scholarly journals Evaluation of Polymorphism in IGF-I and IGFB-3 Genes and their Relationship tith Twinning Rate and Growth Traits in Markhoz Goats

2017 ◽  
Vol 17 (1) ◽  
pp. 89-103 ◽  
Author(s):  
Sahar Rasouli ◽  
Alireza Abdolmohammadi ◽  
Alireza Zebarjadi ◽  
Ali Mostafaei
Keyword(s):  
Growth Factor ◽  
Growth Traits ◽  
Average Daily Gain ◽  
Insulin Like Growth Factor ◽  
Economic Traits ◽  
Chi Square ◽  
Genotype Frequencies ◽  
Igf I ◽  
I Gene ◽  
Igfbp 3

AbstractGrowth rate and twinning rate are economic traits that can be used in goat breeding objectives. The aim of this study was to investigate polymorphisms in the insulin-like growth factor 1(IGF-I) and insulin-like growth factor binding protein (IGFBP-3) genes and their relationship with growth traits and twinning in Markhoz goats. Two sets of specific primers were used to amplify a 249bp fragment of IGF-I gene and a 316bp fragment of IGFBP-3 gene. PCR-SSCP analysis revealed three banding patterns for each gene that confirmed presence of a mutation in position 1617 of the IGF-I gene and a mutation in position 58 of IGFBP-3 gene. The genotype frequencies of IGF-I gene were 0.81 (GG), 0.16 (GA) and 0.03 (AA). Also, the genotype frequencies of IGFBP-3 gene were 0.79 (TT), 0.17 (TC) and 0.04 (CC). The Odds Ratio estimated for twinning rate was 1.11 for second on first parity, 0.19 for third on first parity and 5.71 for second on third parity. The chi-square statistics were 6.46 for IGF-I gene and 3.32 for IGFBP-3 gene. The results also indicated that different genotypes of these genes had no significant effect on birth weight, weight at 6 months, at 9 months and at 12 months but the interactions between different genotypes of IGF-I and IGFBP-3 genes were significant for weaning weight and average daily gain from birth to weaning. These results suggest that twinning rate in Markhoz breed is statistically affected by these genes and can be considered in breeding programs.

Polymorphism of Insulin-Like Growth Factor I Gene among Chicken Breeds in Egypt

2010 ◽  
Vol 65 (3-4) ◽  
pp. 284-288 ◽  
Author(s):  
Eman M. Gouda ◽  
Gamal S. Essawy
Keyword(s):  
Growth Factor ◽  
Growth Traits ◽  
Sscp Analysis ◽  
Insulin Like Growth Factor ◽  
Rt Pcr ◽  
Factor I ◽  
Igf I ◽  
Pcr Products ◽  
Growth Factor I ◽  
I Gene

The insulin-like growth factor I (IGF-I) regulates growth, protein synthesis, and cell proliferation and differentiation in vertebrates. Polymorphisms of IGF-I gene transcripts of three breeds of chicken were assessed. The associations of these polymorphisms with the growth rate of the studied breeds were also evaluated. Total RNA was isolated from chicken livers, and the IGF-I gene was amplified from each breed RNA by RT-PCR using specific primers flanking a certain region of the gene. The amplified RT-PCR products were formed to identify the transcripts and to correlate them to the phenotype of growth, by performing single stranded conformation polymorphism (SSCP) analysis for genotype identification. In this report, we describe how SSCP analysis of RT-PCR products can be used to evaluate the transcript expression pattern of avian IGF-I polymorphism, and their effect on the growth traits of chickens.

Specimen processing time and measurement of total insulin-like growth factor-I (IGF-I), free IGF-I, and IGF binding protein-3 (IGFBP-3)

2006 ◽  
Vol 16 (2) ◽  
pp. 86-92 ◽  
Author(s):  
Tiffany G. Harris ◽  
Howard D. Strickler ◽  
Herbert Yu ◽  
Michael N. Pollak ◽  
E. Scott Monrad ◽  
...  
Keyword(s):  
Growth Factor ◽  
Processing Time ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Specimen Processing ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Igfbp 3

Growth hormone deficiency in 'little' mice results in aberrant body composition, reduced insulin-like growth factor-I and insulin-like growth factor-binding protein-3 (IGFBP-3), but does not affect IGFBP-2, -1 or -4

1993 ◽  
Vol 136 (1) ◽  
pp. 91-104 ◽  
Author(s):  
L. R. Donahue ◽  
W. G. Beamer
Keyword(s):  
Body Composition ◽  
Growth Factor ◽  
Gh Deficiency ◽  
Body Water ◽  
Insulin Like Growth Factor ◽  
Gh Therapy ◽  
Factor I ◽  
Igf I ◽  
Body Compartments ◽  
Igfbp 3

ABSTRACT Although GH is known to regulate somatic growth during development, its role in regulating adult body composition is less well defined. The effects of GH on individual body compartments – water, fat, protein and mineral – are achieved both by the action of GH and by a GH-induced hormone, insulin-like growth factor-I (IGF-I). We used a genetic model of GH deficiency, the 'little' (gene symbol lit) mouse, to determine the GH regulation of IGF-I and its insulin-like growth factor-binding proteins (IGFBPs) and to define the interaction between these hormones and each body compartment in adults. Our results showed that GH-deficient lit/lit mice had reduced levels of serum IGF-I (range 38–130 μg/l) compared with normal lit/+ littermates (range 432–567 μg/l) between 2 and 52 weeks of age. The lit/lit mice did not experience the fivefold increase in IGF-I between 2 and 4 weeks of age that was seen in lit/+ mice. In lit/lit serum, overall binding of 125I-labelled IGF-I to the four IGFBPs was reduced, solely in response to a reduced amount of IGFBP-3. No overall differences were found between lit/lit and lit/+ mice in the binding of 125I-labelled IGF-I to IGFBP-2, -1 or -4. Age-related declines in IGF-I and IGFBPs were seen in lit/lit mice. However, adult levels of IGF-I were maintained in lit/+ mice to at least 52 weeks of age, as were levels of IGFBP-1 and -4, while IGFBP-3 and -2 declined with age. With respect to body composition, comparison of lit/lit with lit/+ mice showed that the lit/lit mice were characterized by abnormally large adipose tissue stores and reduced body water, protein and mineral from 2 weeks onward. These changes occurred despite normal energy intake in lit/lit mice up to 52 weeks of age, indicating that neither undernutrition nor hyperphagia is characteristic of this GH-induced model of obesity. Furthermore, lit/lit males accrued more body fat beginning at an earlier age than lit/lit females. With advancing age, the per cent body fat increased in both lit/lit and lit/+ mice, while the per cent body water and mineral declined. In lit/lit but not lit/+ mice, per cent protein also declined with age. The changes in body water and fat are attributable to lack of adequate GH in the genetically GH-deficient lit/lit mouse. On the other hand, the changes in body protein are more likely to be effects of IGF-I. Changes in mineral observed in lit/lit mice could be the result of action by GH, IGF-I or both hormones. Therefore, when GH is chronically manipulated by GH deficiency as in lit/lit mice, by GH excess as in acromegaly, or by GH therapy, all four body compartments are affected, suggesting that GH therapy is most valuable when the treatment goal is to alter overall body composition. Journal of Endocrinology (1993) 136, 91–104

scholarly journals Genetic Determinants of Circulating Insulin-Like Growth Factor (IGF)-I, IGF Binding Protein (BP)-1, and IGFBP-3 Levels in a Multiethnic Population

2007 ◽  
Vol 92 (9) ◽  
pp. 3660-3666 ◽  
Author(s):  
Iona Cheng ◽  
Katherine DeLellis Henderson ◽  
Christopher A. Haiman ◽  
Laurence N. Kolonel ◽  
Brian E. Henderson ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Genetic Determinants ◽  
Igf I ◽  
Igf Binding ◽  
Multiethnic Population ◽  
Igf Binding Protein ◽  
Igfbp 3

Regulation and localization of the insulin-like growth factor system in small bowel during altered nutrient status

1995 ◽  
Vol 268 (4) ◽  
pp. G631-G640 ◽  
Author(s):  
D. E. Winesett ◽  
M. H. Ulshen ◽  
E. C. Hoyt ◽  
N. K. Mohapatra ◽  
C. R. Fuller ◽  
...  
Keyword(s):  
Growth Factor ◽  
Small Bowel ◽  
Lamina Propria ◽  
Elemental Diet ◽  
Nutrient Status ◽  
Insulin Like Growth Factor ◽  
Nutrient Regulation ◽  
Igf I ◽  
Ad Libitum ◽  
Igfbp 3

Insulin-like growth factor-I (IGF-I) may regulate small bowel growth. Analyses here in ad libitum-fed, fasted, and refed rats demonstrate that during fasting and refeeding changes in jejunal mass correlate with changes in serum IGF-I and jejunal IGF-I mRNAs. These data indicate that circulating and locally expressed IGF-I contribute to nutrient regulation of jejunal mass. During refeeding, jejunal IGF binding protein 3 (IGFBP-3) mRNA abundance was reduced relative to that of IGF-I, possibly amplifying enterotrophic actions of IGF-I. Localization of IGFBP-3 to subepithelial cells in lamina propria of jejunum indicates that IGFBP-3 derived from lamina propria may modulate IGF-I action on adjacent epithelium. Ileum differed from jejunum in that refeeding did not increase bowel mass or IGF-I mRNA to ad libitum values. Differences in exposure to luminal nutrient may underlie distinct responses of the two segments. Rats fed elemental diet intravenously showed reduced jejunal mass but not reduced jejunal IGF-I mRNA compared with rats fed oral elemental diet. Elemental nutrient given intravenously or orally therefore does not differ in effects on jejunal IGF-I expression. Complex luminal nutrient may, however, regulate jejunal IGF-I expression.

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