Novel AOPs-Based Dual-Environmental Digestion Method for Determination of Total Dissolved Nitrogen in Water

Based on a synergistic digestion method of ultraviolet combined with ozone (UV/O3), this article investigates the reaction characteristics of nitrogen-containing compounds (N-compounds) in water and the influence of ions on digestion efficiency. In this respect, a novel and efficient AOPs-based dual-environmental digestion method for the determination of total dissolved nitrogen (TDN) in waters with complex components is proposed, in the hopes of improving the detection efficiency and accuracy of total nitrogen via online monitoring. The results show that inorganic and organic N-compounds have higher conversion rates in alkaline and acidic conditions, respectively. Meanwhile, the experimental results on the influence of Cl−, CO32−, and HCO3− on the digestion process indicate that Cl− can convert to radical reactive halogen species (RHS) in order to promote digestion efficiency, but CO32− and HCO3− cause a cyclic reaction consuming numerous •OH, weakening the digestion efficiency. Ultimately, to verify the effectiveness of this novel digestion method, total dissolved nitrogen samples containing ammonium chloride, urea, and glycine in different proportions were digested under the optimal conditions: flow rate, 0.6 L/min; reaction temperature, 40 °C; pH in acidic conditions, 2; digestion time in acidic condition, 10 min; pH in alkaline conditions, 11; digestion time in alkaline conditions, 10 min. The conversion rate (CR) of samples varied from 93.23% to 98.64%; the mean CR was greater than 95.30%. This novel and efficient digestion method represents a potential alternative for the digestion of N-compounds in the routine analysis or online monitoring of water quality.

Development of Optimal Digesting Conditions for Microplastic Analysis in Dried Seaweed Gracilaria fisheri

Currently, research on the accumulation of microplastics (MPs) in the marine food web is being highlighted. An accurate and reliable digestion method to extract and isolate MPs from complex food matrices has seldom been validated. This study aimed to compare the efficacy of MP isolation among enzymatic-, oxidative-, and the combination of two digestion methods on red seaweed, Gracilaria fisheri. The dried seaweed sample was digested using three different methods under various conditions using enzymes (cellulase and protease), 30% H2O2, and a combination of enzymes and 30% H2O2. The method possessing the best digestion efficiency and polymer recovery rate of MPs was selected, and its effect on spiked plastic polymer integrity was analyzed by Raman spectroscopy. As a result, the enzymatic method rendered moderate digestion efficiency (59.3–63.7%) and high polymer recovery rate (94.7–98.9%). The oxidative method using 30% H2O2 showed high digestion efficiency (93.0–96.3%) and high polymer recovery rate (>98%). The combination method was the most effective method in terms of digestion efficiency, polymer recovery rate, and expenditure of digestion time. The method also showed no chemical changes in the spiked plastic polymers (PE, PP, PS, PVC, and PET) after the digestion process. All the spiked plastic polymers were identifiable using Raman spectroscopy.

An Innovative Digestion Method: Ultrasound-Assisted Electrochemical Oxidation for the Onsite Extraction of Heavy Metal Elements in Dairy Farm Slurry

Dairy farm slurry is an important biomass resource that can be used as a fertilizer and in energy utilization and chemical production. This study aimed to establish an innovative ultrasound-assisted electrochemical oxidation (UAEO) digestion method for the rapid and onsite analysis of the heavy metal (HM) contamination level of dairy slurry. The effects of UAEO operating parameters on digestion efficiency were tested based on Cu and Zn concentrations in a dairy slurry sample. The results showed that Cu and Zn digestion efficiency was (96.8 ± 2.6) and (98.5 ± 2.9)%, respectively, with the optimal UAEO operating parameters (digestion time: 45 min; ultrasonic power: 400 W; NaCl concentration: 10 g/L). The digestion recovery rate experiments were then operated with spiked samples to verify the digestion effect on broad-spectrum HMs. When the digestion time reached 45 min, all digestion recovery rates exceeded 90%. Meanwhile, free chlorine concentration, particle size distribution, and micromorphology were investigated to demonstrate the digestion mechanism. It was found that 414 mg/L free chorine had theoretically enough oxidative ability, and the ultrasound intervention could deal with the blocky undissolved particles attributed to its crushing capacity. The results of particle size distribution showed that the total volume and bulky particle proportion had an obvious decline. The micromorphology demonstrated that the ultrasound intervention fragmented the bulky particles, and electrochemical oxidation made irregular blocky structures form arc edge and cellular structures. The aforementioned results indicated that UAEO was a novel and efficient method. It was fast and convenient. Additionally, it ensured digestion efficiency and thus had a good application prospect.

Mechanochemical Treatment of Historical Tungsten Tailings: Leaching While Grinding for Tungsten Extraction Using NaOH

Innovative tungsten (W) extraction techniques are continually being sought because of challenges of low leaching efficiencies, despite using advanced processing units such as autoclaves operating high temperatures and pressures. Compared to conventional leaching, mechanochemical treatment improves the efficiency of leaching. Therefore, in this study, an innovative mechanochemical treatment method, referred to as leaching while grinding (LWG), was employed as a reprocessing option to optimize W recovery from historical tungsten tailings. Experiments were run using the regular two-level factorial design to screen through the four factors of stirrer speed, liquid/solid ratio, temperature, and digestion time to assess their criticality and effects in the LWG process. The stirrer speed and the liquid/solid ratio were the most critical factors in the optimization of W recovery. The maximum W recovery (91.2%) was attained at the highest stirrer speed (410 rpm), low liquid/solid ratio (0.8), long digestion time (6 h), and low leaching temperature (60 °C). The attained low leaching temperature (60 °C) was due to the mechanical activation of scheelite resulting from the simultaneous grinding and leaching. For such low- grade W material, liquid/solid ratio optimizing is critical for maintaining the digestion mixture fluidity, and for environmental and economic sustainability regarding the sodium hydroxide (NaOH) consumption, which was low.

Isolation, Purification and Characterization of Proteins in “Señorita” Banana (Musa acuminata (AAA) ‘Señorita’) Pulp with Bioactive Peptides Exhibiting Antihypertensive and Antioxidant Activities

Banana is one of the most important crops, providing multiple benefits. Although it has been widely studied for its health benefits, little information can be found about its proteins. This study determined the antihypertensive and antioxidant activities of the crude, purified, and hydrolyzed protein extracts from ‘Señorita’ banana pulp. Crude proteins were extracted using Tris-HCl buffer and purified through ammonium sulfate precipitation, dialysis, and gel filtration chromatography. The protein content of the crude, partially purified, and purified extracts were found to be 167.32, 120.45, and 28.51 μg·mL−1, respectively, with major protein having an approximate molecular weight of 15 kDa. These extracts were then subjected to enzymatic hydrolysis for release of bioactive peptides prior to ACE inhibitory and antioxidant activities determination. Among these samples, the undigested crude extract had the highest ACE inhibitory activity (85.20%). There was also an observable increase in ACE inhibition of the digested samples with increased digestion time. Meanwhile, the 3-h and 4-h crude digests had the highest DPPH radical scavenging activity with 30.82% and 34.74%, respectively. These were not significantly different from the activity of the standard, ascorbic acid. A general decrease in DPPH radical scavenging activity of the samples was observed with increased digestion time. These observations were coherent with the in silico analysis of the putative major protein, lectin, which showed that its enzymatic hydrolysis releases ACE inhibitor and antioxidant peptides.

Pepsin activity as a function of pH and digestion time on caseins and egg white proteins in static in vitro conditions

The activity of pepsin, the gastric protease, is generally considered to be negligible for pH ≥ 4, based on results obtained with few purified globular proteins. The present study aimed...

Effect of Mixture Ratio of Food Waste and Vetiver Grass on Biogas Production

The objectives of this research were to study the optimum percentage ratios of food waste and vetiver grass (Vetiveria zizaniodes (L.) Nash) for biogas production and process stability in anaerobic digestion system. The nine mixture ratios of food waste (FM) and vetiver grass (VG) were 100:0, 80:20, 75:25, 66.67:33.33, 50:50, 33.33:66.67, 25:75, 20:80 and 0:100. The biogas production was subjected to anaerobic batch with working volume of 1.8 L and had digestion time 60 d at 35 ± 2 °C. High specific methane yield of 0.30 L g-1 VS removed was obtained from the fermentation at ratio of FW:VG at 80:20 with C/N ratio of 28.20. The specific methane yield of the single digestion of food waste (100:0) and single digestion of vetiver grass (0:100) were only 0.18 and 0.11 L g-1 VS removed, respectively. It was found that the cumulative methane production of FW: VG ratio of 80:20 was increased 34.89 % and 96.93 % compared to single substrate of food waste and vetiver grass, respectively. The results also showed the highest COD, VS and TS removal with a percentage ratio of FW: VG at 80:20, while the single vetiver grass digestion was the lowest COD, VS and TS removal. VFAs/Total alkalinity ratio of all ratio of food waste to vetiver at digestion time 40 d remained in 0.0895±0.0007 to 0.1944±0.0027 were steadied for this digester. It can be concluded that co-digestion of food waste and vetiver improve the biogas yield and degradation efficiency.

Rheological and Chemical Properties of Bitumen Modified With Crumb Rubber in the Dry Process

This research aims to evaluate the performance of the hot mix asphalt modified with crumb rubber by the dry process by studying the temperature’s influence as well as the impact of the digestion time between bitumen and crumb-rubber. The residual bitumen from the drainage test and the non-modified aged bitumen are then subjected to the dynamic shear rheometer (DSR) tests at different frequencies with different temperatures and to Fourier transform infrared spectroscopy (FTIR) analysis. The results show the bitumen and the rubber interaction is directly related to the temperature and the contact time. The maximum increase in rubber mass observed here is about 42% at 195ºC for 120 minutes of contact. The decrease of the light fractions in the bitumen causes a stiffening of the latter as DSR tests show. Lastly, FTIR analysis indicates only a physical interaction.