Population structure, gene flow and genetic diversity analyses based on agro-morphological traits and microsatellite markers within cultivated and wild germplasms of okra [Abelmoschus esculentus (L.) Moench.]

2021 ◽  
Author(s):  
Anjan Das ◽  
Ramesh Kumar Yadav ◽  
Harshwardhan Choudhary ◽  
Suman Lata ◽  
Saurabh Singh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Morphological Traits ◽  
Abelmoschus Esculentus

scholarly journals Molecular Characterization of Lates niloticus (Perciformes, Latidae) Populations from three Nigerian Waterbodies using Random Amplified Polymorphic DNA and Microsatellite Markers

2017 ◽  
Vol 51 (1) ◽  
pp. 31-36
Author(s):  
K. E. Ogbuebunu ◽  
M. O. Awodiran
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Random Amplified Polymorphic Dna ◽  
Population Level ◽  
Effective Number ◽  
Lates Niloticus ◽  
The Mean

Abstract Thirty Lates niloticus (Linnaeus, 1758) from three Nigerian waterbodies were genotyped on six RAPD primers and five microsatellites loci. RAPD revealed that effective number of alleles (AE) at population level per locus was within the range of 1.641 ± 0.066 to 1.645 ± 0.041 while the mean number of alleles (AN) across populations equals 2.000. Characterization on five microsatellites loci revealed genetic diversity within and among studied populations. Observed heterozygousity (HO) was within the range of 0.317 ± 0.335 to 0.523 ± 0.315 while expected heterozygousity (HE) was within the range of 0.414 ± 0.306 to 0.715 ± 0.097. Proportion of differentiation (FST) within populations was 0.236. Overall gene flow (Nm) among populations equals 0.806. This study established the successful use of RAPD and microsatellite as tools for studying population structure of fish species, especially L. niloticus. Thus, it can be concluded that L. niloticus in the three (3) sampled Nigerian waterbodies is undergoing evolution.

Genetic diversity, population structure, and historical gene flow of Nelumbo lutea in USA using microsatellite markers

2020 ◽  
Vol 160 ◽  
pp. 103162 ◽  
Author(s):  
Md. Rabiul Islam ◽  
Ying Zhang ◽  
Zhi-Zhong Li ◽  
Hong Liu ◽  
Jin-Ming Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Nelumbo Lutea

First Records on Genetic Diversity and Population Structure of Algerian Peanut (Arachis hypogaea) Using Microsatellite Markers

2021 ◽  
Author(s):  
Hanène Djeghim ◽  
Ines Bellil ◽  
Rima Hind Boudchicha ◽  
Ali Boumegoura ◽  
Douadi Khelifi
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Arachis Hypogaea

scholarly journals Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Workia Ahmed ◽  
Tileye Feyissa ◽  
Kassahun Tesfaye ◽  
Sumaira Farrakh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Information Content ◽  
Date Palm ◽  
Polymorphic Information Content ◽  
Phoenix Dactylifera ◽  
Mean Value ◽  
Date Palms ◽  
Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

Genetic diversity, population structure and gene flow pattern among populations of Lasiurus sindicus Henr. - an endemic, C4 grass of Indian Thar desert

Plant Gene ◽  
2020 ◽  
Vol 21 ◽  
pp. 100206 ◽  
Author(s):  
Bhuwnesh Goswami ◽  
Rekha Rankawat ◽  
Wahlang Daniel Regie ◽  
Bhana Ram Gadi ◽  
Satyawada Rama Rao
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Flow Pattern ◽  
Thar Desert ◽  
C4 Grass

scholarly journals Identification of a new Indian camel germplasm by microsatellite markers based genetic diversity and population structure of three camel populations

2020 ◽  
Vol 27 (7) ◽  
pp. 1699-1709
Author(s):  
Rekha Sharma ◽  
Sonika Ahlawat ◽  
Himani Sharma ◽  
Ved Prakash ◽  
Shilpa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers

Genetic diversity and population structure of Korean wild soybean ( Glycine soja Sieb. and Zucc.) inferred from microsatellite markers

2017 ◽  
Vol 71 ◽  
pp. 87-96 ◽  
Author(s):  
Muhammad Amjad Nawaz ◽  
Seung Hwan Yang ◽  
Hafiz Mamoon Rehman ◽  
Faheem Shehzad Baloch ◽  
Jeong Dong Lee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Glycine Soja ◽  
Wild Soybean

Genetic diversity and population structure assessment of Chinese Senna obtusifolia L. by molecular markers and morphological traits of seed

2017 ◽  
Vol 40 (1) ◽  
Author(s):  
Renjun Mao ◽  
Pengguo Xia ◽  
Jingling Liu ◽  
Xin Li ◽  
Ruilian Han ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Senna Obtusifolia ◽  
Structure Assessment

scholarly journals Population Genetics of the Red Rock Lobster,  Jasus edwardsii

2021 ◽  
Author(s):  
◽  
Luke Thomas
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
New Zealand ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Rocky Reef ◽  
Rock Lobster ◽  
Wide Range ◽  
Jasus Edwardsii ◽  
Polymorphic Microsatellite

<p>Understanding patterns of gene flow across a species range is a vital component of an effective fisheries management strategy. The advent of highly polymorphic microsatellite markers has facilitated the detection of fine-scale patterns of genetic differentiation at levels below the resolving power of earlier techniques. This has triggered the wide-spread re-examination of population structure for a number of commercially targeted species. The aims of thesis were to re-investigate patterns of gene flow of the red rock lobster Jasus edwardsii throughout New Zealand and across the Tasman Sea using novel microsatellite markers. Jasus edwardsii is a keystone species of subtidal rocky reef system and supports lucrative export markets in both Australia and New Zealand. Eight highly polymorphic microsatellite markers were developed from 454 sequence data and screened across a Wellington south coast population to obtain basic diversity indices. All loci were polymorphic with the number of alleles per locus ranging from 6-39. Observed and expected heterozygosity ranged from 0.563-0.937 and 0.583-0.961, respectively. There were no significant deviations from Hardy-Weinberg equilibrium following standard Bonferroni corrections. The loci were used in a population analysis of J. edwardsii that spanned 10 degrees of latitude and stretched 3,500 km across the South Pacific. The analysis rejected the null-hypothesis of panmixia based on earlier mDNA analysis and revealed significant population structure (FST=0.011, RST=0.028) at a wide range of scales. Stewart Island was determined to have the highest levels of genetic differentiation of all populations sampled suggesting a high degree of reproductive isolation and self-recruitment. This study also identified high levels of asymmetric gene flow from Australia to New Zealand indicating a historical source-sink relationship between the two countries. Results from the genetic analysis were consistent with results from oceanographic dispersal models and it is likely that the genetic results reflect historical and contemporary patterns of Jasus edwardsii dispersal and recruitment throughout its range.</p>

scholarly journals Genetic Diversity and Population Structure of Bermudagrass [Cynodon dactylon (L.) Pers.] along Latitudinal Gradients and the Relationship with Polyploidy Level

Diversity ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 135 ◽  
Author(s):  
Jingxue Zhang ◽  
Miaoli Wang ◽  
Zhipeng Guo ◽  
Yongzhuo Guan ◽  
Jianyu Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Flow ◽  
Population Genetic ◽  
Cynodon Dactylon ◽  
Latitudinal Gradients ◽  
Wind Pollination ◽  
Ploidy Levels ◽  
Sexual And Asexual Reproduction ◽  
Genetic Pattern

Understanding the population genetic pattern and process of gene flow requires a detailed knowledge of how landscape characteristics structure populations. Although Cynodon dactylon (L.) Pers. (common bermudagrass) is widely distributed in the world, information on its genetic pattern and population structure along latitudinal gradients is limited. We tried to estimate the genetic diversity and genetic structure of C. dactylon along a latitudinal gradient across China. Genetic diversity among different ploidy levels was also compared in the study. The material used consisted of 296 C. dactylon individuals sampled from 16 geographic sites from 22°35′ N to 36°18′ N. Genetic diversity was estimated using 153 expressed sequence tag-derived simple sequence repeat (EST-SSR) loci. Higher within-population genetic diversity appeared at low-latitude, as well as having positive correlation with temperature and precipitation. The genetic diversity increased with the ploidy level of C. dactylon, suggesting polyploidy creates higher genetic diversity. No isolation by distance and notable admixture structure existed among populations along latitudes. Both seed dispersal (or vegetative organs) and extrinsic pollen played important roles for gene flow in shaping the spatial admixture population structure of C. dactylon along latitudes. In addition, populations were separated into three clusters according to ploidy levels. C. dactylon has many such biological characters of perennial growth, wind-pollination, polyploidy, low genetic differentiation among populations, sexual and asexual reproduction leading to higher genetic diversity, which gives it strong adaptability with its genetic patterns being very complex across all the sampled latitudes. The findings of this study are related to landscape population evolution, polyploidy speciation, preservation, and use of bermudagrass breeding.

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