scholarly journals Isolation and characterization of Rhizobium sp. from a collar rot tolerant groundnut (Arachis hypogaea L.) variety

2019 ◽  
Vol 6 (sp1) ◽  
pp. 631-634
Author(s):  
Shalvi Pandey ◽  
Dipjyoti Chakraborty
Keyword(s):  
Bacterial Isolate ◽  
Crop Productivity ◽  
Symbiotic Bacteria ◽  
Collar Rot ◽  
Isolation And Characterization ◽  
Arachis Hypogaea L ◽  
Early Seedling ◽  
Rot Disease ◽  
Rhizobium Sp

Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germination and early seedling stage. In the present study, symbiotic bacteria was isolated and characterised from a collar rot resistant groundnut variety, Mallika and identified as Rhizobium pongamiae following biochemical and molecular characterization (NCBI Acc. No. MK770416). The bacterial isolate on cross inoculation, may be useful in improving crop productivity and enhancing collar rot disease tolerance in susceptible crops.

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

A Pseudomonas guariconensis strain capable of promoting growth and controlling collar rot disease in Arachis hypogaea L

2015 ◽  
Vol 390 (1-2) ◽  
pp. 369-381 ◽  
Author(s):  
Ravi R. Patel ◽  
Vasudev R. Thakkar ◽  
Bagavathi R. Subramanian
Keyword(s):  
Arachis Hypogaea ◽  
Collar Rot ◽  
Arachis Hypogaea L ◽  
Rot Disease

Isolation and characterization of fatty acid desaturase genes from peanut (Arachis hypogaea L.)

2011 ◽  
Vol 30 (8) ◽  
pp. 1393-1404 ◽  
Author(s):  
Xiaoyuan Chi ◽  
Qingli Yang ◽  
Lijuan Pan ◽  
Mingna Chen ◽  
Yanan He ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Arachis Hypogaea ◽  
Fatty Acid Desaturase ◽  
Isolation And Characterization ◽  
Arachis Hypogaea L

scholarly journals Isolation and Characterization of Photorhabdus Spp. (Enterobacteriaceae) Isolated From Heterorhabditis Bacteriophora in Kashere, Nigeria

2021 ◽  
Vol 25 (8) ◽  
pp. 1345-1348
Author(s):  
H.U. Aliyu ◽  
F. Tahir ◽  
E.B. Agbo ◽  
S.L. Kela
Keyword(s):  
Biological Control ◽  
Bacterial Isolate ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Heterorhabditis Bacteriophora ◽  
Biochemical Tests ◽  
Susceptible Host ◽  
Isolation And Characterization ◽  
Biological Control Potential

Photorhabdus bacteria are symbiotically associated with the infective juveniles of entomopathogenic nematodes of the genus Heterorhabditis. These bacteria after infecting a susceptible host, produces a host of lethal toxins that killthe insect hosts within 24 to 72 hours. Consequently they have emerged and are trending as excellent biological control agents against insect pests of agricultural crops. Therefore, this study aim to isolate, identify and test the pathogenicity of Photorhabdus bacteria isolated from Heterorhabditis bacteriophora, entomopathogenic nematode from Kashere, Gombe State, Nigeria. To this end phenotypic and biochemical tests were conducted. The tests conducted showed that the isolate exhibited characteristics similar to those of Photorhabdus bacteria that have been identified. Last instar larvae of G. mellonella exposed to different concentrations of the bacterial isolate showed some level of susceptibility of the larvae to the bacterial isolate confirming its biological control potential.

scholarly journals Isolation and Characterization of Pectobacterium Phage vB_PatM_CB7: New Insights into the Genus Certrevirus

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 352
Author(s):  
Colin Buttimer ◽  
Caoimhe Lynch ◽  
Hanne Hendrix ◽  
Horst Neve ◽  
Jean-Paul Noben ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Burst Size ◽  
Homing Endonuclease ◽  
Soft Rot ◽  
Nucleotide Metabolism ◽  
Homing Endonucleases ◽  
Isolation And Characterization ◽  
Rot Disease ◽  
Large Terminase

To date, Certrevirus is one of two genera of bacteriophage (phage), with phages infecting Pectobacterium atrosepticum, an economically important phytopathogen that causes potato blackleg and soft rot disease. This study provides a detailed description of Pectobacterium phage CB7 (vB_PatM_CB7), which specifically infects P. atrosepticum. Host range, morphology, latent period, burst size and stability at different conditions of temperature and pH were examined. Analysis of its genome (142.8 kbp) shows that the phage forms a new species of Certrevirus, sharing sequence similarity with other members, highlighting conservation within the genus. Conserved elements include a putative early promoter like that of the Escherichia coli sigma70 promoter, which was found to be shared with other genus members. A number of dissimilarities were observed, relating to DNA methylation and nucleotide metabolism. Some members do not have homologues of a cytosine methylase and anaerobic nucleotide reductase subunits NrdD and NrdG, respectively. Furthermore, the genome of CB7 contains one of the largest numbers of homing endonucleases described in a single phage genome in the literature to date, with a total of 23 belonging to the HNH and LAGLIDADG families. Analysis by RT-PCR of the HNH homing endonuclease residing within introns of genes for the large terminase, DNA polymerase, ribonucleotide reductase subunits NrdA and NrdB show that they are splicing competent. Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was also performed on the virion of CB7, allowing the identification of 26 structural proteins—20 of which were found to be shared with the type phages of the genera of Vequintavirus and Seunavirus. The results of this study provide greater insights into the phages of the Certrevirus genus as well as the subfamily Vequintavirinae.

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