scholarly journals Effect of oil cakes and garlic aqueousbased formulations of trichoderma viride on management of meloidogyne incognita in chilli

2018 ◽  
Vol 3 (1) ◽  
pp. 214-219
Author(s):  
A. Nirosha ◽  
S. Rajeshkanna ◽  
G. Mikunthan

Abstract Root-knot nematode, Meloidogyne incognita (Kofoid and White) is a major threat to chilli (Capsicum annum) cultivation, by forming root galls and subsequent wilting. These nematodes live in soil, roots debris and reported surviving in other Solanaceae crops. Chemical application of nematicides cause impact on environment, therefore biocontrol using antagonistic fungi is desired to tackle this problem. This research is aimed to evaluate the effect of Trichoderma viride and its formulations on management of M. incognita. Oil cakes such as neem (Azadiracta indica), gingelly (Sesamum indicum), Mahua (Madhuca longifolia) and garlic (Allium sativum) extracts were used to evaluate the growth and sporulation of T. viride. Chilli variety MI-2 was used. Among the four preparations, neem oil cake recorded high spore yield of 1.75 ×107 spores/ml. Similarly gingelly oil cake and garlic produced the spore yields of 1.57 ×107 spores/ml and 1.368 ×107 spores/ml, respectively. Comparatively Mahua oil cake obtained low spore yield of 7.87×106 spores/ml. Plant growth was significant (P<0.05) in the application of neem oil cake formulation (30.42 cm). Extent of galling was significantly low with garlic (9.00) and neem (9.25). These results confirmed that the formulations of neem, gingelly oil cake extracts and garlic aqueous extract stimulated the chilli plant growth, productivity and reduced the nematode infestation. Above all, neem oil cake and garlic are the best formulations that can be used to manage M. incognita

Author(s):  
Manaswini Mahapatra ◽  
Dhirendra Kumar Nayak ◽  
Pranaya Pradhan ◽  
Antaryami Mishra

The study was aimed to control nematodes in ginger crop in a sustainable way through inoculation of bio-agent, Trichoderma viride. The outcome of our research revealed that Trichoderma viride alone (T2) was the most effective treatment among all. This bio-agent has lowered the root-knot nematode populations by suppressing the disease intensity and responsible for enhancement of plant growth, increases the availability of nutrients to host plant. Meloidogyne incognita alone (T1) infected rhizomes resulted in highest reduction of calcium content amounting 2.26 and 2.43 mg/100g dry weight whereas maximum content was recorded in Trichoderma viride alone (T2) inoculated rhizomes as 2.84 and 3.08 mg 100g-1 dry weight in suravi and suprabha over control (T7) respectively. Trichoderma viride inoculated 15 days prior to Meloidogyne incognita (TV→MI) was found to be most efficient one among all combined treatments. Susceptibility towards nematode induced more nutrient deposition in rhizomes of ginger crop due to poor translocation process through xylem.


Author(s):  
P. Saravanan ◽  
N. Ilavarasan ◽  
A. Karthikeyan ◽  
B. Padmanaban

A pot culture experiment was performed to study the effect of native promising biocontrol agents and neem cake separately and in combination against root-knot nematode Meloidogyne incognita infecting banana cv. Robusta during 2016-2017. Treatments treated with bioagents and neem cake enhanced plant growth and root characters and suppressed root gall development in banana plant as compared to nematode alone and untreated control treatments. Among the various treatments, the combined application of Pseudomonas fluorescens @ 10 g/plant + Trichoderma viride @ 10 gm/plant + neem cake @ 100 gm/plant resulted in maximum increase of plant height (38.8 cm), number of leaves (7), pseudostem girth (15 cm), root length (25.6 cm), highest number of healthy roots (17.0), poor number of infected roots (3.3), lowest root gall index (1 scale) and significantly reduced root-knot nematode population both soil (55.0 nos) and roots (90.0 nos). The application of single bioagent either P.fluorescens @ 10 gm/plant or T.viride @ 10 gm/plant along with neem cake were also recorded highest plant growth parameters and poor nematode infestation when compared to nematode alone treated control plant.


2021 ◽  
Vol 12 (5) ◽  
pp. 286-294
Author(s):  
Poornata Jena ◽  
◽  
N. K. Sahoo ◽  
J. K. Mahalik ◽  
◽  
...  

A pot experiment was carried out in the net house of Department of Nematology, OUAT, Bhubaneswar, Odisha, India during June to August, 2017 on the application of oilcakes (mustard cake and neem cake) and bio-agents (Trichoderma viride, Glomus fasciculatum, Rhizobium leguminosarum) each alone and in combination for the management of root knot nematode (Meloidogyne incognita) in green gram. Result of the experiment indicated that soil application of mustard or neem cake @ 50 g m-2 with AM fungus (Glomus fasciculatum) @ 5 g m-² and seed treatment of Rhizobium @ 25 g kg-1 of green gram seed declined the root knot nematode population, number of galls plant-1, number of eggmass plant-1and root knot index with corresponding increase of plant growth parameters and chlorophyll content in green gram plant as compared to other treatments and untreated check. But integration of mustard cake @ 50 g m-2 at 2 weeks prior to sowing with AM fungus @ 5 g m-2 at 10 days before sowing and seed treatment of Rhizobium @ 25 g kg-1 green gram seed exhibited the lowest M. incognita population 200 cc soil-1 (153.33 J2), number of galls plant-1 (7.0), number of eggmass plant-1 (2.0) and root knot index (2.0) reflecting enhancement of plant growth parameters, number of pods (206.67%), number of nodules (691.17%) over untreated check. This integrated management module also recorded maximum increase in the availability of NPK content in soil and chlorophyll content as compared to other treatments.


HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1013D-1013
Author(s):  
Yan Chen ◽  
Donald Merhaut ◽  
J. Ole Becker

Nitrogen (N) fertilization is critical for successful production of cut flowers in a hydroponic system. In this study, two sunflower cultivars: single-stand `Mezzulah' and multi-stand `Golden Cheer' were grown under two N fertilization rates: 50 mg·L-1 and 100 mg·L-1 in a recirculating hydroponic system. At the same time, `Mezzulah' sunflowers were biologically stressed by exposing each plant to 2000 second-stage juveniles of the plant parasitic nematode Meloidogyne incognita, race 1. The experiment was conducted in May and repeated in Sept. 2004, and plant growth and flower quality between control and nematode-infested plants were compared at the two N rates. The two cultivars responded differently to fertilization treatments. With increasing N rate, the dry weight of `Mezzulah' increased, while that of `Golden Cheer' decreased. Flower size and harvest time were significantly different between the two cultivars. However, N had no effect on flower quality and harvest time. Flower quality rating suggests that quality cut stems can be obtained with 50 mg·L-1 N nutrient solution. Nematode egg count suggests that plants in the nematode treatment were successfully infested with Meloidogyne incognita, however, no significant root galling was observed, and plant growth and flower quality were not affected by nematode infestation.


2021 ◽  
Author(s):  
Jaydeep Ashok Patil ◽  
Saroj Yadav ◽  
Sewak Ram

Abstract Background: Complex disease caused by root-knot nematode and Fusarium fungus in cucumber is the most destructive disease under polyhouse conditions. The study was conducted to determine the potential of biocontrol agents viz. Trichoderma viride, Pseudomonas fluorescence, Purpureocillium lilacinum against root-knot nematode, Meloidogyne incognita and Fusarium oxysporum f. sp. cucumerinum disease complex on cucumber.Results: All bioagents were taken @ 0.3 and 0.5 g/kg soil and liquid formulation of bioagents, @ 0.5 and 1 ml/ kg soil, Chemical checks as well as untreated check were also maintained. The bio-agents were mixed with the potted soil treatment wise. All the treatments significantly improved the plant growth parameter, viz., shoot length, root length, fresh shoot weight, fresh root weight, dry shoot weight and dry root weight as compared to untreated check. However, maximum improvement in plant growth parameter was recorded in case of carbofuran followed by higher dose of liquid formulation of bioagents. Among bioagents, liquid formulation of bioagents was significantly improved the plant growth parameter, viz., shoot length (147.3), fresh shoot weight (55.6), dry shoot weight (22.51) and dry root weight (4.50) and most effective in suppressing root knot nematode galling (43) and final population in soil (131) and fungus wilt incidence (25 %) at 30th day of after germination followed by P. lilacinum T. viride and P. fluorescence. Conclusion: The results suggest that the liquid formulation of bioagents was more effective in suppression of root-knot nematode and fungus complex disease than the powder formulations of bioagents. More studies should be needed in future to evaluate the efficacy of bioagents as seed treatments and soil applications under field conditions.


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