scholarly journals Determination of Urinary Gluten Immunogenic Peptides to Assess Adherence to the Gluten-Free Diet: A Randomized, Double-Blind, Controlled Study

2021 ◽  
Vol 12 (10) ◽  
pp. e00411
Author(s):  
Chiara Monachesi ◽  
Anil K. Verma ◽  
Giulia N. Catassi ◽  
Elisa Franceschini ◽  
Simona Gatti ◽  
...  
Keyword(s):  
Gluten Free Diet ◽  
Controlled Study ◽  
Gluten Free ◽  
Double Blind ◽  
Immunogenic Peptides

scholarly journals Descriptive Study of the Different Tools Used to Evaluate the Adherence to a Gluten-Free Diet in Celiac Disease Patients

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1777 ◽  
Author(s):  
Luis Rodrigo ◽  
Isabel Pérez-Martinez ◽  
Eugenia Lauret-Braña ◽  
Adolfo Suárez-González
Keyword(s):  
Celiac Disease ◽  
Multidisciplinary Approach ◽  
Descriptive Study ◽  
Duodenal Mucosa ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Autoimmune Process ◽  
Duodenal Biopsies

Celiac disease (CD) is a genetically conditioned autoimmune process that appears in susceptible people. It can affect people of any age, and slightly predominates in females. It has a fairly homogenous global distribution, with an average prevalence of 1–2%, the frequency having increased in recent decades. The only effective treatment is a strict and permanent gluten-free diet (GFD), although the level of compliance is poor, at about 50% of cases. To monitor the effectiveness of the GFD, several procedures involving various approaches are employed: (a) Periodic visits by expert Nutritionists; (b) Clinical follow-up; (c) Serological time controls of specific antibodies; (d) Serial endoscopies with collection of duodenal biopsies; (e) Use of structured questionnaires; and (f) Determination of gluten peptides derived from gluten in faeces and/or urine. All of these procedures are useful when applied, alone or in combination, depending on the cases. Some patients will only need to consult to their doctors, while others will require a multidisciplinary approach to assess their compliance with the GFD. In children, normalization of duodenal mucosa was achieved in 95% of cases within two years, while it is more delayed in adults, whose mucosa take longer time (3–5 years) to heal completely.

Detection of Gluten Immunogenic Peptides in Feces and Urine in Patients with Celiac Disease on a Gluten-Free Diet

2017 ◽  
Vol 152 (5) ◽  
pp. S163
Author(s):  
Emilia Sugai ◽  
Ana Costa ◽  
Ma. de la Paz Temprano ◽  
Sonia I. Niveloni ◽  
Edgardo Smecuol ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Immunogenic Peptides

Mo1043 - Determination of Gluten Consumption by Celiac Disease Patients on a Gluten-Free Diet

2018 ◽  
Vol 154 (6) ◽  
pp. S-681-S-682
Author(s):  
Jennifer A. Sealey Voyksner ◽  
Jack A. Syage ◽  
Matthew A. Dickason ◽  
Ciaran P. Kelly ◽  
Francisco Leon ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Gluten Free Diet ◽  
Gluten Free

scholarly journals Evaluation of Efficacy and Adherence to Gluten-Free Diet in Patients with Celiac Disease

2018 ◽  
Author(s):  
Luis Rodrigo ◽  
Isabel Perez-Martinez ◽  
Eugenia Lauret-Braña ◽  
Adolfo Suarez-Gonzalez
Keyword(s):  
Celiac Disease ◽  
Multidisciplinary Approach ◽  
Duodenal Mucosa ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Autoimmune Process ◽  
Specific Antibodies ◽  
Duodenal Biopsies

Celiac disease (CD) is a genetically conditioned autoimmune process that appears in susceptible people. It can affect people of any age, and slightly predominates in females. It has a fairly homogenous global distribution, with an average prevalence of 1-2%, the frequency having increased in recent decades. The only effective treatment is a strict and permanent gluten-free diet (GFD), although the level of compliance with it is poor, at about 50% of cases. To monitor the effectiveness of the GFD, several procedures involving various approaches are employed: a) periodic interviews by nutritionists; b) clinical follow-up; c) serological controls of specific antibodies; d) endoscopies with collection of duodenal biopsies; e) structured questionnaires; f) determination of gluten peptides derived from gluten in feces and/or urine. All of these procedures are useful when applied, alone or in combination, depending on the cases. Some patients will only need to consult to their doctors, while others will require a multidisciplinary approach to assess their compliance with the GFD. In children, normalization of duodenal mucosa was achieved in 95% of cases within 2 years, while it is more delayed in adults, whose mucosa take longer to heal completely.

scholarly journals Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake

2022 ◽  
Author(s):  
Laura Coto ◽  
Carolina Sousa ◽  
Angel Cebolla
Keyword(s):  
Interindividual Variability ◽  
Elisa Test ◽  
Individual Variability ◽  
High Variability ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Gluten Free ◽  
Immunogenic Peptides ◽  
Stool Samples

Abstract Purpose Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogeneously controlled dietary conditions, and the sensitivity of fecal GIP tests after low amounts of punctual gluten ingestions. Methods Participants (n = 20) followed the same gluten-free menu for 12 days in which two separated doses of gluten (50 mg and 2 g) were ingested and all the depositions were collected. GIP from stool samples were analyzed by ELISA and lateral flow immunoassay (LFIA) tests. Results Most participants had detectable GIP after 50 mg and 2 g gluten ingestions using ELISA test (72.2% and 95%, respectively), whereas the LFIA test showed less sensitivity (22.2% and 80%, respectively). GIP were detected at higher either frequency or concentration in the range of 12–36 h after 50 mg intake, and 12–84 h after 2 g consumption. Considering this period, diagnostic sensitivity of GIP detection after a single 50 mg ingestion may be significatively increased analyzing three stool samples per individual. High variability among participants was found in the time and amount of GIP excretion; however, some individuals showed common patterns for both gluten intakes. Conclusion Sporadic gluten exposure detection may require several fecal samples to achieve level of sensitivity above 90%. Interindividual variability in the dynamic of GIP excretion may suggest patterns of gluten metabolism.

Gluten-related immunogenic peptides in stool as means to monitor compliance with gluten-free diet in children with newly dia gnosed coeliac disease

2021 ◽  
Vol 75 (6) ◽  
pp. 519-523
Author(s):  
Radim Vyhnánek ◽  
Ziad Khaznadar ◽  
Roman Vyhnánek ◽  
Milan Paulík
Keyword(s):  
Coeliac Disease ◽  
Blood Sample ◽  
Stool Sample ◽  
Tissue Transglutaminase ◽  
Sandwich Elisa ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Newly Diagnosed ◽  
Strict Adherence ◽  
Immunogenic Peptides

Objectives and study: To compare the values of gluten-related immunogenic peptides (GIP) in stool and anti-tissue transglutaminase IgA antibodies (anti-tTG IgA) in blood in children newly diagnosed with coeliac disease (CD). Methods: All children (2–15 y) newly diagnosed with CD between May 2018 and May 2020 at our clinic who complied with the inclusion criteria were invited to join the prospective study. During workup for CD, a stool sample to measure GIP was taken together with a blood sample to measure anti-tTG IgA. All newly diagnosed children were invited 4 months later for a check-up. Children and their caregivers were asked about known non-compliance with the gluten-free diet (GFD), a blood sample was taken to measure the anti-tTG IgA, and a stool sample was collected to measure GIP. Blood was evaluated for anti-tTG IgA by ELISA, and the stool was tested by quantitative Sandwich ELISA designed to detect and quantify GIP using the G12 antibody. Values of GIP and anti-tTG IgA were compared in terms of their relation to the upper limit of normal (ULN) of the particular method. Results: 29 children (18 girls) were enrolled in the study. The values of GIP in stool at the time of diagnosis were above the ULN (0.15 µg/g) in all children. Average 4.21, median 3.29, standard deviation (SD) 3.7. After the four months, all but three (89.7%) had values of GIP in the reference range. Average 0.29, median 0.12, SD 0.73. Similarly, anti-tTG IgA values were above the ULN (9.9 U/mL) at the time of diagnosis in all children. Average 164, median 195, SD 49. Although the anti-tTG IgA levels were lower at check-up in all but one child, only 10 (34.5%) showed values within the normal range, with an average of 27.9, median 12.0, and SD 38.9. All children declared strict adherence to GFD. Discussion: Using the GIP concentration in stool, adherence to GFD in our cohort of children is very good, better than that described in literature. Conclusion: Measuring GIP in stool could prove a more sensitive indicator of adherence to GFD in the early months after the diagnosis of CD when anti-tTG IgA are still elevated above the ULN due to their well-described gradual decrease after GFD initiation.

Detection of gluten immunogenic peptides and the Celiac Disease Adherence Test to monitor gluten-free diet: a pilot study

2022 ◽  
Author(s):  
Gry Irene Skodje ◽  
Frida van Megen ◽  
Marianne Stendahl ◽  
Christine Henriksen ◽  
Knut Erik Aslaksen Lundin ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Pilot Study ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Immunogenic Peptides ◽  
Adherence Test

scholarly journals The Pros and Cons of Using Oat in a Gluten-Free Diet for Celiac Patients

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2345 ◽  
Author(s):  
Iva Hoffmanová ◽  
Daniel Sánchez ◽  
Adéla Szczepanková ◽  
Helena Tlaskalová-Hogenová
Keyword(s):  
Essential Amino Acids ◽  
Biologically Active ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Beta Glucans ◽  
Nutritional Qualities ◽  
Long Time ◽  
Pros And Cons ◽  
Safe Dose

A therapeutic gluten-free diet often has nutritional limitations. Nutritional qualities such as high protein content, the presence of biologically active and beneficial substances (fiber, beta-glucans, polyunsaturated fatty acids, essential amino acids, antioxidants, vitamins, and minerals), and tolerance by the majority of celiac patients make oat popular for use in gluten-free diet. The health risk of long-time consumption of oat by celiac patients is a matter of debate. The introduction of oat into the diet is only recommended for celiac patients in remission. Furthermore, not every variety of oat is also appropriate for a gluten-free diet. The risk of sensitization and an adverse immunologically mediated reaction is a real threat in some celiac patients. Several unsolved issues still exist which include the following: (1) determination of the susceptibility markers for the subgroup of celiac patients who are at risk because they do not tolerate dietary oat, (2) identification of suitable varieties of oat and estimating the safe dose of oat for the diet, and (3) optimization of methods for detecting the gliadin contamination in raw oat used in a gluten-free diet.

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