Characterisation and functional analysis of novel Scabies Mite Inactivated Cysteine Protease Paralogs (SMIPP-Cs) and establishment of RNA interference in Sarcoptes scabiei

Background: Scabies is a skin disease caused by Sarcoptes scabiei. Scabies is transmitted through direct contact with animals affected by scabies or contact with the source of scabies mite in area of cat lives. Purpose: This study aims to determine the management of scabies at Q-One PetKlinik Surabaya. Method: Handling domestic cats affected by S.scabiei began with an interview with the owner and examinated with the physical condition of cats, and observed the body that was infected with S.scabiei. Scrabbing on scabs was conducted in the cat’s body which was located in the facial area, and followed by microscopic examination to observe the S.scabiei mite. Results: During April 2021, there were 13 domestic cats affected by scabies. Treatment for scabies was by administrated drugs containing 5% Permethrin. Cats were also given supportive therapy by grooming with antiectoparasite shampoo after two weeks from being given drugs. Conclusion: Handling cases of scabies in domestic cats at Q-One PetKlinik Surabaya is performed by cleaning the scab, applying an ointment containing 5% permethrin, and giving anti-histamine and anti-parasitic as well as providing supportive therapy in the form of grooming using shampoo containing anti-ectoparasites.

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Functional Analysis of RNA Interference-Related Soybean Pod Borer (Lepidoptera) Genes Based on Transcriptome Sequences

Frontiers in Physiology ◽

10.3389/fphys.2018.00383 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 4

Author(s):

Fanli Meng ◽

Mingyu Yang ◽

Yang Li ◽

Tianyu Li ◽

Xinxin Liu ◽

...

Keyword(s):

Functional Analysis ◽

Rna Interference ◽

Pod Borer ◽

Transcriptome Sequences

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Functional analysis of cholesterol biosynthesis by RNA interference

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/j.jsbmb.2007.03.001 ◽

2007 ◽

Vol 104 (3-5) ◽

pp. 105-109 ◽

Cited By ~ 6

Author(s):

Christina Guggenberger ◽

Denise Ilgen ◽

Jerzy Adamski

Keyword(s):

Functional Analysis ◽

Rna Interference ◽

Cholesterol Biosynthesis

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Structure-Function of Falcipains: Malarial Cysteine Proteases

Journal of Tropical Medicine ◽

10.1155/2012/345195 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 17

Author(s):

Kailash C. Pandey ◽

Rajnikant Dixit

Keyword(s):

Functional Analysis ◽

Drug Development ◽

Protease Inhibitors ◽

Cysteine Protease ◽

Essential Role ◽

Cysteine Proteases ◽

Structural Studies ◽

Cysteine Protease Inhibitors ◽

Hemoglobin Binding ◽

Structural Insight

Evidence indicates that cysteine proteases play essential role in malaria parasites; therefore an obvious area of investigation is the inhibition of these enzymes to treat malaria. Studies with cysteine protease inhibitors and manipulating cysteine proteases genes have suggested a role for cysteine proteases in hemoglobin hydrolysis. The best characterizedPlasmodiumcysteine proteases are falcipains, which are papain family enzymes. Falcipain-2 and falcipain-3 are major hemoglobinases ofP. falciparum. Structural and functional analysis of falcipains showed that they have unique domains including a refolding domain and a hemoglobin binding domain. Overall, the complexes of falcipain-2 and falcipain-3 with small and macromolecular inhibitors provide structural insight to facilitate the design or modification of effective drug treatment against malaria. Drug development targeting falcipains should be aided by a strong foundation of biochemical and structural studies.

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