scholarly journals A Modified Kit Method and a Miniphotometer used for the Rapid Determination of Total Bilirubin in Neonatal Sera

Author(s):  
C H. Konings
1973 ◽  
Vol 19 (12) ◽  
pp. 1366-1369 ◽  
Author(s):  
Gerhard Ertingshausen ◽  
Diane L Fabiny Byrd ◽  
Thomas O Tiffany ◽  
Sandra J Casey

Abstract We report a rapid single-reagent method for determining total bilirubin with the " CentrifiChem Analyzer" (Union Carbide Corp.). The reagent is the stable 1,5-disulfonate of diazosulfanilic acid, and ethylene glycol is used as an accelerator. The reaction requires less than 5 min. Only 25 µl of serum is needed. We present precision data (x = 2.62 mg/dl, SD = .082 mg/dl, CV = 3.13%) and results (r = .998) as compared with those obtained by an automated Jendrassik—Grof method.


1964 ◽  
Vol 10 (2) ◽  
pp. 95-102 ◽  
Author(s):  
G W Stevenson ◽  
S L Jacobs ◽  
R J Henry

Abstract The absorbance at 450 mµ of serum diluted with acidified ethylene glycol is used as a measure of the serum's total bilirubin content in a single-tube, single-extraction method for rapid determination of free and total bilirubin in 0.1 ml. of serum. With the addition of chloroform, free bilirubin is extracted into the chloroform but conjugated bilirubin remains in the ethylene glycol. Free bilirubin is determined from the absorbance at 450 mµ in the chloroform layer. Conjugated bilirubin is calculated from the difference. Absorbance readings at 520 mµ compensate for the absorbance at 450 mµ, due to hemoglobin in the serum. Comparison with the Malloy-Evelyn procedure showed equivalent levels of total but higher levels of conjugated bilirubin.


Author(s):  
T. Y. Tan ◽  
W. K. Tice

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.


2017 ◽  
Vol 45 (2) ◽  
pp. 455-464
Author(s):  
T.T. Xue ◽  
J. Liu ◽  
Y.B. Shen ◽  
G.Q. Liu

2013 ◽  
Vol 40 (11) ◽  
pp. 1725-1729
Author(s):  
Lang ZHOU ◽  
Qiao-Jing LI ◽  
Yong-Sheng LI ◽  
Xiu-Feng GAO

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