scholarly journals Karakteristik Darah Mimi (Limulus sp) sebagai Pendeteksi Bakteri Kontaminan Penghasil Endotoksin pada Produk Perikanan

2018 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Romadhon Romadhon ◽  
Selamet Suharto ◽  
Sumardianto Sumardianto

Kepiting tapal kuda atau mimi lan mintuno atau belangkas (suku Limulidae) mencakup empat jenis hewan beruas (Artropoda) yang menghuni perairan dangkal wilayah payau dan kawasan mangrove. Mimi merupakan salah satu sumberdaya genetika yang dilindungi. Mimi juga memiliki karakteristik tersendiri dalam darahnya. Umumnya hewan darat maupun laut memiliki darah berwarna merah. Namun, darah pada mimi berwarna biru. Pada Limulus polyphemus memiliki ekstrak darah Limulus Amebosit Lysate (LAL). Tujuan memisahkan dan mengkarakterisasi darah limulus dan menskrining hasil pemisahan darah dengan bakteri patogen. Hasil Penelitian 10 ekor Limulus sp yaitu 165,72 ml. Setelah disentrifuse dihasilkan supernatant  90,633 ml dan  23,304 pelet.Hasil analisis fitokimia banyak dihasilkan pada supernatan terutama alkaloid, fenol, tannin, alkaloid. Berdasarkan skrining dengan bakteri patogen tidak dihasilkan zona hambat karena pengenceran terlalu kecil. Horseshoe crab or mimi lan mintuno or trim (Limulidae tribe) includes four types of extending animals (Artropoda) that inhabit the shallow waters of the brackish area and the mangrove area. Mimi is one of the protected genetic resources. Mimi also has its own characteristics in the blood. Generally land and sea animals have red blood. However, the blood on the mimi is blue. In Limulus polyphemus has Limulus Amebosit Lysate blood extract (LAL). The purpose of separating and characterizing blood limulus and screening the results of blood separation with pathogenic bacteria. Research result of 10 tail Limulus sp that is 165,72 ml. After centrifugation, the supernatant produced 90,633 ml and 23,304 pellets. The result of phytochemical analysis is mostly produced on supernatant especially alkaloid, phenol, tannin, alkaloid. Screening with pathogenic bacteria does not result in inhibit zone because dilution is too small. 

Author(s):  
T. Wichertjes ◽  
E.J. Kwak ◽  
E.F.J. Van Bruggen

Hemocyanin of the horseshoe crab (Limulus polyphemus) has been studied in nany ways. Recently the structure, dissociation and reassembly was studied using electron microscopy of negatively stained specimens as the method of investigation. Crystallization of the protein proved to be possible and X-ray crystallographic analysis was started. Also fluorescence properties of the hemocyanin after dialysis against Tris-glycine buffer + 0.01 M EDTA pH 8.9 (so called “stripped” hemocyanin) and its fractions II and V were studied, as well as functional properties of the fractions by NMR. Finally the temperature-jump method was used for assaying the oxygen binding of the dissociating molecule and of preparations of isolated subunits. Nevertheless very little is known about the structure of the intact molecule. Schutter et al. suggested that the molecule possibly consists of two halves, combined in a staggered way, the halves themselves consisting of four subunits arranged in a square.


2021 ◽  
pp. 1-2
Author(s):  
Philip M. Novack-Gottshall ◽  
Roy E. Plotnick

The horseshoe crab Limulus polyphemus (Linnaeus, 1758) is a famous species, renowned as a ‘living fossil’ (Owen, 1873; Barthel, 1974; Kin and Błażejowski, 2014) for its apparently little-changed morphology for many millions of years. The genus Limulus Müller, 1785 was used by Leach (1819, p. 536) as the basis of a new family Limulidae and synonymized it with Polyphemus Lamarck, 1801 (Lamarck's proposed but later unaccepted replacement for Limulus, as discussed by Van der Hoeven, 1838, p. 8) and Xyphotheca Gronovius, 1764 (later changed to Xiphosura Gronovius, 1764, another junior synonym of Limulus). He also included the valid modern genus Tachypleus Leach, 1819 in the family. The primary authority of Leach (1819) is widely recognized in the neontological literature (e.g., Dunlop et al., 2012; Smith et al., 2017). It is also the authority recognized in the World Register of Marine Species (WoRMS Editorial Board, 2021).


2005 ◽  
Vol 121 (4) ◽  
pp. 365-377 ◽  
Author(s):  
Mary Kimble ◽  
Yvonne Coursey ◽  
Nina Ahmad ◽  
Gertrude W. Hinsch

1987 ◽  
Vol 173 (2) ◽  
pp. 289-298 ◽  
Author(s):  
MARK L. BOTTON ◽  
ROBERT E. LOVELAND

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