scholarly journals Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

10.3791/62201 ◽  
2021 ◽  
Author(s):  
Sean Rogers ◽  
W. Mike Henne
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Thin Layer ◽  
Neutral Lipid ◽  
Thin Layer Chromatography ◽  
Lipid Synthesis ◽  
Metabolic Labeling ◽  
Layer Chromatography

An Improved Assay of UV-induced Thymine-Containing Dimers in Saccharomyces cerevisiae

1975 ◽  
Vol 30 (11-12) ◽  
pp. 804-810 ◽  
Author(s):  
W. W. Fäth ◽  
M. Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Highly Efficient ◽  
Layer Chromatography ◽  
Specific Labelling

Abstract The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5′-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.

Composition and Distribution of Lipids in Tissues of Bogue (Boops boops)

1985 ◽  
Vol 40 (7-8) ◽  
pp. 562-565 ◽  
Author(s):  
Vassihos M. Kapoulas ◽  
Sofia Miniadis-Meimaroglou
Keyword(s):  
Thin Layer ◽  
Neutral Lipid ◽  
Thin Layer Chromatography ◽  
Glyceryl Ether ◽  
Fatty Alcohols ◽  
Total Lipids ◽  
Triglyceride Fraction ◽  
Lipid Fractions ◽  
Layer Chromatography ◽  
Boops Boops

Abstract Total lipids from liver, head, skin and muscle of Bogue were separately isolated and their composition was investigated by a combination of analytical determinations, and column and thin layer chromatography. The major components of the neutral lipid fractions from all tissues studied were triglycerides, followed by cholesterol. The triglyceride fraction of skin and head contains significant amounts of glyceryl ether analogs. Low contents of free fatty alcohols were also identified, decreasing in the order: head, muscle, skin and liver. The major components of all phospholipid fractions was phosphatidylcholine (viz. 36-59% of total phospholipids) followed by phosphatidylethanolamine (viz. 23-34% of total phos­ pholipids). Low amounts of sphingomyelin and phosphatidylserine were also identified in all cases. All the tissues studied were found to contain plasmalogens, as well as glyceryl ether analogs in both, the depot fats and the phospholipid fractions.

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