Editorial: Injecting new life into intracellular delivery methods

2018 ◽  
Author(s):  
Kathy Myers Gschweng
Keyword(s):  
Intracellular Delivery ◽  
Delivery Methods

scholarly journals Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 631
Author(s):  
Kiran Kaladharan ◽  
Ashish Kumar ◽  
Pallavi Gupta ◽  
Kavitha Illath ◽  
Tuhin Subhra Santra ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Biology ◽  
Transfection Efficiency ◽  
Intracellular Delivery ◽  
Diagnostic Tools ◽  
Delivery Methods ◽  
Physical Methods ◽  
Cell Measurement ◽  
Therapeutic Development ◽  
Single Living

The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics, and drug delivery towards personalized medicine. Various physical delivery methods have long demonstrated the ability to deliver cargo molecules directly to the cytoplasm or nucleus and the mechanisms underlying most of the approaches have been extensively investigated. However, most of these techniques are bulk approaches that are cell-specific and have low throughput delivery. In comparison to bulk measurements, single-cell measurement technologies can provide a better understanding of the interactions among molecules, organelles, cells, and the microenvironment, which can aid in the development of therapeutics and diagnostic tools. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great interest. In recent years, single-cell technologies have become increasingly robust and accessible, although limitations exist. This review article aims to cover various microfluidic-based physical methods for single-cell intracellular delivery such as electroporation, mechanoporation, microinjection, sonoporation, optoporation, magnetoporation, and thermoporation and their analysis. The mechanisms of various physical methods, their applications, limitations, and prospects are also elaborated.

Effectiveness of Three Training Delivery Methods in a Voluntary Program

2009 ◽  
Author(s):  
Arash Salehi ◽  
Lesley Strawderman ◽  
Yunchen Huang ◽  
Shaheen Ahmed ◽  
Kari Babski-Reeves
Keyword(s):  
Delivery Methods ◽  
Voluntary Program ◽  
Training Delivery

Integration of Alternative Technical Concepts with Innovative Delivery Methods

2020 ◽  
Author(s):  
Yifeng Tian ◽  
R. Edward Minchin ◽  
Yuanxin Zhang ◽  
Congwen Kan
Keyword(s):  
Delivery Methods

Diverse Delivery Methods

2017 ◽  
Author(s):  
Julia Benson
Keyword(s):  
Delivery Methods

Probing Cellular Outcomes Using Heterobivalent Constructs

2019 ◽  
Author(s):  
Rohit Bhadoria ◽  
Kefeng Ping ◽  
Christer Lohk ◽  
Ivar Järving ◽  
Pavel Starkov
Keyword(s):  
Cell Viability ◽  
Small Molecules ◽  
Cellular Uptake ◽  
Kinase Inhibitors ◽  
Rho Kinase ◽  
Intracellular Delivery ◽  
Rho Kinase Inhibitors

<div> <div> <div> <p>Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes. </p> </div> </div> </div>

A Mixed Method Investigation of Parent Perspectives on Behavioral Services in Primary Care

2020 ◽  
Author(s):  
Andrew Riley ◽  
Bethany L. Walker ◽  
Krishnapriya Ramanujam ◽  
Wendy M. Gaultney ◽  
Deborah J. Cohen
Keyword(s):  
Primary Care ◽  
Mixed Method ◽  
Behavioral Interventions ◽  
Parental Participation ◽  
Parent Perspectives ◽  
Delivery Methods ◽  
Behavioral Services

Primary care is a key setting for the delivery of parent-focused behavioral interventions. Various methods of intervention show promising efficacy, but fail to engage adequate parental participation. The objective of this study was to understand factors underlying parents’ attitudes towards the content, sources, and delivery methods of behavioral guidance in primary care.

Recent Advances in Novasome Formulation Technology

2014 ◽  
Vol 7 (2) ◽  
pp. 2407-2411
Author(s):  
J M Shah ◽  
N.H Shah ◽  
Hadiya P D
Keyword(s):  
Drug Delivery ◽  
Effective Means ◽  
Entrapment Efficiency ◽  
Bilayer Membrane ◽  
Water Soluble ◽  
Delivery Methods ◽  
Liposomal Drug ◽  
Pharmaceutical Technology ◽  
Insoluble Drugs ◽  
Novel Drug

Pharmaceutical technology has developed various newer modes of novel drug delivery aspects. Modifications in the previously existing drug delivery methods have led to various newly innovated technologies serving as a safe and effective means of improvement over the existing ones. Novasome technology is one of the new innovations of liposomes which have solved many of the problems related to liposomal drug delivery system. It offers a seven bilayer membrane which has the ability to incorporate both water soluble and insoluble drugs. It has an excellent entrapment efficiency which provides better medication. Formulation of novasomes is achieved in a high shear device. Due to its numerous advantages, novasomes have been used extensively in various fields like cosmetics, chemical, personal care, foods, pharmaceuticals and agrochemicals.

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