Parameter estimation of support vector machine with radial basis function kernel using grid search with leave-p-out cross validation for classification of motion patterns of subviral particles

The classification of subviral particle motion in fluorescence microscopy video sequences is relevant to drug development. This work introduces a method for estimating parameters for support vector machines (SVMs) with radial basis function (RBF) kernels using grid search with leave-pout cross-validation for classification of subviral particle motion patterns. RBF-SVM was trained and tested with a large number of combinations of expert-evaluated training and test data sets for different RBF-SVM parameters using grid search. For each subtest, the mean and standard deviation of the accuracy of the RBF-SVM were calculated. The RBF-SVM parameters are selected according to the optimal accuracy. For the optimal parameters, the accuracy is 89% +- 13% for N = 100. Using the introduced computer intensive machine learning parameter adjustment method, an RBF-SVM has been successfully trained to classify the motion patterns of subviral particles into chaotic, moderate and linear movements.