The Oxidative Processes Induced in Cell Suspensions of Solanum Species by Culture Filtrate of Phytophthora infestans

2001 ◽  
Vol 56 (3-4) ◽  
pp. 235-244 ◽  
Author(s):  
Lidia Polkowska-Kowalczyk ◽  
Urszula Maciejewska
Keyword(s):  
Lipid Peroxidation ◽  
Phytophthora Infestans ◽  
Culture Filtrate ◽  
Peroxidase Activity ◽  
Solanum Species ◽  
Cell Suspensions ◽  
Ros Production ◽  
Polygenic Resistance ◽  
Membrane Bound ◽  
Oxidative Processes

Abstract Solanum genotypes that differ in the level of polygenic resistance to the oomycete plant pathogen Phytophthora infestans were studied for their oxidative response to culture filtrate (CF) of the pathogen. Reactive oxygen species (ROS) production, peroxidase activity and lipid peroxidation have been studied in the CF-treated cell suspensions derived from leaves of the resistant S. nigrum (nonhost) and 5. tuberosum cv. Bzura as well as from the susceptible S. tuberosum cv. Tarpan and clone H-8105. In both the resistant and susceptible cells the CF induced similar processes, but these varied with respect to the kinetics and intensity. In all cells probably the membrane-bound NADPH oxidase, was responsible for the ROS production. This process was more intensive and prolonged in the susceptible cells than in the resistant ones. The CF treatment slightly affected peroxidase activity in all cells studied. Lipid peroxidation that occurred as a consequence of the ROS accumulation was pronounced mainly in the susceptible cells. We suggest that lack of stringent control of the oxidative processes and sensitivity to the pathogen toxins may be decisive for limited polygenic resistance in potato.

Differential Responses of Potato Cell Suspensions to a Culture Filtrate of Phytophthora infestans

1997 ◽  
Vol 52 (5-6) ◽  
pp. 333-338 ◽  
Author(s):  
M. F. M. Awan ◽  
U. Maciejewska ◽  
K. Kleczkowski ◽  
B. Wielgat
Keyword(s):  
Phytophthora Infestans ◽  
Culture Filtrate ◽  
Phenylalanine Ammonia Lyase ◽  
Solanum Tuberosum L ◽  
Defense Responses ◽  
Cell Suspensions ◽  
Plant Defense Responses ◽  
Polygenic Resistance ◽  
Pal Activity ◽  
Potato Cell

Abstract General (polygenic) resistance of plant hosts to an attack by a range of pathogens is an important feature of plant defense responses against the infection. In search of biochemical markers defining this resistance, cell suspensions derived from leaves of potato (Solanum tuberosum L .) cvs. Tarpan and Bzura that are polygenically susceptible and resistant to Phytophthora infestans, respectively, were inoculated with culture filtrate (CF) of the fungus. Cell suspension of Tarpan responded to CF treatment by a higher extracellular alkalinization and more significant reduction in their viability and growth than those of the Bzura cultivar. The stimulation of phenylalanine ammonia-lyase (PAL ) activity but not of β-1,3-glucanase, was significantly higher in CF treated Bzura cells than in Tarpan ones. The obtained results suggest that sensitivity to the fungal toxins and variation of PAL activity may represent useful markers for the evaluation of polygenic resistance in potato.

scholarly journals A Culture Filtrate of Phytophthora infestans Primes Defense Reaction in Potato Cell Suspensions

2008 ◽  
Vol 98 (6) ◽  
pp. 653-658 ◽  
Author(s):  
F. Val ◽  
S. Desender ◽  
K. Bernard ◽  
P. Potin ◽  
G. Hamelin ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Culture Filtrate ◽  
Priming Effect ◽  
Cell Suspensions ◽  
Disease Suppression ◽  
Pectobacterium Atrosepticum ◽  
Defense Reactions ◽  
Potato Cell ◽  
High Concentrations ◽  
In Cells

Priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. We previously showed that application of lipopolysaccharides (LPS) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (LOX) activity. Here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. Adding 20 μg ml–1 LPS, laminarin, harpin N, or a concentrated culture filtrate (CCF) of Phytophthora infestans to cell cultures 18 h before a second elicitation with LPS did not alter the intensity of apoplastic acidification compared with a single LPS application. Conversely, high concentrations (200 or 400 μg ml–1) of LPS, laminarin, and harpin N activated LOX in cells pretreated with 1 μg ml–1 CCF, but not in cells pretreated with LPS, laminarin, or harpin N. LOX response was maximal in pretreated cells of potato cv. Bintje when the second elicitation occurred 18 to 24 h after CCF application. These results showed that LOX activation is primed in potato cells by CCF, but not by LPS, harpin N, or laminarin. Finally, bioassays showed a slightly greater reduction of rot weight in half tubers treated with CCF followed by LPS before inoculation with Pectobacterium atrosepticum than in half tubers treated with either preparation alone, indicating a priming effect of CCF on both LOX induction and disease suppression.

Changes in the initial phase of lipid peroxidation induced by elicitor from Phytophthora infestans in Solanum species

2008 ◽  
Vol 165 (18) ◽  
pp. 1929-1939 ◽  
Author(s):  
Lidia Polkowska-Kowalczyk ◽  
Jean-Luc Montillet ◽  
Jean-Pierre Agnel ◽  
Christian Triantaphylidès ◽  
Bernard Wielgat ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phytophthora Infestans ◽  
Initial Phase ◽  
Solanum Species

scholarly journals The presence of Phytophthora infestans in the rhizosphere of a wild Solanum species may contribute to off-season survival and pathogenicity

2020 ◽  
Vol 148 ◽  
pp. 103475 ◽  
Author(s):  
Ramesh R. Vetukuri ◽  
Laura Masini ◽  
Rebecca McDougal ◽  
Preeti Panda ◽  
Levine de Zinger ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Solanum Species ◽  
Wild Solanum Species ◽  
Wild Solanum

scholarly journals Identification of Three Elicitins and a Galactan-Based Complex Polysaccharide from a Concentrated Culture Filtrate of Phytophthora infestans Efficient against Pectobacterium atrosepticum

Molecules ◽  
2014 ◽  
Vol 19 (10) ◽  
pp. 15374-15390 ◽  
Author(s):  
Guillaume Saubeau ◽  
Fanny Gaillard ◽  
Laurent Legentil ◽  
Caroline Nugier-Chauvin ◽  
Vincent Ferrières ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Culture Filtrate ◽  
Pectobacterium Atrosepticum

scholarly journals Will the Bacteria Survive in the CeO2 Nanozyme-H2O2 System?

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3747
Author(s):  
Weisheng Zhu ◽  
Luyao Wang ◽  
Qisi Li ◽  
Lizhi Jiao ◽  
Xiaokan Yu ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Biomedical Applications ◽  
Antibacterial Effect ◽  
Ros Production ◽  
Ros Scavenging ◽  
Ros Scavenger ◽  
Oxide Nanoparticle ◽  
H2o2 System ◽  
Bacterial Disinfection

As one of the nanostructures with enzyme-like activity, nanozymes have recently attracted extensive attention for their biomedical applications, especially for bacterial disinfection treatment. Nanozymes with high peroxidase activity are considered to be excellent candidates for building bacterial disinfection systems (nanozyme-H2O2), in which the nanozyme will promote the generation of ROS to kill bacteria based on the decomposition of H2O2. According to this criterion, a cerium oxide nanoparticle (Nanoceria, CeO2, a classical nanozyme with high peroxidase activity)-based nanozyme-H2O2 system would be very efficient for bacterial disinfection. However, CeO2 is a nanozyme with multiple enzyme-like activities. In addition to high peroxidase activity, CeO2 nanozymes also possess high superoxide dismutase activity and antioxidant activity, which can act as a ROS scavenger. Considering the fact that CeO2 nanozymes have both the activity to promote ROS production and the opposite activity for ROS scavenging, it is worth exploring which activity will play the dominating role in the CeO2-H2O2 system, as well as whether it will protect bacteria or produce an antibacterial effect. In this work, we focused on this discussion to unveil the role of CeO2 in the CeO2-H2O2 system, so that it can provide valuable knowledge for the design of a nanozyme-H2O2-based antibacterial system.

In Vitro Reaction of Potato Micronodes to Culture Filtrate of Phytophthora Infestans

1989 ◽  
pp. 441-442 ◽  
Author(s):  
P. Crinò ◽  
R. Penuela ◽  
L. Martino ◽  
A. Sonnino ◽  
G. Ancora
Keyword(s):  
Phytophthora Infestans ◽  
Culture Filtrate

Role of an endoplasmic reticulum Ca2+-independent phospholipase A2 in oxidant-induced renal cell death

2002 ◽  
Vol 283 (3) ◽  
pp. F492-F498 ◽  
Author(s):  
Brian S. Cummings ◽  
Jane McHowat ◽  
Rick G. Schnellmann
Keyword(s):  
Lipid Peroxidation ◽  
Endoplasmic Reticulum ◽  
Subcellular Localization ◽  
Mammalian Cells ◽  
Cumene Hydroperoxide ◽  
Physiological Role ◽  
Antimycin A ◽  
Bacterial Cells ◽  
Tert Butylhydroperoxide ◽  
Membrane Bound

Phospholipase A2(PLA2) hydrolyzes the sn-2 ester bond in phospholipids, releasing a fatty acid and a lysophospholipid. Recently, a novel 85-kDa membrane-bound-Ca2+-independent PLA2 (iPLA2) was identified in insect and bacterial cells transfected with candidate PLA2 sequences. However, few data exist demonstrating a membrane-bound-iPLA2 in mammalian cells, its subcellular localization, or its physiological role. Herein, we demonstrate the expression of an 85-kDa endoplasmic reticulum (ER)-Ca2+-iPLA2 (ER-iPLA2) in rabbit renal proximal tubule cells (RPTC) that is plasmalogen selective and is inhibited by the specific Ca2+-iPLA2inhibitor bromoenol lactone (BEL). RPTC exposed to tert-butylhydroperoxide for 24 h exhibited 20% oncosis compared with 2% in controls. Inhibition of ER-iPLA2 with BEL before tert-butylhydroperoxide exposure resulted in 50% oncosis. To determine whether this effect was common to oxidants, we tested the ability of BEL to potentiate oncosis induced by cumene hydroperoxide, menadione, duraquinone, cisplatin, and the nonoxidant antimycin A. All oxidants tested produced oncosis after 24 h, and prior inhibition of ER-iPLA2 potentiated oncosis at least twofold. In contrast, inhibition of ER-iPLA2 did not alter antimycin A-induced oncosis. Lipid peroxidation increased from 1.4- to 5.2-fold in RPTC treated with BEL before oxidant exposure, whereas no change was seen in antimycin A-treated RPTC. These results are the first to demonstrate the expression and subcellular localization of an ER-iPLA2. These results also suggest that ER-iPLA2 functions to protect against oxidant-induced lipid peroxidation and oncosis.

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