scholarly journals Plant endophytic fungi as a source of paclitaxel

2015 ◽  
Vol 60 (4) ◽  
pp. 22-33 ◽  
Author(s):  
Alicja Michalczyk ◽  
Anna Cieniecka-Rosłonkiewicz ◽  
Małgorzata Cholewińska
Keyword(s):  
Antitumor Activity ◽  
Endophytic Fungi ◽  
Spectroscopic Analysis ◽  
Tumor Development ◽  
Corylus Avellana ◽  
Ocimum Basilicum ◽  
Fungal Culture ◽  
Significant Extent ◽  
Chromatography Method ◽  
Layer Chromatography

SummaryEndophytic fungi were isolated from different parts of the plants Corylus avellana (Corylaceae) and Ocimum basilicum (Lamiaceae) and then identified to the genus level based on the morphology of the fungal culture and the characteristics of the spores. The fungicidal and antitumor activity of isolates and extracts obtained from them was determined. We found that a few isolates from Corylus avellana and Ocimum basilicum produced metabolites that inhibited the growth of Oomycetes fungi to a highly significant extent. In the potato disc bioassay only an extract from the isolate C-9 showed an inhibitory action in tumor development. Paclitaxel presence in extract from the isolate C-9 was confirmed using the thin layer chromatography method (TLC) and UV-VIS spectroscopic analysis. The above results indicated that the tested samples revealed an antitumor activity. The detection of paclitaxel in the isolate C-9 suggests that the antitumor activity resulted probably from the presence of this taxane in the tested material.

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

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