Determination of β-Lactamase Activities and Antibiotic Susceptibility of Some Bacillus Strains Causing Food Poisoning

2001 ◽  
Vol 18 (1) ◽  
Author(s):  
G. Uraz ◽  
H. Şimşek ◽  
Y. Maraş
Keyword(s):  
Antibiotic Susceptibility ◽  
Food Poisoning ◽  
Bacillus Strains

scholarly journals Incidence of Escherichia coli  - Glucuronidase Positive on Goat Milk

2016 ◽  
Vol 73 (2) ◽  
pp. 359
Author(s):  
Zorica Voşgan ◽  
Anca Domuţa ◽  
Stela Jelea ◽  
Lucia Mihălescu ◽  
Flavia Pop
Keyword(s):  
Escherichia Coli ◽  
Food Poisoning ◽  
Goat Milk ◽  
Lactation Period ◽  
Health Indicator ◽  
Pathogenic Factor ◽  
E Coli ◽  
Higher Temperature ◽  
Hot Weather

Papers on beta- glucuronidase sensitivity and specificity for identifying Escherichia coli in sources of environment, food, water, etc. have been published since 1976. In this study we conducted a review of the incidence of E. coli β- glucuronidase -positive in goat milk, obtained by hand milking throughout the lactation: spring, summer, autumn. The presence of E. coli in milk is considered both as a health indicator and a pathogenic factor capable of causing food poisoning. The determination of the E. coli β-glucuronidase-positive was carried using TBX medium by cultivating colonies typical blue at 440C. The absence of E. coli in milk yielded during the spring, when the animal milking is done three times a day, was found in the performed analyses; the same was observed during fall, when the milk production is lower and the milking is done once a day. The load of E. coli β-glucuronidase-positive was averaging 66.67 CFU/ml of goat milk, during the middle lactation period (July-August), in conditions of higher temperature. During this period, milking is done in the mountain zone, where the transhumance of animals takes place in summer. The presence of the species E. coli was also confirmed by microscopic examination. Attention should be paid to hygiene and milk should be immediately cooled, during hot weather, as E. coli can be a source of food poisoning.  

scholarly journals Coherent fluctuation nephelometry in clinical microbiology

2019 ◽  
Vol 9 (2) ◽  
pp. 385-392
Author(s):  
A. S. Gur’ev ◽  
O. Yu. Shalatova ◽  
E. V. Rusanova ◽  
I. V. Vasilenko ◽  
A. Yu. Volkov
Keyword(s):  
Antibiotic Susceptibility ◽  
Clinical Microbiology ◽  
Urine Analysis ◽  
Growth Curves ◽  
High Sensitivity ◽  
Pure Cultures ◽  
Susceptibility Tests ◽  
Express Methods ◽  
Coherent Fluctuation

In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology.

scholarly journals The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms

1999 ◽  
Vol 37 (6) ◽  
pp. 1771-1776 ◽  
Author(s):  
H. Ceri ◽  
M. E. Olson ◽  
C. Stremick ◽  
R. R. Read ◽  
D. Morck ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Clinical Laboratory ◽  
Rapid Determination ◽  
New Technology ◽  
Growth Curves ◽  
National Committee ◽  
Standard Group ◽  
Antibiotic Susceptibilities ◽  
Significant Difference

Determination of the MIC, based on the activities of antibiotics against planktonic bacteria, is the standard assay for antibiotic susceptibility testing. Adherent bacterial populations (biofilms) present with an innate lack of antibiotic susceptibility not seen in the same bacteria grown as planktonic populations. The Calgary Biofilm Device (CBD) is described as a new technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. The CBD produces 96 equivalent biofilms for the assay of antibiotic susceptibilities by the standard 96-well technology. Biofilm formation was followed by quantitative microbiology and scanning electron microscopy. Susceptibility to a standard group of antibiotics was determined for National Committee for Clinical Laboratory Standards (NCCLS) reference strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, andStaphylococcus aureus ATCC 29213. Growth curves demonstrated that biofilms of a predetermined size could be formed on the CBD at specific time points and, furthermore, that no significant difference (P > 0.1) was seen between biofilms formed on each of the 96 pegs. The antibiotic susceptibilities for planktonic populations obtained by the NCCLS method or from the CBD were similar. Minimal biofilm eradication concentrations, derived by using the CBD, demonstrated that for biofilms of the same organisms, 100 to 1,000 times the concentration of a certain antibiotic were often required for the antibiotic to be effective, while other antibiotics were found to be effective at the MICs. The CBD offers a new technology for the rational selection of antibiotics effective against microbial biofilms and for the screening of new effective antibiotic compounds.

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