scholarly journals Wild boar offal as a probable source of Aujeszky’s disease virus for hunting dogs in Poland

2016 ◽  
Vol 60 (3) ◽  
pp. 233-238
Author(s):  
Anna Szczotka-Bochniarz ◽  
Andrzej Lipowski ◽  
Anna Kycko ◽  
Bartosz Sell ◽  
Michał Ziółkowski ◽  
...  
Keyword(s):  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Neurological Disorders ◽  
Virus Isolation ◽  
Tissue Samples ◽  
Hunting Dogs ◽  
First Case ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease

AbstractIntroduction: Aujeszky’s disease (AD), most often related to infection of domestic and feral swine, may also concern other mammals, including dogs. The disease in carnivores, related to consumption of raw meat or offal contaminated with AD virus, is manifested by severe neurological disorders and inevitably leads to animal’s death.Material and Methods: Karelian bear dog was euthanised due to nervous symptoms that started two days after participation in wild boar hunting. After exclusion of rabies the dog’s carcass was subjected to standard necropsy. Tissue samples were collected for histological examination. Samples of the brain were tested for ADV by real-time PCR and virus isolation. Samples of the liver were collected for toxicological examination.Results: The presence of ADV was confirmed by real-time PCR and virus isolation. Toxicological examination revealed anticoagulant poisoning. This is the first case of Aujeszky’s disease (AD) in a hunting dog in Poland after exposure to ADV from offal of wild boar.Conclusion: This infection should be taken into consideration in differential diagnosis of syndromes of neurological disorders in dogs. Since AD is found in both domestic pigs and wild boar in Poland, special care must be taken to prevent spread of infection to other species.

scholarly journals Aujeszky’s disease in hunting dogs after the ingestion of wild boar raw meat in Sicily (Italy): clinical, diagnostic and phylogenetic features

2022 ◽  
Vol 18 (1) ◽  
Author(s):  
Flavia Pruiti Ciarello ◽  
Ana Moreno ◽  
Nicola Miragliotta ◽  
Aliberti Antonino ◽  
Michele Fiasconaro ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Wild Boar ◽  
Severe Disease ◽  
Fatal Outcome ◽  
Raw Meat ◽  
Hunting Dogs ◽  
Clinical Diagnostic ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Clade 1

Abstract Background Aujeszky's disease is caused by Suid Herpes Virus-1 and species belonging to the genus Sus scrofa are the main reservoir hosts. This virus, however, is capable of infecting and causing severe disease, with an almost constant fatal outcome in other species, both domestic and wild (carnivores, monogastric herbivores and ruminants). Moreover, the possibility of transmission to humans has been demonstrated. This study reports and describes the clinical, diagnostic, pathological and phylogenetic aspects of two cases of Aujeszky's disease in two hunting dogs following the ingestion of infected wild boar raw meat. These cases are contextualized in the province of Messina (Sicily), where a high prevalence of Aujeszky's disease has been recorded (average of 12,20% in the period 2010–2019) in farmed pig, and with evidence of spread to other species. A severe outbreak in cattle has recently been reported in these areas. Nevertheless, cases of Aujeszky's disease in dogs are rarely reported and this study represents the first well-documented report in this species in Sicily. Case presentation After a wild boar hunt, two dogs showed neurological symptoms and intense itching unresponsive to therapy. Diagnosis of Aujeszky's disease was made based on clinical suspicion, anamnestic information and confirmed by the isolation of the virus from the brain of both dogs. In addition, molecular typing, sequencing and phylogenetic analysis of the Real-Time PCR products were performed. The sequences studied were placed in the Italian Clade 1 along with the sequences obtained from wild boars and hunting dogs from Italy and France. Conclusions The finding of this disease in non-natural hosts in Sicilian multi-host epidemiological contexts suggests that the risk of inter-species transmission is concrete and that attention should be paid to developing disease control programs in these territories. The data obtained from genome sequencing of the two SuHV-1 isolates contribute to the enrichment of the GenBank with unknown sequences and the phylogenetic analysis implementation.

scholarly journals Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy

2020 ◽  
Vol 14 (12) ◽  
pp. e0008982
Author(s):  
Giovanni Cilia ◽  
Fabrizio Bertelloni ◽  
Ivana Piredda ◽  
Maria Nicoletta Ponti ◽  
Barbara Turchi ◽  
...  
Keyword(s):  
Wild Boar ◽  
Real Time ◽  
Reproductive System ◽  
Real Time Pcr ◽  
Sus Scrofa ◽  
Positive Real ◽  
Pathogenic Leptospira ◽  
Tissue Samples ◽  
Italian Regions ◽  
Venereal Transmission

Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira (lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira.

scholarly journals Real-time PCR strategy for parasite quantification in blood and tissue samples of experimental Trypanosoma cruzi infection

Acta Tropica ◽  
2012 ◽  
Vol 123 (3) ◽  
pp. 170-177 ◽  
Author(s):  
Sérgio Caldas ◽  
Ivo Santana Caldas ◽  
Lívia de Figueiredo Diniz ◽  
Wanderson Geraldo de Lima ◽  
Riva de Paula Oliveira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Real Time ◽  
Real Time Pcr ◽  
Tissue Samples ◽  
Cruzi Infection

Occurrence of Aujeszky’s disease in wild boar (Sus scrofa) in Slovakia

Biologia ◽  
2021 ◽  
Author(s):  
Gabriela Čonková-Skybová ◽  
Silvia Zemanová ◽  
Katarína Bárdová ◽  
Peter Reichel ◽  
Róbert Link ◽  
...  
Keyword(s):  
Wild Boar ◽  
Sus Scrofa ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease

AUJESZKY'S DISEASE IN HUNTED WILD BOAR (SUS SCROFA) IN THE IBERIAN PENINSULA

2021 ◽  
Vol 57 (3) ◽  
Author(s):  
Alexandra Müller ◽  
Natacha Melo ◽  
David González-Barrio ◽  
Madalena Vieira Pinto ◽  
Francisco Ruiz-Fons
Keyword(s):  
Iberian Peninsula ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease

scholarly journals Ability of Real-time PCR in diagnose Differentiation Various Forms of Cutaneous Leishmaniasis: A Comparative Study with Histopathology

2019 ◽  
Author(s):  
Maryam Fekri Soofi Abadi ◽  
Meisam Fekri ◽  
alireza moradabadi ◽  
Reza Vahidi ◽  
Simin Shamsi Meymandi ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Real Time ◽  
Real Time Pcr ◽  
Relative Number ◽  
Parasite Load ◽  
Detection Methods ◽  
Tissue Samples ◽  
Microscopic Evaluation ◽  
Histopathological Evaluation ◽  
Histopathological Studies

Abstract objective: Histopathological studies suggest that parasite load is different between acute and chronic forms of cutaneous leishmaniasis (CL). However, highly sensitive detection methods are still needed to distinguish different forms of leishmaniasis. In the present study, we developed a quantitative real-time polymerase chain reaction (PCR) to detect and quantify leishmania tropica parasites in paraffin-embedded tissue samples. Results: The ability of real-time PCR for leishmania detection was higher than histopathological evaluation. The parasite loads were quantified by qPCR assay and microscopic evaluation were highly correlated ( r =0.598; P <0.001). Among patients, the parasite load was inversely correlated with disease duration (acute CL lesions had very higher parasite loads than chronic CL lesions), but there was no difference in parasite load according to the patients’ age and sex as well as location of the lesions. In contrast to Ridley scoring system (P<0.001), there were no statistically significant differences in the relative number of parasites among the lupoid and non-lupoid forms of chronic lesions in real-time PCR (P=0.549), which indicates the superiority of histopathological evaluation in CL forms differentiation.

scholarly journals Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

2016 ◽  
Vol 60 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Aliasghar Bahari ◽  
Masoud Sabouri Ghannad ◽  
Omid Dezfoulian ◽  
Fereydon Rezazadeh ◽  
Ali Sadeghi-Nasab
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mediastinal Lymph Node ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Tissue Samples ◽  
Blood Samples ◽  
Proviral Dna ◽  
Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

scholarly journals Report of two unlinked cases of infant botulism in the UK in October 2007

2009 ◽  
Vol 58 (12) ◽  
pp. 1601-1606 ◽  
Author(s):  
Kathie A. Grant ◽  
Ijeoma Nwarfor ◽  
Obioma Mpamugo ◽  
Vina Mithani ◽  
Paula Lister ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clostridium Botulinum ◽  
Diagnostic Procedure ◽  
Causative Organism ◽  
Mouse Bioassay ◽  
Young Infants ◽  
First Case ◽  
Infant Botulism ◽  
The Uk

Infant botulism is a rare disease in the UK, with the first case being recognized in 1978 and only five subsequent cases being reported before 2007. This study reports two unlinked cases of infant botulism, caused by two distinct strains of Clostridium botulinum (toxin types A and B, respectively), that occurred within a single month in the south-east of England in October 2007. The use of real-time PCR to detect C. botulinum neurotoxin genes in clinical specimens to improve the diagnostic procedure and to follow carriage of the causative organism in the infant gut is described. The laboratory investigation of these two cases demonstrated that a combination of the mouse bioassay, real-time PCR assays and conventional microbiological culture can provide rapid confirmation of a clinical diagnosis and affect patient management. Both infants (aged 4 and 8 months) were previously healthy prior to the onset of symptoms, and in both cases, a diagnosis of infant botulism was delayed for at least 10 days after initial admission to hospital. Once diagnosed, one of the infants was the first in the UK to be treated with human-derived botulism immunoglobulin. Real-time PCR was used to demonstrate that C. botulinum was excreted in the infants' faeces for up to 68 and 81 days, respectively. Despite the infrequency of infant botulism in the UK, clinicians should be aware of this rare but serious condition and should seek microbiological advice when presented with young infants with compatible symptomologies.

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