Identification, separation, and quantitative analysis of derivatives of nucleosides and nucleotides by thin-layer chromatography on cellulose

1965 ◽  
Vol 1 (5) ◽  
pp. 262-268 ◽  
Author(s):  
T. S. Lomakina ◽  
L. I. Gus'kova ◽  
N. I. Grineva



2018 ◽  
Vol 31 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Irena Malinowska ◽  
Marek Studziński ◽  
Henryk Malinowski ◽  
Joanna Matysiak




1975 ◽  
Vol 30 (11-12) ◽  
pp. 804-810 ◽  
Author(s):  
W. W. Fäth ◽  
M. Brendel

Abstract The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5′-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.



1967 ◽  
Vol 102 (3) ◽  
pp. 815-824 ◽  
Author(s):  
J. C. Fletcher

1. A system of separation using buffered Celite columns is described that enables the pipsyl derivatives of most of the common amino acids to be separated. 2. The reaction of pipsyl chloride with several amino acids not included in previous studies has been investigated. In particular, knowledge of the acid-soluble pipsyl derivatives of arginine, histidine, lysine, tyrosine and cysteic acid has been extended. 3. Reproducible factors have been obtained that enable corrections to be applied for the breakdown of pipsylamino acids on acid hydrolysis. 4. The reaction of pipsyl chloride with peptides has been studied under various conditions. 5. The extent of the reaction between pipsyl chloride and insulin depends on the nature of the solvent-buffer system, and under the best conditions so far found is about 75% complete. 6. In an Appendix, the separation of pipsylamino acids by thin-layer chromatography is described.





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