Characterisation of newly emerged isolates of classical swine fever virus in China, 2014–2015

Abstract Introduction: In 2014–2015, the epidemic of classical swine fever (CSF) occurred in many large-scale pig farms in different provinces of China, and a subgenotype 2.1d of CSF virus (CSFV) was newly identified. Material and Methods: The phylogenetic relationship, genetic diversity, and epidemic status of the 2014–2015 CSFV isolates, 18 new CSFV isolates collected in 2015, and 43 other strains isolated in 2014–2015 were fully analysed, together with 163 CSFV reference isolates. Results: Fifty-two 2014–2015 isolates belonged to subgenotype 2.1d and nine other isolates belonged to subgenotype 2.1b. The two subgenotype isolates showed unique molecular characteristics. Furthermore, the 2.1d isolates were found to possibly diverge from 2.1b isolates. Conclusion: This study suggests that the Chinese CSFVs will remain pandemic.

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Deciphering the emergence, genetic diversity and evolution of classical swine fever virus

Scientific Reports ◽

10.1038/s41598-017-18196-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 14

Author(s):

Liliam Rios ◽

Liani Coronado ◽

Dany Naranjo-Feliciano ◽

Orlando Martínez-Pérez ◽

Carmen L. Perera ◽

...

Keyword(s):

Genetic Diversity ◽

Classical Swine Fever Virus ◽

Classical Swine Fever ◽

Fever Virus

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Genetic diversity and molecular characterization of classical swine fever virus envelope protein genes E2 and Erns circulating in Vietnam from 2017 to 2019

Infection Genetics and Evolution ◽

10.1016/j.meegid.2021.105140 ◽

2021 ◽

pp. 105140

Author(s):

Ngoc Hai Nguyen ◽

Binh Nguyen Thi Phuong ◽

Duy Do Tien ◽

Trung Quan Nguyen ◽

Duyen Nguyen Thi My ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Characterization ◽

Classical Swine Fever Virus ◽

Envelope Protein ◽

Classical Swine Fever ◽

Fever Virus ◽

Virus Envelope ◽

Virus Envelope Protein

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Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x1999000300006 ◽

1999 ◽

Vol 19 (3-4) ◽

pp. 123-127 ◽

Cited By ~ 5

Author(s):

Julio Cesar Muñoz Paredes ◽

Liliane Guimarães Oliveira ◽

Alexandre de Carvalho Braga ◽

Iara Maria Trevisol ◽

Paulo Michel Roehe

Keyword(s):

Classical Swine Fever Virus ◽

Predictive Value ◽

Neutralizing Antibodies ◽

Large Scale ◽

Classical Swine Fever ◽

Fever Virus ◽

Serological Diagnosis ◽

Immunoperoxidase Staining ◽

Serum Samples ◽

Swine Serum

An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.

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