Single-shot phase-measuring deflectometry for cornea measurement

2016 ◽  
Vol 5 (5-6) ◽  
Author(s):  
Hanning Liang ◽  
Evelyn Olesch ◽  
Zheng Yang ◽  
Gerd Häusler

AbstractPhase-measuring deflectometry (PMD) has become a standard tool to measure the topography of specular surfaces. We implemented PMD for the measurement of the human cornea topography, exploiting an earlier idea of Lingelbach et al. Two problems occur: a large angular dynamical range and a single-shot measurement are required. We solve these problems by an optimized geometry with minimal occlusion and by single sideband demodulation with a pre-distorted fringe pattern with optimal fringe period. An

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Parsa Omidi ◽  
Mohamadreza Najiminaini ◽  
Mamadou Diop ◽  
Jeffrey J. L. Carson

AbstractSpatial resolution in three-dimensional fringe projection profilometry is determined in large part by the number and spacing of fringes projected onto an object. Due to the intensity-based nature of fringe projection profilometry, fringe patterns must be generated in succession, which is time-consuming. As a result, the surface features of highly dynamic objects are difficult to measure. Here, we introduce multispectral fringe projection profilometry, a novel method that utilizes multispectral illumination to project a multispectral fringe pattern onto an object combined with a multispectral camera to detect the deformation of the fringe patterns due to the object. The multispectral camera enables the detection of 8 unique monochrome fringe patterns representing 4 distinct directions in a single snapshot. Furthermore, for each direction, the camera detects two π-phase shifted fringe patterns. Each pair of fringe patterns can be differenced to generate a differential fringe pattern that corrects for illumination offsets and mitigates the effects of glare from highly reflective surfaces. The new multispectral method solves many practical problems related to conventional fringe projection profilometry and doubles the effective spatial resolution. The method is suitable for high-quality fast 3D profilometry at video frame rates.


2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Kyrollos Yanny ◽  
Nick Antipa ◽  
William Liberti ◽  
Sam Dehaeck ◽  
Kristina Monakhova ◽  
...  

Abstract Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture stop. Placing the phase mask at the aperture stop significantly reduces the size of the device, and varying the focal lengths enables a uniform resolution across a wide depth range. The phase mask encodes the 3D fluorescence intensity into a single 2D measurement, and the 3D volume is recovered by solving a sparsity-constrained inverse problem. We provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the field-varying aberrations in miniature objectives. We demonstrate a prototype that is 17 mm tall and weighs 2.5 grams, achieving 2.76 μm lateral, and 15 μm axial resolution across most of the 900 × 700 × 390 μm3 volume at 40 volumes per second. The performance is validated experimentally on resolution targets, dynamic biological samples, and mouse brain tissue. Compared with existing miniature single-shot volume-capture implementations, our system is smaller and lighter and achieves a more than 2× better lateral and axial resolution throughout a 10× larger usable depth range. Our microscope design provides single-shot 3D imaging for applications where a compact platform matters, such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.


Photonics ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 178
Author(s):  
Bingquan Chen ◽  
Hongxiu Gao ◽  
Hongsheng Li ◽  
Hongyang Ma ◽  
Peng Gao ◽  
...  

In this study, both theoretical analysis and experimental validation are carried out for 3D surface measurement under different indoor/outdoor environmental conditions via combining the projected laser-beam-based sinusoidal optical signal, the optical filtering technique, and the single-shot approach based on Fourier transform profilometry. The designed optical signal generator used in this work is capable of ensuring that the projected fringe pattern is monochromatic, higher-contrast, time-invariant, and truly sinusoidal. The proposed and developed optical setup of 3D surface measurement is portable and is used for in-situ experiments of 3D surface measurements that have been carried out under different sunlight illuminations. The experimental results indicate that accurate reconstructions of measured objects with even or varying surface reflectivity can be obtained under windy conditions and strong environmental illuminations such as the background illuminance of 5600–35,000 Lux. The generated fringe-pattern signal is not sensitive to vibrations from environmental influences including the effects of the wind, which has overcome the outdoor-measurement restrictions of the traditional interferometric system and the profilometry approaches based on phase-shifting methods.


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