scholarly journals Lichen Metabolites Modulate Hydrogen Peroxide and Nitric Oxide in Mouse Macrophages

2009 ◽  
Vol 64 (9-10) ◽  
pp. 664-672 ◽  
Author(s):  
Iracilda Z. Carlos ◽  
Marcela B. Quilles ◽  
Camila B. A. Carli ◽  
Danielle C. G. Maia ◽  
Fernanda P. Benzatti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Phenol Red ◽  
Hydrogen Peroxide Production ◽  
Griess Reagent ◽  
Mouse Macrophages ◽  
Lichen Metabolites ◽  
Oxidation Of Phenol ◽  
Peritoneal Macrophage Cells ◽  
Stimulation Of

The activities of perlatolic acid (1), atranorin (2), and lecanoric acid (3) and their derivatives, such as orsellinates and β-methyl orsellinates obtained by alcoholysis, were assessed for stimulation of the release of hydrogen peroxide and nitric oxide in cultures of peritoneal macrophage cells from mice. The hydrogen peroxide production was estimated by oxidation of phenol red, while the Griess reagent was used to determine the nitric oxide production. 1 and 4-methoxy-ethyl orsellinate (XVII) were the compounds that induced the greatest release of H2O2, whereas n-pentyl orsellinate (IV), iso-propyl orsellinate (V), sec-butyl orsellinate (VI), and XVII induced a small release of NO. These results indicate that lichen products and their derivatives have potential immune-modulating activities

Imaging of Intracellular Hydrogen Peroxide Production with HyPer upon Stimulation of HeLa Cells with EGF

2019 ◽  
pp. 85-91 ◽  
Author(s):  
Kseniya N. Markvicheva ◽  
Ekaterina A. Bogdanova ◽  
Dmitry B. Staroverov ◽  
Sergei Lukyanov ◽  
Vsevolod V. Belousov
Keyword(s):  
Hydrogen Peroxide ◽  
Hela Cells ◽  
Hydrogen Peroxide Production ◽  
Intracellular Hydrogen Peroxide ◽  
Stimulation Of

Induction of selenium-glutathione peroxidase by stimulation of metabolic hydrogen peroxide production in vivo

1987 ◽  
Vol 18 (1-3) ◽  
pp. 247-256 ◽  
Author(s):  
Jean Chaudiere ◽  
Dominique Gerard ◽  
Michel Clement ◽  
Jean-Marie Bourre
Keyword(s):  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Hydrogen Peroxide Production ◽  
Stimulation Of

scholarly journals Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS and/or Interleukins

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Lluvia Arteaga Figueroa ◽  
Rodolfo Abarca-Vargas ◽  
Claudia García Alanis ◽  
Vera L. Petricevich
Keyword(s):  
Hydrogen Peroxide ◽  
Cytotoxic Effect ◽  
Macrophage Activation ◽  
Peritoneal Macrophages ◽  
Immunomodulatory Activity ◽  
Hydrogen Peroxide Production ◽  
Griess Reagent ◽  
Violet Crystal ◽  
Low Cytotoxicity

Activation of macrophages may be one of the possible approaches in modulating inflammation. We previously reported that Bougainvillea xbuttiana extract showed an immunomodulatory activity. Here we compare the activation of macrophages exposed to B. xbuttiana extract and compare it with the other treatments such as LPS, IL-4, and IL-10. The cytotoxic effect of extract on peritoneal macrophages was determined by the technique of violet crystal staining. To verify the activation of macrophages we used the tests of vacuolization, hydrogen peroxide production, and percentages of cellular expansion and phagocytosis. The levels of interleukins secreted by macrophages treated with the extract, LPS, and cytokines were determined by the biological assay for the determination of TNF levels and by ELISA for all other interleukins. NO levels were evaluated by colorimetric reactions using Griess reagent. Our results showed that B. xbuttiana extract induced (a) low cytotoxicity percentages, (b) increased vacuolization, hydrogen peroxide production and cell expansion and phagocytosis percentages, and (c) decreased production of TNF-α, IFN-γ, IL-1β, and IL-6 and potentiated production of IL-4, IL-10 and TGF-β. These results suggest that B. xbuttiana extract was able to activate the murine macrophages in a manner similar to those macrophages exposed to IL-4 and IL-10.

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444 ◽  
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

Nitric Oxide, Superoxide, and Hydrogen Peroxide Production in Brain Mitochondria after Haloperidol Treatment

Nitric Oxide ◽  
1999 ◽  
Vol 3 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Silvia Lores Arnaiz ◽  
Marı́a Florencia Coronel ◽  
Alberto Boveris
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Brain Mitochondria ◽  
Hydrogen Peroxide Production ◽  
Haloperidol Treatment
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