The Lipid Composition of Rabies Virus

The lipid composition of the Flury HEP strain of rabies virus grown in BHK 21/C 13 cells was determined. The dried purified virus preparation contains 5.5 % neutral and 19.5 % polar lipids. Cholesterol was found to be the major neutral lipid. Phopholipids constitute 11.2 %; and glycolipids represent 4.6 % of the virus mass. The residual 3.7 % of extracted polar lipid material could not be accounted for by any known lipids. Phosphatidylethanolamine, phosphatidylcholine and sphingomyelin are the main constituents of the viral phospholipids. Hema- toside is the only ganglioside and the main glycolipid present in the virus. The glycolipids of the host cell and the virus are identical. The molar ratio of sphingolipids to glycerophospho-lipids is 0.8.

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Lipid composition of the isolated rat intestinal microvillus membrane

Biochemical Journal ◽

10.1042/bj1090051 ◽

1968 ◽

Vol 109 (1) ◽

pp. 51-59 ◽

Cited By ~ 108

Author(s):

G. G. Forstner ◽

K. Tanaka ◽

K. J. Isselbacher

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Neutral Lipid ◽

Lipid Composition ◽

Plasma Membranes ◽

Membrane Lipids ◽

Saturated Fatty Acids ◽

Polar Lipids ◽

Published Data ◽

Microvillus Membrane

1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was greatest in the membranes and closely resembled that reported for myelin. 3. Unesterified cholesterol was the major neutral lipid. However, 30% of the neutral lipid fraction was accounted for by glycerides and fatty acid. 4. Five phospholipid components were identified and measured, including phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, sphingomyelin and lysophosphatidylcholine. Though phosphatidylethanolamine was the chief phospholipid, no plasmalogen was detected. 5. In contrast with other plasma membranes in the rat, the polar lipids of the microvillus membrane were rich in glycolipid. The cholesterol:polar lipid (phospholipid+glycolipid) ratio was about 1:3 for the microvillus membrane. Published data suggest that this ratio resembles that of the liver plasma membrane more closely than myelin or the erythrocyte membrane. 6. The fatty acid composition of membrane lipids was altered markedly by a single feeding of safflower oil. Membrane polar lipids did not contain significantly more saturated fatty acids than cellular polar lipids. Differences in the proportion of some fatty acids in membrane and cellular glycerides were noted. These differences may reflect the presence of specific membrane glycerides.

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Faculty Opinions recommendation of A study of archaeal enzymes involved in polar lipid synthesis linking amino acid sequence information, genomic contexts and lipid composition.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1028632.342399 ◽

2005 ◽

Author(s):

Robert Michell

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Polar Lipid ◽

Lipid Composition ◽

Lipid Synthesis ◽

Sequence Information ◽

Amino Acid Sequence Information

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Comparative study of tissue deposition of omega-3 fatty acids from polar-lipid rich oil of the microalgae Nannochloropsis oculata with krill oil in rats

Food & Function ◽

10.1039/c4fo00591k ◽

2015 ◽

Vol 6 (1) ◽

pp. 185-191 ◽

Cited By ~ 27

Author(s):

Michael L. Kagan ◽

Aharon Levy ◽

Alicia Leikin-Frenkel

Keyword(s):

Fatty Acids ◽

Comparative Study ◽

Polar Lipid ◽

Rat Plasma ◽

Nannochloropsis Oculata ◽

Polar Lipids ◽

Omega 3 Fatty Acids ◽

Krill Oil ◽

Omega 3 ◽

Epa And Dha

An oil from micro-algae rich in EPA with no DHA and consisting of 15% polar lipids (phospholipids and glycolipids) showed equivalent uptake of EPA into rat plasma and organs as omega-3 krill oil consisting of EPA and DHA and 40% phospholipids.

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