scholarly journals Gamma radiation sensitivity in Nigella sativa L.

CYTOLOGIA ◽  
1986 ◽  
Vol 51 (3) ◽  
pp. 609-615 ◽  
Author(s):  
Animesh K. Datta ◽  
Amal K. Biswas ◽  
Subir Sen
1972 ◽  
Vol 104 (10) ◽  
pp. 1551-1556 ◽  
Author(s):  
John H. Brower ◽  
Hilda C. Scott

AbstractThe effects of seven gamma radiation dosages from 5 to 100 krad on all metamorphic stages of the spider beetle, Gibbium psylloides (Czenpinski), were studied. Five kilorads prevented development of adults from treated eggs and larvae. Some adults emerged from the pupal cocoon at all treatment levels except 50 and 100 krad, but their longevity was greatly reduced. Adults were fairly resistant to sterilizing effects of the radiation. Twenty kilokrads was not completely sterilizing to either males or females, but fecundity was greatly reduced. The longevity of treated adults was significantly reduced, but some survived for 20 weeks after a 20-krad exposure. Adults and pupae of this species were more radiation resistant than were most other stored-product Coleoptera.


2006 ◽  
Vol 69 (6) ◽  
pp. 1434-1437 ◽  
Author(s):  
J. W. LEE ◽  
S. H. OH ◽  
J. H. KIM ◽  
H. S. YOOK ◽  
M. W. BYUN

The observed Enterobacter sakazakii D10-values for tryptic soy broth and dehydrated powdered infant formula were 0.27 ± 0.05 and 0.76 ± 0.08 kGy, respectively. A decrease of approximately 3 log in the dehydrated powdered infant formula was obtained by irradiation with 3.0 kGy or rehydration with hot water at 80°C. No recoverable bacteria were found in the powdered infant formula irradiated at 5.0 kGy and stored, either before or after rehydration. A radiation dose of up to 5.0 kGy had no marked effect on the sensory properties of the dehydrated powdered infant formula after rehydration and heating. Gamma radiation could potentially be used to inactivate E. sakazakii in dehydrated powdered infant formula; however, nutritional studies need to be conducted before the use of radiation can be recommended.


1980 ◽  
Vol 187 (1) ◽  
pp. 257-260 ◽  
Author(s):  
E T J Chelton ◽  
M Duggan ◽  
R N Hunston ◽  
A S Jones ◽  
M K O'Leary ◽  
...  

Bacteriophage T3 was produced in a form that contained 32% of its normal DNA thymine residues replaced with 5-vinyluracil residues by infecting a thymine-requiring strain of Escherichia coli with phage T3 in a medium containing 5-vinyluracil. When 2′-deoxy-5-vinyluridine was added to the medium instead, no incorporation was observed into the phage DNA, and the presence of the deoxyribonucleoside severely decreased the number of viable phage particles produced. The analogue-containing phage, although initially viable, rapidly lost viability when stored, but it was no more sensitive than was normal phage T3 to the effect of gamma-radiation.


2010 ◽  
Vol 50 (3) ◽  
pp. 250-255 ◽  
Author(s):  
Abbas Hosseinzadeh ◽  
Nouraddin Shayesteh ◽  
Hamid Zolfagharieh ◽  
Mohammad Babaei ◽  
Hasan Zareshahi ◽  
...  

Gamma Radiation Sensitivity of Different Stages of Saw-Toothed Grain BeetleOryzaephilus SurinamensisL. (Coleoptera: Silvanidae)The effect of gamma irradiation on different developmental stages ofOryzaephilus surinamensisL., was investigated. Results showed that a required dose to prevent larval emergence from irradiated 1-2-days-old eggs was 60 Gray (Gy), and 350 Gy was required to prevent adult emergence from 15-days-old larvae. Also the required dose of radiation to prevent adult emergence from irradiated 5-days-old pupa was 700 Gy. The dose of 200 Gy caused 100% mortality of irradiated adults 28 days after treatment. In addition, the effect of gamma rays was studied on the developmental stage period of each irradiated stage till adult emergence. The results revealed that there was a dose-dependent increase of the developmental periods. The growth index of adults was significantly decreased with increasing dose of radiation administered to eggs, larvae and pupae. It is recommended that doses between 600 and 700 Gy should be used to control population growth ofO. surinamensiswhen targeting pupae and adults present in stored products.


Cancer ◽  
2004 ◽  
Vol 100 (3) ◽  
pp. 561-567 ◽  
Author(s):  
Rong Zheng ◽  
Li-E Wang ◽  
Melissa L. Bondy ◽  
Qingyi Wei ◽  
Erich M. Sturgis

2003 ◽  
Vol 23 (15) ◽  
pp. 5346-5353 ◽  
Author(s):  
Elisabeth Larsen ◽  
Christine Gran ◽  
Barbro Elisabet Sæther ◽  
Erling Seeberg ◽  
Arne Klungland

ABSTRACT Flap endonuclease 1 (FEN1) has been shown to remove 5′ overhanging flap intermediates during base excision repair and to process the 5′ ends of Okazaki fragments during lagging-strand DNA replication in vitro. To assess the in vivo role of the mammalian enzyme in repair and replication, we used a gene-targeting approach to generate mice lacking a functional Fen1 gene. Heterozygote animals appear normal, whereas complete depletion of FEN1 causes early embryonic lethality. Fen1−/− blastocysts fail to form inner cell mass during cellular outgrowth, and a complete inactivation of DNA synthesis in giant cells of blastocyst outgrowth was observed. Exposure of Fen1−/− blastocysts to gamma radiation caused extensive apoptosis, implying an essential role for FEN1 in the repair of radiation-induced DNA damage in vivo. Our data thus provide in vivo evidence for an essential function of FEN1 in DNA repair, as well as in DNA replication.


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