A large field-of-view metasurface for complex-amplitude hologram breaking numerical aperture limitation

AbstractOwing to the potential to manipulate simultaneously amplitude and phase of electromagnetic wave, complex-amplitude holographic metasurfaces (CAHMs) can achieve improved image-reconstruction quality compared with amplitude-only and phase-only ones. However, prevailing design methods based on Huygens–Fresnel theory for CAHMs, e.g., Rayleigh–Sommerfeld diffraction theory (RSDT), restrict acquisition of high-precision reconstruction in a large field of view (FOV), especially in the small numerical aperture (NA) scenario. To this end, a CAHM consisting of Sine-shaped meta-atoms is proposed in a microwave region, enabled by a novel complex amplitude retrieval method, to realize large FOV holograms while breaking the large NA limitation. Calculations and full-wave simulations demonstrate that the proposed method can achieve superior-quality holograms, even for nonparaxial holograms in a relatively small NA scenario, thus improving FOV and aperture utilization efficiency of CAHMs. The reconstruction comparison of a complex multi-intensity field distribution between CAHM prototypes designed by our method and by RSDT further confirms this point. We also compare both theoretically and experimentally the CAHM by our method with the phase-only metasurface by weighted Gerchberg–Saxton algorithm. Superior-quality holograms with suppressed background noise and relieved deformation, promised by the extra amplitude manipulation freedom, is witnessed. Finally, due to its wavelength irrelevance, the proposed method is applicable to the entire spectrum, spanning from microwave to optics.

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A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging

eLife ◽

10.7554/elife.14472 ◽

2016 ◽

Vol 5 ◽

Cited By ~ 226

Author(s):

Nicholas James Sofroniew ◽

Daniel Flickinger ◽

Jonathan King ◽

Karel Svoboda

Keyword(s):

Neural Activity ◽

Single Cells ◽

Numerical Aperture ◽

Random Access ◽

Field Of View ◽

Large Field ◽

Excitation Wavelength ◽

Scanning System ◽

Brain Areas

Imaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within a volume spanning multiple brain areas (∅ 5 mm x 1 mm cylinder). 2p-RAM resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast three-dimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume. We illustrate the use of the 2p-RAM by imaging neural activity in multiple, non-contiguous brain areas in transgenic mice expressing protein calcium sensors.

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A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging

10.1101/055947 ◽

2016 ◽

Cited By ~ 3

Author(s):

N. J. Sofroniew ◽

D. Flickinger ◽

J. King ◽

K. Svoboda

Keyword(s):

Neural Activity ◽

Single Cells ◽

Numerical Aperture ◽

Random Access ◽

Field Of View ◽

Large Field ◽

Excitation Wavelength ◽

Scanning System ◽

Brain Areas

AbstractImaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (< 1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within a volume spanning multiple brain areas (Ø 5 mm × 1 mm cylinder). 2p-RAM resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast threedimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume. We illustrate the use of the 2p-RAM by imaging neural activity in multiple, non-contiguous brain areas in transgenic mice expressing protein calcium sensors.

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A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics

10.1101/2020.04.07.030569 ◽

2020 ◽

Cited By ~ 5

Author(s):

Etai Sapoznik ◽

Bo-Jui Chang ◽

Jaewon Huh ◽

Robert J. Ju ◽

Evgenia V. Azarova ◽

...

Keyword(s):

High Resolution ◽

Numerical Aperture ◽

Light Sheet ◽

Membrane Dynamics ◽

Field Of View ◽

Large Field ◽

Endoplasmic Reticulum Membrane ◽

High Resolution Imaging ◽

Light Sheet Microscopy ◽

Resolution Imaging

AbstractWe present an Oblique Plane Microscope that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of Lattice Light-Sheet Microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.

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High numerical aperture large field-of-view metalens imaging

High Contrast Metastructures X ◽

10.1117/12.2578130 ◽

2021 ◽

Author(s):

Junjie Hu ◽

Weijian Yang

Keyword(s):

Numerical Aperture ◽

Field Of View ◽

Large Field ◽

High Numerical Aperture

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A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

eLife ◽

10.7554/elife.57681 ◽

2020 ◽

Vol 9 ◽

Cited By ~ 1

Author(s):

Etai Sapoznik ◽

Bo-Jui Chang ◽

Jaewon Huh ◽

Robert J Ju ◽

Evgenia V Azarova ◽

...

Keyword(s):

High Resolution ◽

Numerical Aperture ◽

Light Sheet ◽

Membrane Dynamics ◽

Field Of View ◽

Large Field ◽

Endoplasmic Reticulum Membrane ◽

High Resolution Imaging ◽

Light Sheet Microscopy ◽

Resolution Imaging

We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.

Download Full-text