A comparison of three commercial platforms for urinary NGAL in critically ill adults

2016 ◽  
Vol 54 (2) ◽  
Author(s):  
Dinna N. Cruz ◽  
Grazia Maria Virzì ◽  
Alessandra Brocca ◽  
Claudio Ronco ◽  
Davide Giavarina
Keyword(s):  
Rank Correlation ◽  
Kidney Injury ◽  
The Other ◽  
Urine Samples ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Spearman’S Rank Correlation ◽  
Spearman’S Rank Correlation Coefficient ◽  
The Mean ◽  
Critically Ill Adults ◽  
Neutrophil Gelatinase

AbstractEarly biomarkers for acute kidney injury (AKI) diagnosis are needed since an increase in serum creatinine levels is a late marker. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising AKI biomarkers. Prior to routine clinical use, it is necessary to evaluate and validate a high-throughput commercially available method for NGAL detection. The aim of this study was to do an independent validation and comparison of the analytical performance of three different commercially available urine NGAL (uNGAL) assays.Urine samples (n=110) were obtained from various patient groups with and without AKI. All urine samples were processed using Architect NGAL assay, Siemens AdviaOverall, there was good agreement among the three assays: Spearman’s rank correlation coefficient between Architect and Vista was 0.989 (95% confidence interval [CI], 0.983–0.993), between Architect and Advia, 0.962 (95% CI, 0.937–0.977), between Vista and Advia 2400, 0.975 (95% CI, 0.961–0.984). We observed a negative bias of Architect compared with the other assays: comparing Architect to Vista, the mean bias was –55.7 ng/mL (95% CI, –74.3 to –37.0 ng/mL); comparing Architect to Advia 2400, the mean bias was –40.9 ng/mL (95% CI, –56.4 to –25.4 ng/nL). The bias is proportional to the concentration of uNGAL and is more pronounced at higher levels, while irrelevant near the tested cutoff levels of 100 and 190 ng/mL. Comparing Vista and Advia 2400, the mean bias was 10.1 ng/mL (95% CI, 1.5–18.8 ng/mL). Intra-assay imprecision was generally acceptable across all assays; coefficient of variation ranged from 0.8% to 5.3%.All three methods for uNGAL showed acceptable performance for the tested parameters and are comparable with each other at clinically relevant cutoffs. However, Architect yields lower results than the other two methods, with a bias more pronounced at higher uNGAL concentrations, suggesting additional standardization efforts will likely be necessary to better harmonize the uNGAL methods at various clinically relevant cutoffs.

NephroCheck® Quality Test

2020 ◽  
pp. 1-3
Author(s):  
Thummaporn Naorungroj ◽  
Ary Serpa Neto ◽  
Fumitaka Yanase ◽  
Intissar Bittar ◽  
Glenn M. Eastwood ◽  
...  
Keyword(s):  
Risk Score ◽  
Rank Correlation ◽  
Kidney Injury ◽  
Mean Values ◽  
Spearman’S Rank Correlation ◽  
Different Temperatures ◽  
Urine Storage ◽  
The Mean ◽  
The Stability ◽  
Fresh Urine

<b><i>Background:</i></b> The acute kidney injury (AKI) risk score helps detect moderate and severe AKI in the next 12–24 h. However, inappropriate urine collection may impact its results. <b><i>Aim:</i></b> The aim of this study was to evaluate the stability of NephroCheck® after urine storage at different temperatures. <b><i>Methods:</i></b> The urine sample was centrifuged and split into 3 tubes. One was tested as soon as possible by the laboratory. The other 2 samples were frozen at −20 and −80°C, and the NephroCheck® test was performed 8 weeks later. <b><i>Results:</i></b> The mean values of the AKI risk score were 1.19 ± 0.93, 1.15 ± 1.14, and 1.20 ± 1.11 (ng/mL)<sup>2</sup>/1,000 for fresh urine, −20, and −80°C, respectively (<i>p</i> = 0.70). Spearman’s rank correlation for −20 and −80°C versus immediate processing was strong with a rho of 0.82 and 0.98, respectively. <b><i>Conclusion:</i></b> The AKI risk score was relatively stable. Urine could be collected without dry ice or liquid nitrogen and kept for up to 8 weeks with either −20 or −80°C freezing with stable NephroCheck® results.

scholarly journals Urine Neutrophil Gelatinase-Associated Lipocalin Moderately Predicts Acute Kidney Injury in Critically Ill Adults

2009 ◽  
Vol 20 (8) ◽  
pp. 1823-1832 ◽  
Author(s):  
Edward D. Siew ◽  
Lorraine B. Ware ◽  
Tebeb Gebretsadik ◽  
Ayumi Shintani ◽  
Karel G. M. Moons ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Critically Ill ◽  
Kidney Injury ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Critically Ill Adults ◽  
Neutrophil Gelatinase ◽  
Ill Adults

scholarly journals Enhanced detection of atrial tachyarrhythmias with pacing devices by using more accurate atrial sensing

2021 ◽  
Author(s):  
Sami Pakarinen ◽  
Mika Lehto ◽  
Jaap Ruiter ◽  
Willem G. de Voogt
Keyword(s):  
Rank Correlation ◽  
Cardiac Pacing ◽  
Mean Amplitude ◽  
Mode Switching ◽  
Atrial Tachyarrhythmias ◽  
Jude Medical ◽  
Spearman’S Rank Correlation ◽  
Spearman’S Rank Correlation Coefficient ◽  
Group 12 ◽  
The Mean

Abstract Purpose Cardiac pacing devices can detect and monitor atrial tachyarrhythmias (ATA) which increase the risk of thromboembolic complications. The aim of this study was to compare (1) two different atrial leads and (2) standard and optimized settings to detect ATA and reject far-field R-wave signal (FFRW). Methods This was a prospective, randomized multi-center trial comparing St. Jude Medical OptiSense lead (tip-to-ring spacing 1.1 mm) and Tendril lead (tip-to-ring spacing 10.0 mm), having programmed atrial sensitivity at 0.2 mV and post-ventricular atrial blanking at 60 ms. We measured intra-atrial amplitudes of FFRW, intrinsic atrial signals, the amount of FFRW oversensing, and other inappropriate mode switching. Results One hundred and ten patients were enrolled. The mean amplitude of sensed and paced FFRW bipolar signal was 0.13 mV vs. 0.21 mV (p < 0.001) and 0.13 mV vs. 0.26 mV (p < 0.001) with OptiSense and Tendril lead, respectively. The mean amplitude of the atrial bipolar signal was 2.84 mV with OptiSense and 3.48 mV with Tendril lead, p = 0.014. With the optimized settings with OptiSense lead, one patient out of 20 (5%) had FFRW oversensing, none had undersensing of ATAs due to 2:1-blanking of atrial depolarizations, and the concordance of the ATAs by Holter and pacemaker memory was high (Spearman’s rank correlation coefficient = 0.90). In the Tendril group, 12 out of 25 patients (48%) had oversensing and 4 had atrial undersensing (p < 0.001). Conclusions The technique with an atrial lead with short tip-to-ring spacing combined with optimized pacemaker programming resulted in reliable and accurate atrial arrhythmia detection. Trial registration ClinicalTrials.gov number NCT01074749.

scholarly journals Epistaxis limits the performed activities of daily living in proportion to its severity: a cross-sectional survey among patients with hereditary haemorrhagic telangiectasia

2021 ◽  
Vol 4 (4) ◽  
pp. 120-126
Author(s):  
M. Hayama ◽  
Y. Maeda ◽  
T. Tsuda ◽  
H. Akazawa ◽  
A. Nakatani ◽  
...  
Keyword(s):  
Activities Of Daily Living ◽  
Daily Living ◽  
Daily Life ◽  
Rank Correlation ◽  
Hereditary Haemorrhagic Telangiectasia ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Spearman’S Rank Correlation ◽  
Spearman’S Rank Correlation Coefficient ◽  
The Mean

Background: We examined the severity of epistaxis in patients with hereditary haemorrhagic telangiectasia (HHT) and its relationship with the performed activities of daily living. Methodology: This cross-sectional survey included 36 patients with HHT in Japan. An anonymous questionnaire survey was conducted regarding the severity of epistaxis, the measures adopted to prevent epistaxis, and the limitations in the performed activities of daily living. The latter was assessed using a visual analogue scale (VAS). The correlation between epistaxis severity and the VAS score was analysed using Spearman’s rank correlation coefficient. Results: Of the 36 participants surveyed, 94.4% had >1 episode of epistaxis/week. The mean epistaxis severity score (ESS) was 4.3 (range, 0.9–8.4). Limitations in daily life, going out (within a day), meeting with others, eating with others, and going on overnight trips were positively correlated with the ESS. To prevent nosebleeds, 44.4% and 41.7% of the participants used medications and avoided drying their nasal cavities, respectively. Conclusions: Epistaxis impacted the daily life of patients with HHT in proportion to its severity. Nonetheless, less than half of the patients used medications or took precautions. Hence, further educational activities should be considered for medical professionals and patients.

scholarly journals Evaluating Neutrophil Gelatinase-Associated Lipocalin as an Early Marker of Acute Kidney Injury in Critically Ill Patients

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Chettipunyam S Chetan ◽  
Suhas Challa ◽  
Manjunath S Shetty ◽  
Sudarshana Murthy K A ◽  
Kiran K Kelur ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Kidney Injury ◽  
Timely Manner ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Rifle Criteria ◽  
The Mean ◽  
Neutrophil Gelatinase ◽  
Important Marker

Background: Detecting significant renal injury in an accurate and timely manner in acute kidney injury (AKI) patients who are critically ill remains controversial. Serum creatinine (Cr) is an important marker of kidney function in clinical practice, and its limitations are well known. Objectives: This study aimed to evaluate neutrophil gelatinase-associated lipocalin (NGAL) as a marker of the early development of AKI in critically ill AKI patients. Methods: This prospective study was carried out at JSS Hospital, Mysuru, India. The diagnosis and staging of AKI was done according to the RIFLE criteria. Results: A total of 53 critically ill patients were enrolled in this study. During Intensive Care Unit (ICU) stay, 34 (64.2%) patients developed AKI according to RIFLE criteria. Serum NGAL levels assessed on admission were an appropriate predictor of AKI com-pared to serum Cr. Serum NGAL levels also showed a significant elevation among AKI patients than non-AKI cases. The mean levels for AKI patients at 0, 4, and 8 hours were 870.53, 1074.9, and 1090, respectively. Meanwhile, the mean levels for non-AKI patients at 0, 4, and 8 hours were 337, 307, and 292. Conclusions: Measuring serum NGAL on admission is useful in the early diagnosis of AKI com-pared to serum Cr.

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