Trophic and symbiotic links between obligate-glacier water bears (Tardigrada) and cryoconite microorganisms

Insights into biodiversity and trophic webs are important for understanding ecosystem functions. Although the surfaces of glaciers are one of the most productive and biologically diverse parts of the cryosphere, the links between top consumers, their diet and microbial communities are poorly understood. In this study, for the first time we investigated the relationships between bacteria, fungi and other microeukaryotes as they relate to tardigrades, microscopic metazoans that are top consumers in cryoconite, a biologically rich and productive biogenic sediment found on glacier surfaces. Using metabarcoding (16S rDNA for bacteria, ITS1 for fungi, and 18S rDNA for other microeukaryotes), we analyzed the microbial community structures of cryoconite and compared them with the community found in both fully fed and starved tardigrades. The community structure of each microbial group (bacteria, fungi, microeukaryotes) were similar within each host group (cryoconite, fully fed tardigrades and starved tardigrades), and differed significantly between groups, as indicated by redundancy analyses. The relative number of operational taxonomic units (ZOTUs, OTUs) and the Shannon index differed significantly between cryoconite and tardigrades. Species indicator analysis highlighted a group of microbial taxa typical of both fully fed and starved tardigrades (potential commensals), like the bacteria of the genera Staphylococcus and Stenotrophomonas, as well as a group of taxa typical of both cryoconite and fully fed tardigrades (likely part of the tardigrade diet; bacteria Flavobacterium sp., fungi Preussia sp., algae Trebouxiophyceae sp.). Tardigrades are consumers of bacteria, fungi and other microeukaryotes in cryoconite and, being hosts for diverse microbes, their presence can enrich the microbiome of glaciers.

Archerfish number discrimination

Debates have arisen as to whether non-human animals actually can learn abstract non-symbolic numerousness or whether they always rely on some continuous physical aspect of the stimuli, covarying with number. Here we investigated archerfish (Toxotes jaculatrix) non-symbolic numerical discrimination with accurate control for co-varying continuous physical stimulus attributes. Archerfish were trained to select one of two groups of black dots (Exp. 1: 3 vs. 6 elements; Exp. 2: 2 vs. 3 elements); these were controlled for several combinations of physical variables (elements’ size, overall area, overall perimeter, density and sparsity), ensuring that only numerical information was available. Generalization tests with novel numerical comparisons (2 vs. 3, 5 vs. 8 and 6 vs. 9 in Exp. 1; 3 vs. 4, 3 vs. 6 in Exp. 2) revealed choice for the largest or smallest numerical group according to the relative number that was rewarded at training. None of the continuous physical variables, including spatial frequency, were affecting archerfish performance. Results provide evidence that archerfish spontaneously use abstract relative numerical information for both small and large numbers when only numerical cues are available.

The Dynamics of Privately Held Business Assets in the United States

Although privately held businesses are central to the American economy, little is known about how their assets are distributed among the population. In this study, we describe the household distribution of private business assets in the United States and examine how it has changed over time. Using data from the 1989-2019 Survey of Consumer Finances, we show that the relative number of business owners has remained stagnant at low levels and that assets in privately held firms have become increasingly concentrated among the wealthiest owners over time. At the most recent wave of data collection, the top 1% of households controlled nearly 80% of private business assets, up from about 70% in the late 1980s. We attempt to explain this trend by evaluating how technological change, the financialization of banking, and rising market power have influenced the distribution of private business assets in recent decades. Our findings suggest that all three factors contributed to increasing asset concentration in this sector.

The economic impact of audit failures

This paper examines the economic consequences associated with an audit failure in the field of statutory auditing services, by analyzing changes in the audit firm’s market share around the time of the investigation process undertaken by the Spanish Public Oversight Board. We explore the variations in audit market share by applying the difference in differences method to a treatment group of 70 sanctioned audit firms and a matched control group of 70 non-sanctioned audit firms. The period of analysis covers the years from 1999 to 2015. Our results show that the sanctioned audit firms suffered a significant decrease in their relative number of clients. Moreover, this measure of market share decreased not only after the publication of the sanction disclosure (which may be attributed to reputational losses) but also after the initiation of the investigation (which may be attributed to the firm’s reluctance to audit risky clients). Findings are similar for both small and large firms when the market share is measured in terms of clients, whereas the evidence is weak concerning variations in their turnover-based market share. Our conclusions could be of interest for audit firms and also for audit regulators when designing disciplinary systems. Este trabajo examina las consecuencias económicas asociadas a un fallo de auditoría en el ámbito de los servicios de auditoría legal, analizando los cambios en la cuota de mercado de la firma de auditoría en torno al momento del proceso de investigación emprendido por el Consejo de Supervisión Pública español. Exploramos las variaciones en la cuota de mercado de la auditoría aplicando el método de diferencia en diferencias a un grupo de tratamiento de 70 firmas de auditoría sancionadas y a un grupo de control emparejado de 70 firmas de auditoría no sancionadas. El periodo de análisis abarca los años comprendidos entre 1999 y 2015. Nuestros resultados muestran que las firmas de auditoría sancionadas sufrieron un descenso significativo en su número relativo de clientes. Además, esta medida de la cuota de mercado disminuyó no sólo después de la publicación de la comunicación de la sanción (lo que puede atribuirse a las pérdidas de reputación), sino también después del inicio de la investigación (lo que puede atribuirse a la reticencia de la empresa a auditar clientes de riesgo). Los resultados son similares tanto para las pequeñas como para las grandes empresas cuando la cuota de mercado se mide en términos de clientes, mientras que la evidencia es débil en lo que respecta a las variaciones de su cuota de mercado basada en el volumen de negocio. Nuestras conclusiones podrían ser de interés para las firmas de auditoría y también para los reguladores de la auditoría a la hora de diseñar sistemas disciplinarios.

Features of bioaccumulation and toxic effects of copper (II) oxide nanoparticles under the oral route of intake into the body

Introduction. Active use in various spheres of economic activity and the large-scale nature of production determine the relevance of studying the effects of copper (II) oxide nanoparticles (CuO NPs) on the body during the oral route of intake. Material and methods. Particle size was determined by scanning electron microscopy and dynamic laser light scattering; specific surface area - Brunauer, Emmett and Teller; total pore volume - Barrett, Joyner and Khalenda. Acute oral toxicity of CuO NPs was studied in Wistar rats in accordance with GOST 32644-2014, multiple oral toxicity was studied by the Lim method. After repeated exposure, the biochemical and hematological parameters of the blood, the concentration of copper in the organs, and pathomorphological changes in the tissues of the organs were determined. Results. The size of CuO NPs in the composition of the native powder was 45.86 nm, in the aqueous suspension - 307.40 nm, the specific surface area was 17.70 m2/g, and the total pore volume was 0.056 cm3/g. According to the results of a single oral exposure, the LD50 value was > 2000 mg / kg body weight, which corresponds to 3 (GOST 12.1.007-76) and 4 (GOST 32644-2014) hazard classes. With repeated oral exposure, an increase in the levels of activity of ALT, AST, ALP, LDH, amylase, AOA and MDA was noted; the relative number of segmented neutrophils is increased, the number of leukocytes is increased, the relative number of lymphocytes is reduced. The concentration of copper under the action of NPs increases in the lungs, liver, stomach, intestines, kidneys, brain and blood. Pathomorphological changes in the tissues of the liver, kidneys, stomach, small and large intestines and lungs were established. Conclusion. The results obtained prove the presence of toxic properties of CuO NPs and can be used in the development of preventive measures for workers and consumers in contact with products containing CuO NPs.

The role of Herpesviridae infection in the progression of comorbid somatic pathology

Background. The objective was to study the peculiarities of cellular and humoral immunity in patients with chronic obstructive pulmonary disease (COPD) group B and C, GOLD 2–3, who have signs of metabolic syndrome (MS) and Herpesviridae infection. Materials and methods. Forty-two patients with B and C groups of COPD, GOLD 2–3, associated with MS were examined. Eighteen individuals had COPD combined with MS, and Herpesviridae infection. The average age of patients was 51.3 ± 4.2 years. The comparison group included 24 people with COPD and MS without signs of Herpesviridae infection. All patients underwent determination of herpes simplex virus (HSV) type 1 and cytome­galovirus (CMV) antigens in the blood and saliva, titer of specific immunoglobulin (Ig) G and IgM antibodies to HSV type 1 and CMV, a comprehensive immunological examination with a study of cellular and humoral immunity indexes, cytokine status. Results. The study of the activity of herpes viruses in the blood of patients of the main group did not reveal active replication of HSV type 1 and CMV; in the saliva of 15 individuals (83.3 %), an active replication of HSV type 1 was detected, and in 12 people (66.7%) — of CMV. All patients in the main group had a severe course of Herpesviridae infection with an exacerbation rate of more than 6 times a year. The immunological status of patients of the main group showed immunodeficiency mainly of T-cell immune system and NK-cells, a significant increase in the relative number of T- and B-lymphocytes with early and late markers of activation on the background of autoimmune manifestations and inflammatory changes in peripheral blood. Conclusions. The presence of chronic persistent infection of HSV type 1 and CMV causes a severe course of COPD associated with MS, induces the development of infectious exacerbations of COPD and more significant manifestations of systemic inflammation in atherosclerosis as a morphological substrate of MS.

Penerapan Metode AHP dan OCRA dalam Pengambilan Keputusan Menentukan Santri Berprestasi

The selection of outstanding students is an appreciation given by the boarding school to the students who have the highest scores. However, the election is usually still done subjectively and manually, and there is no support system in creating decisions, which make the selection process long and inaccurate. This study aims to implement a decision support system in determining outstanding students at Mamba'ussholihin Islamic Boarding School predetermined with criteria. The process of selecting uses the method Analytical Hierarchy Process (AHP) for weighting criteria and Operational Competitiveness Rating Analysis (OCRA) for ranking. The OCRA value obtained is in the form of Pi, which is ranked to get the best students. The findings show that the error consistency level is less than 10% and the consistency index value is accepted, so that it can be used as an initial weight with a relative number = 1. After calculating the OCRA method from the initial 75 alternative data, the top 10 alternative data obtained are in the form of the value of Pi will be entitled to an award as an outstanding student, so by applying these two methods it can speed up the results of the decision, it does not take a long time and the results obtained are also precise.

Hemorrhagic colitis syndrome in children: the etiological structure in the infectious diseases clinic

The range of differential diagnostic search in the development of hemorrhagic colitis (hemocolitis) is wide enough and includes infectious and non-infectious factors. Purpose: clinical, laboratory and etiological analysis of bacterial diarrhea occurring with hemorrhagic colitis in the infectious diseases department.Materials and methods: a retrospective study of 141 case histories of those hospitalized in the infectious diseases department of the2 Children's City Clinical Hospital No. 9 named after G.N. Speransky of Moscow in 2019—2021 patients with clinical picture of hemocolitis. Hemocolitis was determined on the basis of macroscopic and microscopic signs (in scatology — mucus, leukocytes, erythrocytes) signs. All patients underwent routine laboratory examinations. The etiology was verified using modern methods of laboratory diagnostics (bacteriological analysis of feces, Latex test, ICA, ELISA, PCR, IHR).Results. There were 137 patients with infectious hemocolitis. Inflammatory bowel disease was diagnosed in 3 children, anus fissure — in 1 child. Young children under 3 years of age prevailed (77%). The etiology of infectious hemocolitis was deciphered in 47 patients (34%). Salmonellosis (36%) and shigellosis (36%) prevailed. Campylobacteriosis, clostridiosis-dificile and klebsiellosis accounted for 11%, 9% and 6% of cases, respectively. Yersiniosis was detected in 1 child at the age of 5 months. The severe form was found in 5.8% of cases, in most cases with shigellosis. Symptoms of intoxication and febrile fever were expressed in all patients, vomiting — in 28.5%, abdominal pain — in 94%, mesenteric adenitis on ultrasound — in 15%, diarrhea with a frequency of more than 5 times a day — in 84%, dehydration — in 64%, intercurrent diseases (ARVI, pneumonia) — in 41.3% of cases. Inflammatory changes in infectious hemocolitis were manifested by an increase in C-reactive protein in 71% (23.91 ± 24.17 mg/l), leukocytosis — in 69% (11.58 ± 3.52 х103 / μl), thrombocytosis — in 26%, an increase in the relative number of stab neutrophils in the general blood test in 78% of cases (10.95 ± 0.4%).Conclusions. Differential diagnostic search in the development of hemocolitis should include modern diagnostic methods, if necessary, additional instrumental studies and specialist consultations to exclude inflammatory bowel diseases.

Testing Evolutionary Models with Red Supergiant and Wolf–Rayet Populations

Despite the many successes that modern massive star evolutionary theory has enjoyed, reproducing the apparent trend in the relative number of red supergiants (RSGs) and Wolf–Rayet (WR) stars has remained elusive. Previous estimates show the RSG/WR ratio decreasing strongly with increasing metallicity. However, the evolutionary models have always predicted a relatively flat distribution for the RSG/WR ratio. In this paper we reexamine this issue, drawing on recent surveys for RSGs and WRs in the Magellanic Clouds, M31, and M33. The RSG surveys have used Gaia astrometry to eliminate foreground contamination and have separated RSGs from asymptotic giant branch stars using near-infrared colors. The surveys for WRs have utilized interference-filter imaging, photometry, and image subtraction techniques to identify candidates, which have then been confirmed spectroscopically. After carefully matching the observational criteria to the models, we now find good agreement in both the single-star Geneva and binary BPASS models with the new observations. The agreement is better when we shift the RSG effective temperatures derived from J − Ks photometry downwards by 200 K in order to agree with the Levesque TiO effective temperature scale. In an appendix we also present a source list of RSGs for the SMC which includes effective temperatures and luminosities derived from near-infrared 2MASS photometry, in the same manner as used for the other galaxies.

Quercetin effects on adaptive immune response in experimental periodontitis of bacterial-immune genesis

In the article was studied the effects of flavonol quercetin on indices of adaptive immune response in experimental animals on the 14th day of the experimental bacterial-immune periodontitis development. Indices of immune protection were determined by the relative number of lymphocytes with CD3+, CD4+, CD8+, CD19+, CD16+ and immunoregulatory index (CD4+ / CD8+) in intact animals and on the 14th day of inflammatory process development in periodontal tissues as well as the therapeutic effects of flavonol quercetin. As a result of the study, characterized changes associated with the activity of both the cell-mediated and humoral-immune response were found, both in the development of experimental periodontitis, and apply of flavonol. In particular, there was an increase in the animal’s blood relative amount of CD8+, CD16+ cells on the 14th day, and content of CD3+, CD4+, CD19+ was decreased. In this case, the immunoregulatory index (CD4+ / CD8+) as an important index of immunological activity was decreased. The apply of flavonol quercetin in the period development of bacterial-immune periodontitis animals functional activity of the T-cell line of the immune system was increased, as evidenced percentage increase of B- and T-cells due to T-helper cells decrease as well as T-killers content during this period of inflammatory reaction in the periodontal complex, in comparison with animals, which were not treated.