The Electrophoretic Separation of Curved Cisplatin-Modifled DNA Fragments on Polyacrylamide Gels Is Dependent on the Voltage Gradient

1998 ◽  
Vol 53 (9-10) ◽  
pp. 921-923
Author(s):  
Julia Yaneva ◽  
Jordanka Zlatanova
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Threshold Level ◽  
Voltage Gradient ◽  
Dna Fragments ◽  
Electrophoretic Separation ◽  
Polyacrylamide Gels ◽  
Curved Dna ◽  
Electrophoretic Behavior ◽  
Param Eters

Polyacrylamide gel electrophoresis has been widely used to study DNA fragments containing sequence-dependent curvature. The anomalous electrophoretic behavior of curved DNA fragments on such gels allows their separation from straight fragments of the same length. Here we demonstrate that polyacrylamide gels can be successfully used to resolve DNA fragments modified at a single site by the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP, cisplatin) from their unmodified counterparts. However, the resolution strongly depends on the voltage gradient, being completely lost when it drops below a certain threshold level. The param eters of the electric field do not affect separation of ‘normal’ DNA fragments of comparable length.

An analysis of the proteinases ofTrichomonas vaginalisby polyacrylamide gel electrophoresis

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 1-6 ◽  
Author(s):  
G. H. Coombs ◽  
M. J. North
Keyword(s):  
Gel Electrophoresis ◽  
Trichomonas Vaginalis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cysteine Proteinases ◽  
Polyacrylamide Gels

SUMMARYThe proteinases ofTrichomonas vaginalishave been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. Seven bands of activity were detected indicating multiple proteinases. All of the enzymes were stimulated by 1 mM dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. The enzymes differed significantly, however, with respect to pH optima and relative sensitivities to inhibitors.

A COMPARATIVE STUDY OF PREPARATIONS OF OVINE LUTEINIZING HORMONE BY BIOASSAY, IMMUNODOUBLEDIFFUSION, RADIOIMMUNOASSAY, RADIORECEPTOR ASSAY AND POLYACRYLAMIDE GEL ELECTROPHORESIS

1976 ◽  
Vol 81 (2) ◽  
pp. 270-282 ◽  
Author(s):  
M. Schlamowitz ◽  
J. Cronquist ◽  
M. Esfahani ◽  
D. N. Ward
Keyword(s):  
Luteinizing Hormone ◽  
Comparative Study ◽  
Gel Electrophoresis ◽  
Binding Capacity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Radioreceptor Assay ◽  
Polyacrylamide Gel ◽  
Polyacrylamide Gels ◽  
Biological Potency

ABSTRACT Three preparations of ovine LH were compared for biological potency and by several in vitro parameters. All were found to be heterogenous by immunodoublediffusion and by electrophoresis in polyacrylamide gels. They all also showed similarities and/or differences with respect to their characteristics in immunodoublediffusion, radioimmunoassay, radioreceptor assay, gel electrophoresis and in dye-binding capacity, but in ways that preclude establishing a meaningful correlation between biopotency and the in vitro parameters or even among the in vitro parameters themselves. The implications of these findings for the use of these in vitro parameters for screening and assessing biological potencies of LH preparations and for inferring chemical and/or structural similarities between LH preparations are discussed. Aspects of polymorphism of LH, observed by electrophoresis on polyacrylamide gels, are also discussed.

Enhanced electrophoretic separation of proteins by tethered SiO2 nanoparticles in an SDS-polyacrylamide gel network

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 415-423 ◽  
Author(s):  
Sayyed Hashem Sajjadi ◽  
Hossein Ahmadzadeh ◽  
Elaheh K. Goharshadi
Keyword(s):  
Gel Electrophoresis ◽  
Heat Dissipation ◽  
Separation Efficiency ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sio2 Nanoparticles ◽  
Polyacrylamide Gel ◽  
Electrophoretic Separation ◽  
Sds Page ◽  
Separation Of Proteins ◽  
Gel Network

Tethered nanoparticles (NPs) are able to improve the separation efficiency of proteins in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) due to their capability of enhancing heat dissipation during electrophoresis and restriction of electrophoretic movement of NPs.

scholarly journals Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis

1974 ◽  
Vol 143 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Leigh A. Burgoyne ◽  
Dean R. Hewish ◽  
Jennifer Mobbs
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Nuclear Dna ◽  
Polyacrylamide Gel Electrophoresis ◽  
Dna Fragments ◽  
Two Dimensional ◽  
Digestion Time ◽  
Calcium Magnesium ◽  
Liver Chromatin ◽  
Time Courses

The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca–Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000–63000 and the smallest double-stranded class has a molecular weight of approx. 120000–150000. Studies of the substructure of the DNA fragments produced by the Ca–Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.

Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis

2004 ◽  
Vol 385 (10) ◽  
pp. 967-973 ◽  
Author(s):  
Masahiko Shiraishi ◽  
Adam J. Oates ◽  
Xu Li ◽  
Ying H. Chuu ◽  
Takao Sekiya
Keyword(s):  
Efficient Method ◽  
Gel Electrophoresis ◽  
Human Cancer ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cpg Islands ◽  
Theoretical Background ◽  
Dna Fragments ◽  
Practical Application ◽  
Gradient Gel Electrophoresis ◽  
Denaturant Gradient Gel Electrophoresis

Abstract The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed.

Electrophoretic Separation of Alkaline Phosphatase Isoenzymes: A Clinical Evaluation

1972 ◽  
Vol 17 (5) ◽  
pp. 172-175 ◽  
Author(s):  
R. R. G. Warwick ◽  
D. J. C. Shearman ◽  
I. W. Percy-Robb ◽  
A. F. Smith
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Electrophoresis ◽  
Serum Alkaline Phosphatase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Total Serum ◽  
Electrophoretic Separation ◽  
Nucleotidase Activity ◽  
Total Alkaline Phosphatase ◽  
Alkaline Phosphatase Isoenzymes ◽  
Total Alkaline

In 40 patients with a raised total serum alkaline phosphatase, polyacrylamide gel electrophoresis was used to separate the liver, bone and intestinal isoenzymes of alkaline phosphatase. The method confirmed the source of the raised alkaline phosphatase in patients with established bone or liver disease. The technique was found to be more reliable than the estimation of 5'-nucleotidase activity in the detection of a raised alkaline phosphatase of hepatic origin and was also useful when the raised total alkaline phosphatase came from two sources.

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