Molecular characterization and phylogeny of some mazocraeidean monogeneans from carangid fish

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Neeraja Tambireddy ◽  
Tripathi Gayatri ◽  
Pathakota Gireesh-Babu ◽  
Annam Pavan-Kumar
Keyword(s):  
Bayesian Inference ◽  
Phylogenetic Reconstruction ◽  
Dna Barcodes ◽  
Rdna Sequences ◽  
28S Rdna Sequences ◽  
High Bootstrap ◽  
Coi Sequences ◽  
Inference Methods ◽  
Host Parasite ◽  
First Time

AbstractPolyopisthocotylean monogenean parasites of fishes are highly host specific and have been used as an appropriate model to study the host-parasite co-evolution. In the present study, eight monogeneans of the order Mazocraeidea were characterized by nuclear 28S rDNA sequences and their phylogenetic relationship with other polyopisthocotylean species was investigated. Neighbour-joining, maximum parsimony, maximum likelihood and Bayesian Inference methods were used for phylogenetic reconstruction. The topology sustained by high bootstrap was: (((Hexabothriidae (Mazocraeidae (Discocotylidae (Diplozoidae (Diclidophoridae (Plectanocotylidae (Heteromicrocotylidae (Microcotylidae (Heteraxinidae), (Thoracocotylidae, Gotocotylidae (Gastrocoylidae (Allodiscocotylidae: Protomicrocotylidae))). In addition, we have also developed DNA barcodes (COI sequences) for six species and the barcodes clearly discriminated all the species. The polytomy within Protomicrocotylidae family is resolved in this study for the first time and it appears that within this family, Bilaterocotyloides species are basal compared to Neomicrocotyle and Lethacotyle species while the latter is the more derived.

scholarly journals A new species of Litarachna Walter, 1925 (Acari: Hydrachnidia: Pontarachnidae) from Corozal Bay (Belize), described based upon morphology and DNA barcodes

Acarologia ◽  
2021 ◽  
Vol 61 (3) ◽  
pp. 602-613
Author(s):  
Lucia Montes-Ortiz ◽  
Tom Goldschmidt ◽  
Lourdes Vásquez-Yeomans ◽  
Manuel Elías-Gutiérrez
Keyword(s):  
Dna Barcode ◽  
Morphological Description ◽  
Estuarine System ◽  
Dna Barcodes ◽  
Sem Images ◽  
The Family ◽  
Coi Sequences ◽  
First Time ◽  
A New Species ◽  
Electronic Microscope

A new planktonic species of the marine water mites of the family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and is augmented by an analysis of DNA cytochrome c oxidase I (COI) sequences, the DNA barcode, used for the first time for a species description in this group.

New records and their associated DNA barcodes of the butterfly family Hesperiidae in Tibet, China

Zootaxa ◽  
2019 ◽  
Vol 4674 (4) ◽  
pp. 426-438
Author(s):  
CHENGLONG CAO ◽  
SIYAO HUANG ◽  
YONGQIANG XU ◽  
HAOMIN WU ◽  
TIANPENG CHEN ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Morphological Study ◽  
New Records ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Species Cluster ◽  
The Family ◽  
Interspecific Genetic Distance ◽  
Coi Sequences ◽  
First Time

The specimens of the family Hesperiidae collected from Tibet during 2016–2018 are identified using morphology. COI sequences of 76 individuals are newly obtained. The result of our morphological study is congruent with COI gene analyses. Maximum likehood (ML) and Bayesina inferences (BI) analyses reveal that individuals identified morphologically as the same species cluster cohesively. The minimum interspecific genetic distance is 1.7% between Halpe aucma and H. filda, and the genetic distance between conspecific individuals ranged from 0 to 0.2% for the genus Halpe. A total of 51 species are recognized, and six of them, Celaenorrhinus consanguineus Leech, 1891, Barca bicolor (Oberthür, 1896), Aeromachus propinquus Alphéraky, 1897, Pedesta bivitta (Oberthür, 1886), Baoris penicillata chapmani Evans, 1937, and Ochlodes brahma Moore, 1878, are reported from Tibet for the first time, and the last species is new to China. 

scholarly journals Internal phylogeny of the Chilopoda (Myriapoda, Arthropoda) using complete 18S rDNA and partial 28S rDNA sequences

1999 ◽  
Vol 354 (1380) ◽  
pp. 215-222 ◽  
Author(s):  
Gonzalo Giribet ◽  
Salvador Carranza ◽  
Marta Riutort ◽  
Jaume Baguñà ◽  
Carles Ribera
Keyword(s):  
18S Rdna ◽  
Gene Sequence ◽  
Sequence Data ◽  
Phylogenetic Reconstruction ◽  
Strong Support ◽  
28S Rdna ◽  
Rdna Sequences ◽  
Mode Of Life ◽  
Branch Support ◽  
28S Rdna Sequences

The internal phylogeny of the ‘myriapod’ class Chilopoda is evaluated for 12 species belonging to the five extant centipede orders, using 18S rDNA complete gene sequence and 28S rDNA partial gene sequence data. Equally and differentially weighted parsimony, neighbour–joining and maximum–likelihood were used for phylogenetic reconstruction, and bootstrapping and branch support analyses were performed to evaluate tree topology stability. The results show that the Chilopoda constitute a monophyletic group that is divided into two lines, Notostigmophora (= Scutigeromorpha) and Pleurostigmophora, as found in previous morphological analyses. The Notostigmophora are markedly modified for their epigenic mode of life. The first offshoot of the Pleurostigmophora are the Lithobiomorpha, followed by the Craterostigmomorpha and by the Epimorpha s. str. (= Scolopendromorpha + Geophilomorpha), although strong support for the monophyly of the Epimorpha s. lat. (= Craterostigmomorpha + Epimorpha s. str.) is only found in the differentially weighted parsimony analysis.

A new species of the genus Sarcinodes Guenée, [1858] (Lepidoptera, Geometridae) from China 

Zootaxa ◽  
2020 ◽  
Vol 4779 (4) ◽  
pp. 553-562
Author(s):  
TIANTIAN YU ◽  
MIN WANG
Keyword(s):  
New Species ◽  
External Morphology ◽  
Female Genitalia ◽  
Dna Barcodes ◽  
Male And Female ◽  
Coi Sequences ◽  
First Time ◽  
A New Species

The genus Sarcinodes Guenée, [1858]of China is reviewed and totally 9 species are recognized with the description of Sarcinodes hainana sp. nov. from Hainan, China. Sarcinodes lilacina Moore, 1888 is reported for the first time from Yunnan, China. The external morphology of adults, particularly the male and female genitalia, are illustrated. COI sequences were obtained as DNA barcodes for identification of the new species. A key to the Chinese Sarcinodes species is provided. 

Involvement of sphaeractinomyxon in the life cycle of mugiliform-infecting Myxobolus (Cnidaria, Myxosporea) reveals high functionality of actinospore morphotype in promoting transmission

Parasitology ◽  
2020 ◽  
Vol 147 (12) ◽  
pp. 1320-1329
Author(s):  
Sónia Rocha ◽  
Luís F. Rangel ◽  
Graça Casal ◽  
Carlos Azevedo ◽  
Pedro Rodrigues ◽  
...  
Keyword(s):  
Life Cycle ◽  
Grey Mullet ◽  
Related Factors ◽  
Rdna Sequences ◽  
The Family ◽  
Collective Group ◽  
Marine Oligochaetes ◽  
Host Identification ◽  
Host Parasite ◽  
First Time

AbstractFour new actinospore types belonging to the sphaeractinomyxon collective group (Cnidaria, Myxosporea) are described from the coelomic cavity of a marine Baltidrilus sp. (Oligochaeta, Naididae) inhabiting a northern Portuguese estuary. Host identification supports the usage of marine oligochaetes, namely of the family Naididae Ehrenberg, 1828, as definitive hosts for myxosporeans inhabiting estuarine/marine environments. The absence of mixed infections in the host specimens analysed is suggested to reflect the influence of host-, parasite- and environmental-related factors regulating myxosporean–annelid interactions. Molecular analyses matched the SSU rDNA sequences of three of the four new types with those of mugiliform-infecting Myxobolus spp., namely Myxobolus mugiliensis and a Myxobolus sp. from flathead grey mullet Mugil cephalus, and Myxobolus labrosus from thicklip grey mullet Chelon labrosus. These results directly link, for the first time, the sphaeractinomyxon collective group to a myxospore counterpart, further confirming their previously hypothesized specific involvement in the life cycle of myxobolids that infect mullets. Acknowledging this life cycle relationship, the functionality of the sphaeractinomyxon morphotype is suggested to have been decisive for the evolutionary hyperdiversification of the genus Myxobolus in mullets. Unlike other actinospore morphotypes, sphaeractinomyxon lack valvular processes, which implies a limited capability for buoyancy. Considering the benthic-feeding nature of mullets, this feature is most likely crucial in promoting successful transmission to the vertebrate host.

Description of the snail-eating flatworm in marine aquaria, Pericelis tectivorum sp. nov. (Polycladida, Platyhelminthes)

Zootaxa ◽  
2019 ◽  
Vol 4565 (3) ◽  
pp. 383 ◽  
Author(s):  
ISABEL L. DITTMANN ◽  
WOLFGANG DIBIASI ◽  
CAROLINA NOREÑA ◽  
BERNHARD EGGER
Keyword(s):  
Ejaculatory Duct ◽  
Phylogenetic Reconstruction ◽  
Marine Snail ◽  
Rdna Sequences ◽  
Internal Morphology ◽  
Long Line ◽  
Differential Characters ◽  
28S Rdna Sequences ◽  
A New Species ◽  
Female Genital

We describe a new marine snail-eating flatworm, Pericelis tectivorum sp. nov., found in coral-bearing marine aquaria. Pericelis tectivorum sp. nov. is characterised by several differential characters of the external and internal morphology like 1) a long line of frontal eyes extending anteriorly; 2) the length of the penis papilla; 3) the spherical seminal vesicle; 4) the lack of the enlargements of the ejaculatory duct; 5) the uterine vesicles, which start posterior of the female genital at the level of the sucker and 6) the distinct sucker. The combination of these characters in one species is unique and therefore the studied specimens are recognised by us as a new species. We additionally present a phylogenetic reconstruction using partial 28S rDNA sequences including three congeners. Our analysis demonstrates that P. tectivorum sp. nov. differs also genetically from other Pericelis species included in this analysis. 

Neoalbatrellus odorus sp. nov. (Albatrellaceae, Russulales) from Southwest China

Phytotaxa ◽  
2017 ◽  
Vol 309 (3) ◽  
pp. 217 ◽  
Author(s):  
YUAN-YUAN CHEN ◽  
MIN WANG ◽  
BO ZHANG ◽  
BAO-KAI CUI
Keyword(s):  
New Species ◽  
Bayesian Inference ◽  
Maximum Likelihood ◽  
Southwest China ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Pore Surface ◽  
Rdna Sequences ◽  
Dark Violet ◽  
28S Rdna Sequences

We describe herein the Neoalbatrellus odorus sp. nov. accounting on its peculiar morphological features and molecular data. It is characterized by fleshy basidiomata with a deep violet to dark violet pileal surface, which turns blackish blue and glossy after drying. It also has a white pore surface, light violet to bluish violet stipe, simple septate generative hyphae, and thick-walled, non-amyloid basidiospores. The phylogenetic analyses, based on ITS and 28S rDNA sequences of Neoalbatrellus and its related genera, were performed using Maximum Parsimony, Maximum Likelihood and Bayesian Inference, confirming the affiliation of the new species to Neoalbatrellus.

Redescription of Acanthogyrus (Acanthosentis) maroccanus (Dollfus, 1951) (Acanthocephala: Quadrigyridae), a parasite of the Algerian barb Luciobarbus callensis (Valenciennes) (Cyprinidae) in Algeria, and first molecular data

2019 ◽  
Vol 94 ◽  
Author(s):  
A. Menasria ◽  
D. Barčák ◽  
N. Kaouachi ◽  
M. Bensouilah ◽  
T. Scholz ◽  
...  
Keyword(s):  
Bayesian Inference ◽  
Maximum Likelihood ◽  
Molecular Data ◽  
Morphological Features ◽  
Phylogenetic Position ◽  
Reproductive Structures ◽  
Rdna Sequences ◽  
Northwest Africa ◽  
28S Rdna Sequences ◽  
Cyprinid Fishes

Abstract Acanthogyrus (Acanthosentis) maroccanus (Dollfus, 1951), an insufficiently described quadrigyrid acanthocephalan of cyprinid fishes from Northwest Africa, is redescribed based on recently collected specimens from the Algerian barb Luciobarbus callensis (Valenciennes) in Algeria. Newly observed morphological features for A. (A.) maroccanus include the arrangement of proboscis hooks (not in regular circles), the male reproductive structures extending into the copulatory bursa and the presence of a para-receptacle structure and vaginal sleeve. The mechanism of copulation of this acanthocephalan is described based on several copulating pairs. The phylogenetic position of A. (A.) maroccanus within Eoacanthocephala was assessed based on partial 28S rDNA sequences. Maximum likelihood and Bayesian inference analyses placed A. (A.) maroccanus in a clade with Palliolisentis (Demidueterospinus) ophiocephalus (Thapar, 1931), both species included in the Quadrigyridae, the only family within the Gyracanthocephala.

scholarly journals Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia

2021 ◽  
Vol 15 (2) ◽  
pp. 149-157
Author(s):  
Viktor V. Bolshakov ◽  
Alexander A. Prokin
Keyword(s):  
Bayesian Inference ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Dna Barcoding ◽  
Molecular Genetic ◽  
Species Level ◽  
Coi Gene ◽  
High Similarity ◽  
Coi Sequences ◽  
First Time

Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried out for this species for the first time. The phylogenetic tree estimated by Bayesian inference showed a high similarity of the studied species with Ch. acutiventris Wülker, Ryser et Scholl, 1983 from the Chironomus obtusidens-group. The estimated genetic distance K2P between Ch. sokolovae and Ch. acutiventris is much lower (0.38%) than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. Our results show that the accepted cytogenetic criteria of species level in the genus Chironomus are more in accordance with morphological ones of the same level, than with molecular-genetic criteria accepted for species COI genetic distance.

scholarly journals New records of water mites from the Balkans revealed by DNA barcoding (Acari, Hydrachnidia)

2021 ◽  
Vol 49 ◽  
pp. 20-34
Author(s):  
Vladimir Pešić ◽  
Milica Jovanović ◽  
Ana Manović ◽  
Ioannis Karaouzas ◽  
Harry Smit
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
Water Mites ◽  
Water Mite ◽  
The Balkans ◽  
Coi Sequences ◽  
First Time

New records of water mites from Serbia, North Macedonia and Greece revealed by DNA barcoding are presented. DNA barcodes were recovered from 63 water mite specimens morphologically assigned to 32 species. One species Lebertia algeriensis Lundblad, 1942, is reported for the first time for the Balkans; four species, i.e. Lebertia fimbriata Thor, 1899, L. rivulorum K. Viets, 1933, Atractides stankovici Pešić & Gerecke, 2010, and Wettina lacustris Pešić & Smit, 2018 are new for North Macedonia; Monatractides madritensis (K. Viets, 1930) and Mideopsis roztoczensis Biesiadka & Kowalik, 1979 are new for Serbia; and Hygrobates mediterraneus Pešić, 2020 is new for Greece. Comparing sequences of our study with the available COI sequences we recognized five possible cases of cryptic diversity involving species clusters with more than 5% divergence indicating that these species need further study.

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