scholarly journals Molecular Characterization of Lates niloticus (Perciformes, Latidae) Populations from three Nigerian Waterbodies using Random Amplified Polymorphic DNA and Microsatellite Markers

2017 ◽  
Vol 51 (1) ◽  
pp. 31-36
Author(s):  
K. E. Ogbuebunu ◽  
M. O. Awodiran
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Random Amplified Polymorphic Dna ◽  
Population Level ◽  
Effective Number ◽  
Lates Niloticus ◽  
The Mean

Abstract Thirty Lates niloticus (Linnaeus, 1758) from three Nigerian waterbodies were genotyped on six RAPD primers and five microsatellites loci. RAPD revealed that effective number of alleles (AE) at population level per locus was within the range of 1.641 ± 0.066 to 1.645 ± 0.041 while the mean number of alleles (AN) across populations equals 2.000. Characterization on five microsatellites loci revealed genetic diversity within and among studied populations. Observed heterozygousity (HO) was within the range of 0.317 ± 0.335 to 0.523 ± 0.315 while expected heterozygousity (HE) was within the range of 0.414 ± 0.306 to 0.715 ± 0.097. Proportion of differentiation (FST) within populations was 0.236. Overall gene flow (Nm) among populations equals 0.806. This study established the successful use of RAPD and microsatellite as tools for studying population structure of fish species, especially L. niloticus. Thus, it can be concluded that L. niloticus in the three (3) sampled Nigerian waterbodies is undergoing evolution.

Schistosome genetic diversity: the implications of population structure as detected with microsatellite markers

Parasitology ◽  
2002 ◽  
Vol 125 (7) ◽  
pp. S51-S59 ◽  
Author(s):  
J. CURTIS ◽  
R. E. SORENSEN ◽  
D. J. MINCHELLA
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variation ◽  
Molecular Markers ◽  
Microsatellite Markers ◽  
Natural Populations ◽  
Mitochondrial Marker ◽  
Tropical Regions ◽  
Blood Flukes

Blood flukes in the genus Schistosoma are important human parasites in tropical regions. A substantial amount of genetic diversity has been described in populations of these parasites using molecular markers. We first consider the extent of genetic variation found in Schistosoma mansoni and some factors that may be contributing to this variation. Recently, though, attempts have been made to analyze not only the genetic diversity but how that diversity is partitioned within natural populations of schistosomes. Studies with non-allelic molecular markers (e.g. RAPDs and mtVNTRs) have indicated that schistosome populations exhibit varying levels of gene flow among component subpopulations. The recent characterization of microsatellite markers for S. mansoni provided an opportunity to study schistosome population structure within a population of schistosomes from a single Brazilian village using allelic markers. Whereas the detection of population structure depends strongly on the type of analysis with a mitochondrial marker, analyses with a set of seven microsatellite loci consistently revealed moderate genetic differentiation when village boroughs were used to define parasite subpopulations and greater subdivision when human hosts defined subpopulations. Finally, we discuss the implications that such strong population structure might have on schistosome epidemiology.

Allozyme variation and population structure of Carex humilis var. nana (Cyperaceae) in Korea

2001 ◽  
Vol 79 (4) ◽  
pp. 457-463 ◽  
Author(s):  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variation ◽  
North American ◽  
Population Level ◽  
Allozyme Variation ◽  
Population Divergence ◽  
Geographic Ranges ◽  
The Mean ◽  
Allozyme Loci

Genetic diversity and population structure of 22 Carex humilis var. nana Ohwi (Cyperaceae) populations in Korea were determined using genetic variation at 23 allozyme loci. This is a long-lived herbaceous species with a widespread distribution in eastern Asia. The 12 enzymes revealed 23 putative loci, of which 11 were polymorphic (47.8%). Genetic diversity at the varietal level and at the population level was 0.131 and 0.118, respectively. Total genetic diversity (HT = 0.274) and within population genetic diversity (HS = 0.256) were high, whereas the extent of the population divergence was relatively low (GST = 0.068). An indirect estimate of the number of migrants per generation (Nm = 3.42) indicated that gene flow was high among Korean populations. Wide geographic ranges, perennial herbaceous nature, and the persistence of multiple generations are associated with the high level of genetic variation. A distinct difference between Asian and North American Carex is shown in the proportion of genetic variation (GST) (p < 0.001). The mean GST of Asian Carex was estimated as 0.056; thus, only 5.6% of genetic variability was distributed among populations, whereas the mean GST of North American Carex was estimated as 19.5% (3.5 times higher). It is probable that the geographical distance between population pairs and presence or absence of glacial history may play roles in the substantial difference between both groups.Key words: Carex humilis var. nana, genetic diversity, population structure.

Characterization of the Genetic Diversity and Population Structure for the Yellow Cattle in Taiwan Based on Microsatellite Markers

2014 ◽  
Vol 25 (4) ◽  
pp. 234-249 ◽  
Author(s):  
Po-An Tu ◽  
Der-Yuh Lin ◽  
Guang-Fu Li ◽  
Jan-Chi Huang ◽  
De-Chi Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Yellow Cattle

scholarly journals Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

2017 ◽  
Vol 62 (No. 5) ◽  
pp. 219-225 ◽  
Author(s):  
R. Di ◽  
Q.Y. Liu ◽  
F. Xie ◽  
W.P. Hu ◽  
X.-Y. Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Principal Component ◽  
Principal Component Analysis Plot ◽  
Animal Conservation ◽  
Mean Values ◽  
Expected Heterozygosity ◽  
The Mean ◽  
Near Future

China had the largest population of raising donkeys in the world, however the number of Chinese indigenous donkey decreased dramatically due to the increase of agriculture mechanization in the last century. The species has still been important in China because of its edible and medical value, therefore the survey on its genetic diversity in China is necessary for its conservation and utilization. In this study, 15 microsatellite markers were used to evaluate genetic diversity and population structure of five Chinese indigenous donkey breeds. The mean values of expected heterozygosity, allelic richness, and total number of alleles for all the tested Chinese donkeys were 0.70, 6.04, and 6.28 respectively, suggesting that the genetic diversity of Chinese indigenous donkeys is rich. The Bayesian analysis and principal component analysis plot yielded the same clustering result, which revealed that Guanzhong donkey was the most differentiated breed in all detected samples, and Jinnan (JN) and Guangling (GL) were genetically closed together. Additionally, our results indicated that the heterozygote deficit was severe in two Chinese indigenous donkey breeds (GL and JN), and it warned us that animal conservation activities on this species should be considered carefully in near future.

Characterization of the genetic diversity and population structure of the manakin genus Antilophia through the development and analysis of microsatellite markers

2019 ◽  
Vol 160 (3) ◽  
pp. 825-830 ◽  
Author(s):  
Thainara O. Souza ◽  
Leilton W. Luna ◽  
Juliana Araripe ◽  
Mauro A. D. Melo ◽  
Weber A. G. Silva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers

Genetic diversity, population structure, and historical gene flow of Nelumbo lutea in USA using microsatellite markers

2020 ◽  
Vol 160 ◽  
pp. 103162 ◽  
Author(s):  
Md. Rabiul Islam ◽  
Ying Zhang ◽  
Zhi-Zhong Li ◽  
Hong Liu ◽  
Jin-Ming Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Nelumbo Lutea

Genetic Markers and Quantitative Genetic Variation in Medicago truncatula (Leguminosae): A Comparative Analysis of Population Structure

Genetics ◽  
1996 ◽  
Vol 143 (4) ◽  
pp. 1795-1805 ◽  
Author(s):  
Isabelle Bonnin ◽  
Jean-Marie Prosperi ◽  
Isabelle Olivieri
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Genetic Markers ◽  
Medicago Truncatula ◽  
Random Amplified Polymorphic Dna ◽  
Population Level ◽  
Components Of Variance ◽  
Quantitative Characters ◽  
Marker Loci ◽  
The Face

Abstract Two populations of the selfing annual Medicago truncatula Gaertn. (Leguminoseae), each subdivided into three subpopulations, were studied for both metric traits (quantitative characters) and genetic markers (random amplified polymorphic DNA and one morphological, single-locus marker). Hierarchical analyses of variance components show that (1) populations are more differentiated for quantitative characters than for marker loci, (2) the contribution of both within and among subpopulations components of variance to overall genetic variance of these characters is reduced as compared to markers, and (3) at the population level, within population structure is slightly but not significantly larger for markers than for quantitative traits. Under the hypothesis that most markers are neutral, such comparisons may be used to make hypotheses about the strength and heterogeneity of natural selection in the face of genetic drift and gene flow. We thus suggest that in these populations, quantitative characters are under strong divergent selection among populations, and that gene flow is restricted among populations and subpopulations.

scholarly journals Genetic diversity and population structure of the narrow endemic and endangered species Heteroplexis microcephala Y. L. Chen. in China revealed by random amplified polymorphic DNA markers

2016 ◽  
Vol 68 (3) ◽  
pp. 669-675
Author(s):  
Yancai Shi ◽  
Xiao Wei ◽  
Jiqing Wei ◽  
Yongtao Li ◽  
Shengfeng Chai ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Endangered Species ◽  
Genetic Differentiation ◽  
Dna Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Population Level ◽  
Dispersal Distance ◽  
Ex Situ

Heteroplexis microcephala Y. L. Chen. is an endemic and endangered species found only in karst limestone regions in the Yangshuo County of the Guangxi Zhuang Autonomous Region in China: it is a habitat representative of species in the Heteroplexis genus. To provide basic genetic information for its conservation, in this study we evaluated the genetic variation and differentiation among six wild populations of H. microcephala by random amplified polymorphic DNA markers (RAPD). The leaves of 141 individuals were sampled. Based on 12 primers, 113 DNA fragments were generated. Genetic diversity was low at the population level (Nei?s gene diversity (h)=0.0579; Shannon information index (I)=0.0924; percentage of polymorphic bands (PPB)=23.30%), but relatively high at the species level (h=0.1701; I=0.2551; PPB=46.34%). The coefficient of genetic differentiation based on Nei?s genetic diversity analysis (0.6661) was high, indicating that there was significant genetic differentiation among populations, which was confirmed by AMOVA analysis exhibiting population differentiation among populations of 68.77%. Low gene flow among populations (0.2507) may result from several factors, such as a harsh pollination environment, population isolation and low seed dispersal distance. Limited gene flow and self-compatibility are the primary reasons for the high genetic differentiation observed for this species. We propose the collection of seeds from more populations with fewer individuals and core populations for ex situ conservation and suggest methods to increase seed germination rates.

scholarly journals Isolation and characterization of ten new microsatellite markers in Machaerium villosum Vogel (Fabaceae)

Hoehnea ◽  
2014 ◽  
Vol 41 (1) ◽  
pp. 77-80
Author(s):  
João Giudice-Neto ◽  
Rafael Flora Ramos ◽  
Evandro Marsola de Moraes ◽  
Márcio José da Silva ◽  
Vera Nisaka Solferini
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Southeastern Brazil ◽  
Atlantic Rain Forest ◽  
High Genetic Diversity ◽  
Isolation And Characterization ◽  
Minas Gerais State ◽  
The Mean ◽  
Significant Linkage

Machaerium villosum is an important tree species from Southeastern Brazil. We report hereby 10 new microsatellite markers to investigate the structure and genetic diversity of this species. Ninety-seven alleles were detected in 60 specimens from a natural population in Minas Gerais State. High genetic diversity has been found. The mean observed and expected heterozygosities were 0.771 and 0.802, respectively. One locus showed significant Hardy-Weinberg departure and five loci combinations showed significant linkage disequilibrium. These 10 new microsatellite loci will be used to evaluate the genetic diversity of this species in order to understand the fragmentation effects of the Brazilian Atlantic Rain Forest.

Sign in / Sign up

Export Citation Format

Share Document