scholarly journals Transplantation of ex vivo expanded limbal stem cells

2017 ◽  
Vol 61 (2) ◽  
pp. 32
Author(s):  
R. Hristova ◽  
Y. Zdravkov ◽  
I. Tanev
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Limbal Stem Cells

Human Anterior Lens Capsule as a Biologic Substrate for the Ex Vivo Expansion of Limbal Stem Cells in Ocular Surface Reconstruction

Cornea ◽  
2007 ◽  
Vol 26 (4) ◽  
pp. 473-478 ◽  
Author(s):  
Ahmed Galal ◽  
Juan J Perez-Santonja ◽  
Jose Luis Rodriguez-Prats ◽  
Marta Abad ◽  
Jorge Alio
Keyword(s):  
Stem Cells ◽  
Surface Reconstruction ◽  
Ocular Surface ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Lens Capsule ◽  
Limbal Stem Cells ◽  
Ocular Surface Reconstruction ◽  
Anterior Lens Capsule

scholarly journals Ex vivo expansion of limbal stem cells is affected by substrate properties

2012 ◽  
Vol 8 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Roanne R. Jones ◽  
Ian W. Hamley ◽  
Che J. Connon
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Limbal Stem Cells ◽  
Substrate Properties

scholarly journals Limbal stem cell deficiency: etiology, pathogenesis, priniciples and prospects of surgical treatment

2019 ◽  
Vol 12 (1) ◽  
pp. 103-111
Author(s):  
A. S. Dubovikov ◽  
I. O. Gavrilyuk ◽  
A. N. Kulikov ◽  
S. V. Churashov ◽  
V. F. Chernysh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Surgical Treatment ◽  
Ex Vivo ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cells ◽  
History Of Development ◽  
History Of ◽  
Limbal Epithelial Stem Cells ◽  
Limbal Stem Cell

The review is focused on the modern view of the etiology and pathogenesis of limbal stem cells deficiency. The history of development of tissue and ex-vivo transplantation of limbal epithelial stem cells is presented. Certain promising directions of the treatment of patients with limbal stem cells deficiency are presented.

scholarly journals Transplantation of ex vivo expanded limbal stem cells in neurotrophic keratopathy – a case report

2016 ◽  
Vol 60 (4) ◽  
pp. 5
Author(s):  
R. Hristova ◽  
Y. Zdravkov ◽  
M. Hristova ◽  
I. Tanev
Keyword(s):  
Stem Cells ◽  
Case Report ◽  
Ex Vivo ◽  
Limbal Stem Cells ◽  
Neurotrophic Keratopathy

Cryopreservation of Human Limbal Stem Cells Ex Vivo Expanded on Amniotic Membrane

Cornea ◽  
2008 ◽  
Vol 27 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Hui-Jung Yeh ◽  
Chao-Ling Yao ◽  
Hsin-I Chen ◽  
Huey-Chuan Cheng ◽  
Shiaw-Min Hwang
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Ex Vivo ◽  
Limbal Stem Cells

scholarly journals Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Nuria Nieto-Nicolau ◽  
Eva M. Martínez-Conesa ◽  
Ricardo P. Casaroli-Marano
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Quality Criteria ◽  
Corneal Opacity ◽  
Clinical Approach ◽  
Limbal Stem Cells ◽  
Need To Evaluate ◽  
Forming Efficiency ◽  
Cadaveric Donors ◽  
Graft Quality

Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) afterex vivoexpansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor.

Limbal Stem Cells in Health and Disease

2001 ◽  
Vol 21 (4) ◽  
pp. 385-405 ◽  
Author(s):  
Virender S. Sangwan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Ocular Surface ◽  
Ex Vivo ◽  
Human Amniotic Membrane ◽  
Limbal Stem Cells ◽  
Corneal Surface ◽  
Health And Disease ◽  
Cell Phenotypes

Stem cells are present in all self-reviewing tissues and have unique properties. The ocular surface is made up of two distinct types of epithelial cells, constituting the conjunctival and the corneal epithelia. These epithelia are stratified, squamous and non-keratinized. Although anatomically continuous with each other at the corneoscleral limbus, the two cell phenotypes represent quite distinct subpopulations. The stem cells for the cornea are located at the limbus. The microenvironment of the limbus is considered to be important in maintaining stemness of the stem cells. They also act as a “barrier” to conjunctival epithelial cells and prevent them from migrating on to the corneal surface. In certain pathologic conditions, however, the limbal stem cells may be destroyed partially or completely resulting in varying degrees of stem cell deficiency with its characteristic clinical features. These include “conjunctivalization” of the cornea with vascularization, appearance of goblet cells, and an irregular and unstable epithelium. The stem cell deficiency can be managed with auto or allotransplantation of these cells. With the latter option, systemic immunosuppression is required. The stem cells can be expanded ex vivo on a processed human amniotic membrane and transplanted back to ocular surface with stem cell deficiency without the need of immunosuppression.

Autologous Cultivated Limbal Stem Cell Transplantation after Failed Previous Limbal Graft

2017 ◽  
Vol 27 (5) ◽  
pp. e137-e139
Author(s):  
Karl A. Knutsson ◽  
Stanislav Matuska ◽  
Paolo Rama
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Ocular Surface ◽  
Ex Vivo ◽  
Limbal Stem Cells ◽  
Limbal Stem Cell Transplantation ◽  
Limbal Stem Cell ◽  
Contralateral Eye

Purpose To describe a case of unilateral limbal stem cell deficiency (LSCD) with previously failed autologous graft, resolved by ocular surface reconstruction using cultured autologous limbal stem cells from the contralateral eye. Case Report A 35-year-old patient presented to our clinic with LSCD due to a unilateral alkali burn. The patient had received a previous limbal graft from the contralateral eye that had failed to impede corneal conjunctivalization. We decided to repeat limbal stem cell transplantation using an ex vivo cultivation procedure to reduce the risk of tissue harvesting on the healthy fellow eye. A small limbal biopsy (1.5 × 1.5 mm) near the previously excised limbus was performed. Stem cells were then isolated and cultured on fibrin and a 3T3 feeder cell layer using a standard protocol. Four months later, the cultivated cells on fibrin were grafted after pannus removal. In the subsequent months, the ocular surface stabilized and inflammation decreased. Two years later, the patient underwent large tectonic lamellar keratoplasty for severe corneal thinning involving the entire cornea, and 6 months later central penetrating keratoplasty and extracapsular cataract extraction with intraocular lens implantation and pupilloplasty was performed. Following reconstruction, the patient showed improved best-corrected vision from count fingers to 20/200 due to amblyopia, and the ocular surface was stable with a transparent corneal graft. Conclusions Ex vivo limbal stem cell transplantation is a valid technique for treating LSCD and can be utilized for treating patients who have had previous failed limbal grafts.

Unrestricted somatic stem cells, as a novel feeder layer: Ex vivo culture of human limbal stem cells

2017 ◽  
Vol 119 (3) ◽  
pp. 2666-2678 ◽  
Author(s):  
Ebrahim Shirzadeh ◽  
Saeed Heidari Keshel ◽  
Vahid Ezzatizadeh ◽  
Sayena Jabbehdari ◽  
Alireza Baradaran-Rafii
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Feeder Layer ◽  
Somatic Stem Cells ◽  
Limbal Stem Cells ◽  
Ex Vivo Culture ◽  
Unrestricted Somatic Stem Cells
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