Imaging of Intracellular Hydrogen Peroxide Production with HyPer upon Stimulation of HeLa Cells with EGF

2019 ◽  
pp. 85-91 ◽  
Author(s):  
Kseniya N. Markvicheva ◽  
Ekaterina A. Bogdanova ◽  
Dmitry B. Staroverov ◽  
Sergei Lukyanov ◽  
Vsevolod V. Belousov
Keyword(s):  
Hydrogen Peroxide ◽  
Hela Cells ◽  
Hydrogen Peroxide Production ◽  
Intracellular Hydrogen Peroxide ◽  
Stimulation Of

Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with Egf

2008 ◽  
pp. 76-83 ◽  
Author(s):  
Kseniya N. Markvicheva ◽  
Ekaterina A. Bogdanova ◽  
Dmitry B. Staroverov ◽  
Sergei Lukyanov ◽  
Vsevolod V. Belousov
Keyword(s):  
Hydrogen Peroxide ◽  
Hela Cells ◽  
Hydrogen Peroxide Production ◽  
Intracellular Hydrogen Peroxide ◽  
Stimulation Of

Induction of selenium-glutathione peroxidase by stimulation of metabolic hydrogen peroxide production in vivo

1987 ◽  
Vol 18 (1-3) ◽  
pp. 247-256 ◽  
Author(s):  
Jean Chaudiere ◽  
Dominique Gerard ◽  
Michel Clement ◽  
Jean-Marie Bourre
Keyword(s):  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Hydrogen Peroxide Production ◽  
Stimulation Of

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444 ◽  
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

scholarly journals Lichen Metabolites Modulate Hydrogen Peroxide and Nitric Oxide in Mouse Macrophages

2009 ◽  
Vol 64 (9-10) ◽  
pp. 664-672 ◽  
Author(s):  
Iracilda Z. Carlos ◽  
Marcela B. Quilles ◽  
Camila B. A. Carli ◽  
Danielle C. G. Maia ◽  
Fernanda P. Benzatti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Phenol Red ◽  
Hydrogen Peroxide Production ◽  
Griess Reagent ◽  
Mouse Macrophages ◽  
Lichen Metabolites ◽  
Oxidation Of Phenol ◽  
Peritoneal Macrophage Cells ◽  
Stimulation Of

The activities of perlatolic acid (1), atranorin (2), and lecanoric acid (3) and their derivatives, such as orsellinates and β-methyl orsellinates obtained by alcoholysis, were assessed for stimulation of the release of hydrogen peroxide and nitric oxide in cultures of peritoneal macrophage cells from mice. The hydrogen peroxide production was estimated by oxidation of phenol red, while the Griess reagent was used to determine the nitric oxide production. 1 and 4-methoxy-ethyl orsellinate (XVII) were the compounds that induced the greatest release of H2O2, whereas n-pentyl orsellinate (IV), iso-propyl orsellinate (V), sec-butyl orsellinate (VI), and XVII induced a small release of NO. These results indicate that lichen products and their derivatives have potential immune-modulating activities

scholarly journals Lactate dehydrogenases amplify reactive oxygen species in cancer cells in response to oxidative stimuli

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Hao Wu ◽  
Yuqi Wang ◽  
Minfeng Ying ◽  
Chengmeng Jin ◽  
Jiangtao Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Hela Cells ◽  
Hydrogen Peroxide Production ◽  
Physiological Relevance ◽  
4T1 Cells ◽  
Lactate Dehydrogenases ◽  
Transfer Chain

AbstractPrevious studies demonstrated that superoxide could initiate and amplify LDH-catalyzed hydrogen peroxide production in aqueous phase, but its physiological relevance is unknown. Here we showed that LDHA and LDHB both exhibited hydrogen peroxide-producing activity, which was significantly enhanced by the superoxide generated from the isolated mitochondria from HeLa cells and patients’ cholangiocarcinoma specimen. After LDHA or LDHB were knocked out, hydrogen peroxide produced by Hela or 4T1 cancer cells were significantly reduced. Re-expression of LDHA in LDHA-knockout HeLa cells partially restored hydrogen peroxide production. In HeLa and 4T1 cells, LDHA or LDHB knockout or LDH inhibitor FX11 significantly decreased ROS induction by modulators of the mitochondrial electron transfer chain (antimycin, oligomycin, rotenone), hypoxia, and pharmacological ROS inducers piperlogumine (PL) and phenethyl isothiocyanate (PEITC). Moreover, the tumors formed by LDHA or LDHB knockout HeLa or 4T1 cells exhibited a significantly less oxidative state than those formed by control cells. Collectively, we provide a mechanistic understanding of a link between LDH and cellular hydrogen peroxide production or oxidative stress in cancer cells in vitro and in vivo.

scholarly journals Stimulation of hydrogen peroxide production by drinking water contaminants in HL-60 cells sensitized by retinoic acid

1998 ◽  
Vol 3 (2) ◽  
pp. 106-112 ◽  
Author(s):  
Hideaki Yoshida ◽  
Shoichi Inoue ◽  
Kohki Yoshida ◽  
Osamu Nakajima ◽  
Shigetaka Mizuno
Keyword(s):  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Retinoic Acid ◽  
Hydrogen Peroxide Production ◽  
Water Contaminants ◽  
Stimulation Of
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