Two methods of transplanting single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis are described, illustrated, and tested.
The detached-cell method is simpler and quicker to operate and is suitable for homogeneous populations of cells which are easily removed from the substrate on which they are growing. The other, attached-cell, method is technically more elaborate, but is applicable to cells whose properties can be individually determined under the phase-contrast microscope and to cells which are not readily dissociated from other cells or from their substrate.
