scholarly journals A Shared Transcriptional Identity for Forebrain and Dentate Gyrus Neural Stem Cells from Embryogenesis to Adulthood

eNeuro ◽  
2022 ◽  
pp. ENEURO.0271-21.2021
Author(s):  
Michael J. Borrett ◽  
Nareh Tahmasian ◽  
Brendan T. Innes ◽  
Gary D. Bader ◽  
David R. Kaplan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dentate Gyrus

scholarly journals The Ventral Hippocampus Is the Embryonic Origin for Adult Neural Stem Cells in the Dentate Gyrus

Neuron ◽  
2013 ◽  
Vol 78 (4) ◽  
pp. 658-672 ◽  
Author(s):  
Guangnan Li ◽  
Li Fang ◽  
Gloria Fernández ◽  
Samuel J. Pleasure
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dentate Gyrus ◽  
Ventral Hippocampus ◽  
Adult Neural Stem Cells ◽  
Embryonic Origin

scholarly journals Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus

2012 ◽  
Vol 31 (2) ◽  
pp. 307-314 ◽  
Author(s):  
YUKI AKAZAWA ◽  
TAKAMASA KITAMURA ◽  
YURI FUJIHARA ◽  
YOSHITAKA YOSHIMURA ◽  
MASATO MITOME ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dentate Gyrus ◽  
Hippocampal Dentate Gyrus ◽  
Adult Neural Stem Cells

scholarly journals Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Branden R Nelson ◽  
Rebecca D Hodge ◽  
Ray AM Daza ◽  
Prem Prakash Tripathi ◽  
Sebastian J Arnold ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dentate Gyrus ◽  
Lineage Tracing ◽  
Cell Contact ◽  
Genetic Lineage ◽  
Multiphoton Imaging ◽  
Intermediate Progenitors ◽  
Dynamic Cell ◽  
Cell Cell

The hippocampal dentate gyrus (DG) is a unique brain region maintaining neural stem cells (NCSs) and neurogenesis into adulthood. We used multiphoton imaging to visualize genetically defined progenitor subpopulations in live slices across key stages of mouse DG development, testing decades old static models of DG formation with molecular identification, genetic-lineage tracing, and mutant analyses. We found novel progenitor migrations, timings, dynamic cell-cell interactions, signaling activities, and routes underlie mosaic DG formation. Intermediate progenitors (IPs, Tbr2+) pioneered migrations, supporting and guiding later emigrating NSCs (Sox9+) through multiple transient zones prior to converging at the nascent outer adult niche in a dynamic settling process, generating all prenatal and postnatal granule neurons in defined spatiotemporal order. IPs (Dll1+) extensively targeted contacts to mitotic NSCs (Notch active), revealing a substrate for cell-cell contact support during migrations, a developmental feature maintained in adults. Mouse DG formation shares conserved features of human neocortical expansion.

scholarly journals Isolation of the side population from adult neurogenic niches enriches for endothelial cells

2021 ◽  
Author(s):  
Alena Kalinina ◽  
Yingben Xue ◽  
Catherine Gnyra ◽  
Diane Lagace
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Dna Binding ◽  
Neural Stem Cells ◽  
Dentate Gyrus ◽  
Abc Transporter ◽  
Lateral Ventricle ◽  
Side Population ◽  
Adult Brain ◽  
Adult Neural Stem Cells

In stem cell research, DNA-binding dyes offer the ability to purify live stem cells using flow cytometry as they form a low-fluorescence side population due to the activity of ABC transporters. Adult neural stem cells exist within the lateral ventricle and dentate gyrus of the adult brain yet the ability of DNA-binding dyes to identify these adult stem cells as side populations remain untested. The following experiments utilize the efflux of a DNA-binding dye, Vyrbant DyeCycle Violet (DCV), to isolate bona fide side populations in the adult mouse dentate gyrus and SVZ and test their sensitivity to ABC transporter inhibitors. A distinct side population was found in both the adult lateral ventricle and dentate gyrus using DCV fluorescence and forward scatter instead of the conventional dual fluorescence approach. These side populations responded strongly to inhibition with the ABC transporter antagonists, verapamil and fumitremorgin C. The cells in the side population were identified as cerebrovascular endothelial cells characterized by their expression of CD31. These findings, therefore, suggest that the side population analysis provides an efficient method to purify endothelial cells, but not adult neural stem cells.

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