scholarly journals Determination of peptidase production potential of Bacillus strains isolated from ropey bread and optimisation of some culture conditions for peptidase production

Author(s):  
Muharrem CERTEL ◽  
Fundagül EREM
2008 ◽  
Vol 41 (7) ◽  
pp. 1436-1449 ◽  
Author(s):  
Christophe Provin ◽  
Kiyoshi Takano ◽  
Yasuyuki Sakai ◽  
Teruo Fujii ◽  
Ryo Shirakashi

1988 ◽  
Vol 15 (3) ◽  
pp. 201-207
Author(s):  
Karel Lavrijsen ◽  
Derk Van Dijck ◽  
Jos Van Houdt ◽  
Ingrid Van Pelt ◽  
Willem Meuldermans ◽  
...  

Isolated hepatocytes from male rats were cultured in primary cell culture in 24-well dishes for 20–24 hours in the presence of the various test compounds. Cytotoxicity at the end of the culture period was evaluated by the determination of LDH-release into the culture medium or by quantification of DNA content as a measure of the cell number in each well. Toxic xenobiotics, such as chlorpromazine, were able to induce LDH-release at up to 5 times the background levels at concentrations of 10-4–10-5 M. For most compounds, the increase in LDH-release was closely related to a decrease in cell number, as measured by the DNA method. Two culture conditions which might influence the cytotoxic response were investigated. Prolongation of the culture time increased the toxicity of some compounds, e.g. sodium dodecylsulphate, whereas for other compounds, e.g. amitriptyline, no change was noted. Addition of serum albumin and α1-acid glycoprotein decreased the toxicity of chlorpromazine to isolated hepatocytes. These results indicate that isolated hepatocytes might represent a useful in vitro toxicity screening system, and that careful standardisation of the culture conditions is necessary.


2019 ◽  
Vol 43 (4) ◽  
pp. 395-404 ◽  
Author(s):  
Belgin COŞGE ŞENKAL ◽  
Tansu USKUTOĞLU ◽  
Cüneyt CESUR ◽  
Volkan ÖZAVCI ◽  
Hülya DOĞAN

Author(s):  
I. L. Uydess ◽  
Wolf Vishniac

A modified negative staining technique (“OTPT”) has been described in the study of the surface topography of bacteria. This technique is useful in the rapid and accurate identification and discrimination of the Gram reaction of unknown bacteria under circumstances unfavorable to the direct application of the Gram stain. Cells of a particular genus exhibit the same basic topographical features independent of cell age, culture conditions or other cellular parameters.Several pure cultures of unclassified bacteria isolated from Antarctic soils were prepared by the OTPT staining method. Although initial attempts employing the Gram stain were often inconclusive, this technique allowed a positive identification of all samples. Two organisms were chosen for more detailed study.


2020 ◽  
Author(s):  
Harrison Charo-Karisa ◽  
Jacob Maithya

Abstract This paper discussed the conservation efforts of fish farmers of two endangered fish species in Lake Victoria namely Oreochromis variabilis and O. esculentus. Highlights focused on the determination of their growth performance under culture conditions, assessing their suitability for aquaculture, recruiting farmers to culture the species and testing the suitability of new dams and ponds for aquaculture. Both species breed easily under culture conditions. Therefore production of the fingerlings and their subsequent stocking in ponds, small water bodies and other larger water masses, including Lake Victoria, was a course of action implemented by the fish farmers to bring about their restoration.


2012 ◽  
Vol 13 (1) ◽  
pp. 47 ◽  
Author(s):  
Mariano Córdoba ◽  
Mónica Balzarini ◽  
Cecilia Bruno ◽  
José Luis Costa

<p>El manejo sitio-específico demanda la identificación de sub-regiones homogéneas, o zonas de manejo (ZM), dentro del espacio productivo. Sin embargo, definir ZM suele ser complejo debido a que la variabilidad espacial del suelo puede depender de varias variables. La zonificación o delimitación de ZM puede realizarse utilizando una variable de suelo a la vez o considerando varias variables simultáneamente. Entre los métodos de análisis multivariado, difundido para la zonificación, se encuentra el análisis de conglomerados fuzzy k-means (KM) y el análisis de componentes principales (PCA). No obstante, como otros métodos multivariados, éstos no han sido desarrollados específicamente para datos georreferenciados. Una nueva versión del PCA, conocido como MULTISPATI-PCA (PCAe), permite contemplar la autocorrelación espacial entre datos de variables regionalizadas. El objetivo de este estudio fue proponer una nueva estrategia de análisis para la identificación de ZM, combinando la aplicación KM y PCAe sobre datos de múltiples variables de suelo. La capacidad del método propuesto se evaluó en base a la comparación de los rendimientos promedios alcanzados en cada zona delimitada, tanto para la combinación de KM con PCA, la aplicación tradicional de KM sobre las variables originales y la nueva propuesta KM-PCAe. Los resultados mostraron que KM-PCAe fue el único método que permitió distinguir zonas estadísticamente diferentes en cuanto al potencial productivo. Se concluye que la combinación propuesta constituye una herramienta importante para el mapeo de la variabilidad espacial y la identificación de ZM a partir de datos georreferenciados. </p><p> </p><p><strong>Identification of site-specific management zones from combination of soil variables</strong></p>Site-specific management demands the identification of homogeneous subfield regions within the field or management zones (MZ). However, due to the spatial variability of soil variables, determination of MZ from several variables, is often complex. Although the zonification or delimitation of MZ may be univariate, it is more appropriate to consider all variables simultaneously. Fuzzy k-means clustering (KM) and principal component analysis (PCA) are multivariate analyses that have been used for zonification. Nevertheless, PCA and KM have not been explicitly developed for georeferenced data. Novel versions of PCA, known as MULTISPATI-PCA (PCAe), incorporate spatial autocorrelation among data of neighbor sites of regionalized variables. The objective of this study was to propose a new analytical tool to identify homogeneous zones from the combination of KM and PCAe on multiple soil variable data. The performance of proposed method was assessed through comparison of the average yields obtained in each zone delimited by combination of KM with PCA, as well as KM on the original variables and the new proposed method KM-PCAe. The results showed that KM-PCAe was the only method able to identify zones statistically different in terms of production potential. PCAe and its combination with KM are useful tools to map spatial variability and to identify MZ within fields from georeferenced data.


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