatic conditions. The research objects were varieties of black currants selected by the Russian Research Institute of Fruit Crop Breeding: Azhurnaya, Orlovskaya serenada, Ocharovanie, Chudnoye mgnovenye. The introduction into in vitro culture was carried out in several periods characterized by different physiological states of the explants: the period of dormancy release (mid-March), the period of active growth (June), and the period of growth decay (mid-September). The source material in the spring and autumn periods were the buds of one-year stiffened shoots, in the summer introduction period - the buds of growing green shoots. The cultivation was carried out on Murashige-Skoog medium supplemented with 6-BAP (0.5 mg/l). It was noted that the survival rate of explants is determined by the physiological state of the source material due to the corresponding agro-climatic conditions during the introduction period, as well as the genotypic characteristics of the varieties. Thus, explants isolated during the active growing season are characterized by a higher and more stable survival rate. When explants were cultivated in spring and autumn, the physiological state of the explants and their survival rate were influenced by the genotypic response of varieties to the corresponding agroclimatic conditions.

DEVELOPMENT OF AN INTRODUCTION PROTOCOL FOR STRAWBERRY PLANTS INTO IN VITRO CULTURE

2020 ◽  
Vol 5 (86) ◽  
pp. 45-50
Author(s):  
O.V. Mazneva ◽  
◽  
L.V. Tashmatova ◽  
T.M. Khromova ◽  
V.V. Shakhov ◽  
...  
Keyword(s):  
Survival Rate ◽  
In Vitro Culture ◽  
Plant Material ◽  
Survival Rates ◽  
Strawberry Plant ◽  
Active Growth ◽  
High Viability ◽  
Average Survival ◽  
Average Survival Rate

The research was conducted in order to develop an effective protocol for introducing strawberry plants into in vitro culture. The objects of the research were the most popular varieties of strawberries of domestic and foreign selection: Tsaritsa, Bereginya, Florence, Frida, Kimberly, etc. Mercurial preparations mertiolate at a concentration of 0.01% and sulema at a concentration of 0.1% were used as sterilizing agents. The isolation of explants was performed in several periods: the beginning of the growth was in February, active growth was in June, the decline of growth was in August. The studies have shown that the maximum aseptic cultures were obtained when processing strawberry plant material with mercurycontaining sulema preparation in the concentration of 0.1%. At the first stage of micropropagation, explants had a high viability during all periods of the isolation, the average survival rate for varieties was 74.8-80.7%. A significant influence of the genotype (varietal characteristics) on the survival rates of explants was noted. The number of explants suitable for cloning did not depend on the overall level of regeneration. Stabilization of the crop during winter introduction was much faster than in other periods. Using the winter term of the isolation of strawberry explants allowed to increase the yield of explants capable of further cloning, accelerate the stabilization of the culture in vitro and reduce the time for obtaining micro-plants suitable for planting in non-sterile conditions. On average, 75.2% of explants capable of further cloning for the varieties were obtained. As a result of the research, the conditions and methods for obtaining the largest number of viable sterile strawberry explants were worked out, which will be included into the process of reproduction in vitro and further research.

In vitro rooting cultivars of black currant (Ribes nigrum L.) of the Bashkir breeding

Biomics ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 332-335 ◽  
Author(s):  
L.A. Golovina ◽  
M.M. Ishmuratova
Keyword(s):  
In Vitro Rooting ◽  
Ribes Nigrum ◽  
Black Currant

scholarly journals The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures

2011 ◽  
Vol 38 (No. 2) ◽  
pp. 49-53 ◽  
Author(s):  
A. Hauptmanová ◽  
J. Polák
Keyword(s):  
In Vitro Cultures ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
Virus Elimination ◽  
Skoog Medium ◽  
Negative Results ◽  
Murashige Skoog ◽  
One Year

In vitro cultures of plum cv. Bluefree and apricot cv. Hanita infected with Plum pox virus (PPV) were used for the virus elimination by chemotherapy. Low ribavirin concentrations of 5 and 10 mg/l in Murashige-Skoog medium were applied in the treatment. Plum pox virus was completely eliminated by 5 mg/l of ribavirin in plum cv. Bluefree within twenty weeks and in apricot cv. Hanita in twelve weeks of the application. Plum pox virus was completely eliminated by 10 mg/l of ribavirin both in plum cv. Bluefree and apricot cv. Hanita within twelve weeks. The presence of PPV was not proved by RT-PCR. Clones of plum cv. Bluefree and apricot cv. Hanita were re-tested by RT-PCR one year after the termination of the ribavirin treatment and negative results confirmed the elimination of Plum pox virus.

scholarly journals In Vitro Regeneration of The Endangered Cactus Turbincarpus Mombergeri Riha, A Hybrid of T. Laui x T. Pseudopectinatus

2021 ◽  
Author(s):  
Maria del Socorro Santos-Diaz ◽  
Ma. Lourdes Santos-Díaz ◽  
Juana Alvarado-Rodríguez
Keyword(s):  
Survival Rate ◽  
Indoleacetic Acid ◽  
In Vitro Regeneration ◽  
Salt Medium ◽  
Natural Conditions ◽  
Normal Morphology ◽  
Culture Techniques ◽  
Seed Disinfection ◽  
Murashige Skoog

Abstract Turbinicarpus mombergeri is a cacti species formed by a hybridization process between Turbinicarpus laui and Turbinicarpus pseudopectinatus. Under natural conditions, it is very difficult for two species be genetically compatible for hybridization, and to produce flowers at the same time. Thus, T. mombergeri is a very interesting and a rare species. Unfortunately, the current populations are decreasing and now it is considered critically endangered. The aim of this research was to develop a successful protocol for propagating T. mombergeri using the in vitro culture techniques. Seed disinfection was performed with Plant Preservative Mixture, and 80% of germination occurred at day 45 in Murashige-Skoog medium. The shoots were cut longitudinally, and the segments were transferred to media containing 2.22 or 4.44 µM benzyladenine to induce shooting. The generated shoots were highly hydrated, and presented abundant callus. The hyperhydricity was controlled by reducing salt medium concentration, by increasing calcium levels and by using polyethylenglycol. The reduction of callus was attained by adding tri-iodo benzoic acid. Vigorous and thick shoots were generated in medium containing urea, and rooting improved in the presence of 0.5 µM indoleacetic acid. Plantlets with normal morphology were obtained, and the survival rate of the plants in soil was 80%. The methodology developed represents an alternative for propagation of T. mombergeri under controlled conditions for commercial or conservation purposes.

scholarly journals Effect of aqueous extracts of aspen, black currant, folded blackberry and walnut leaves on development of pathogenic fungi

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 623-625
Author(s):  
E. Stompor-Chrzan
Keyword(s):  
Juglans Regia ◽  
Fusarium Culmorum ◽  
Aqueous Extracts ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Inhibiting Effect ◽  
Walnut Leaves

The antifungal activity of water extracts of dried leaves of folded blackberry (Rubus plicatus W. et N.), walnut (Juglans regia L.), black currant (Ribes nigrum L.), and aspen (Populus tremula L.) was assessed in the in vitro experiment. The tested fungi were Alternaria alternata, Botrytis cinerea, Rhizoctonia solani, Fusarium culmorum, F. oxysporum and F. solani. It was found that all tested extracts inhibited the development of A. alternaria and F. oxysporum, whereas the strongest inhibiting effect was exhibited by extracts of aspen (A. alternata and F. oxysporum), black current (A. alternata) and folded blackberry (F. oxysporum). The tested extracts had no effect on B. cinerea.

scholarly journals Cryopreservation of shoot tips of endangered Hayachine-usuyukiso ( Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol

2008 ◽  
Vol 6 (02) ◽  
pp. 164-166 ◽  
Author(s):  
Daisuke Tanaka ◽  
Takao Niino ◽  
Yoshiko Tsuchiya ◽  
Kazuto Shirata ◽  
Matsuo Uemura
Keyword(s):  
Survival Rate ◽  
Liquid Nitrogen ◽  
Shoot Tips ◽  
Endangered Plant ◽  
Alpine Plant ◽  
Promising Technique ◽  
Skoog Medium ◽  
Murashige Skoog ◽  
Iwate Prefecture

Hayachine-usuyukiso (Leontopodium hayachinense) is an alpine plant native to Mt Hayachine. This unique chrysanthemum is listed as an endangered plant by the Department of Conservation, Iwate Prefecture, and as a threatened plant by the Ministry of the Environment, Japan. We successfully cryopreserved the shoot tips fromin vitro-grownL. hayachinenseshoots using a vitrification protocol. Cold-hardened shoot tips were excised and pre-cultured on a solidified Murashige–Skoog medium containing 0.3 M sucrose for 1 d at 5°C. The shoot tips were then treated with loading solution for 20 min at 25°C, dehydrated in plant vitrification solution 2 for 120 min at 25°C and immersed in liquid nitrogen. The survival rate of the vitrified shoot tips was 63.3% after 30 d of regrowth. This protocol appears to be a promising technique for the cryopreservation ofin vitro-grown shoots of this endangered plant.

scholarly journals Clonal propagation of black currant (Ribes nigrum L.)

2020 ◽  
Vol 19 (2) ◽  
pp. 005-010
Author(s):  
K. Yu. Guseva
Keyword(s):  
Gibberellic Acid ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Rooting Medium

At the stage of transfer to soil, a scheme is defined that provides production of improved plantlets of thestudied varieties. 'Kuharsky's Memory' and 'Kanakhama' black сurrant varieties are characterized by high regenerativecapacity in vitro (the proportion of viable explants is 93.5 % and 96.4 %, respectively). At the multiplication stage, the influence of growth regulators (6-benzylaminopurine, gibberellic acid) on the morphogenesis of currant plantlets was studied. It shows their impact on such morphological indicators of development of plantlets as the height (cm), quantity, theaverage length of roots (cm). At the rooting stage, the concentration (0.5 mg/l) of auxin (β-indolylbutyric acid) was selected, the introduction of which into the rooting medium provided the maximum indicators of rhizogenesis: the numberand length of roots.

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