An Improved Assay of UV-induced Thymine-Containing Dimers in Saccharomyces cerevisiae

1975 ◽  
Vol 30 (11-12) ◽  
pp. 804-810 ◽  
Author(s):  
W. W. Fäth ◽  
M. Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Highly Efficient ◽  
Layer Chromatography ◽  
Specific Labelling

Abstract The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5′-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.

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