scholarly journals Differential expression of mouse neural cell-adhesion molecule (N-CAM) mRNA species during brain development and in neural cell lines

1986 ◽  
Vol 6 (7) ◽  
pp. 1983-1990 ◽  
Author(s):  
G Gennarini ◽  
MR Hirsch ◽  
HT He ◽  
M Hirn ◽  
J Finne ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Brain Development ◽  
Differential Expression ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Neural Cell Adhesion ◽  
Neural Cell Lines

scholarly journals Neural Cell Adhesion Molecule Contributes to Hemopoiesis-Supporting Capacity of Stromal Cell Lines

Stem Cells ◽  
2005 ◽  
Vol 23 (9) ◽  
pp. 1389-1399 ◽  
Author(s):  
Xiaoli Wang ◽  
Hiroko Hisha ◽  
Shigeru Taketani ◽  
Muneo Inaba ◽  
Qiang Li ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Stromal Cell ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Supporting Capacity ◽  
Neural Cell Adhesion

scholarly journals Skeletal muscle neural cell adhesion molecule (N-CAM): changes in protein and mRNA species during myogenesis of muscle cell lines.

1987 ◽  
Vol 105 (3) ◽  
pp. 1377-1386 ◽  
Author(s):  
S E Moore ◽  
J Thompson ◽  
V Kirkness ◽  
J G Dickson ◽  
F S Walsh
Keyword(s):  
Cell Adhesion ◽  
Phospholipase C ◽  
Cell Lines ◽  
Muscle Cell ◽  
Adhesion Molecule ◽  
Indirect Immunofluorescence ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Neural Cell Adhesion

Qualitative and quantitative changes in neural cell adhesion molecule (N-CAM) protein and mRNA forms were measured during myogenesis in G8-1 and C2 cell lines. Indirect immunofluorescence assay showed that N-CAM was constitutively expressed by myoblasts in culture and that myotubes appeared to be stained more strongly. These changes were quantified using a dot blot assay. N-CAM levels increased almost 4-fold in G8-1 cells and 15-fold in C2 cells during myogenesis. The kinetics of accumulation of N-CAM were not coordinate with other muscle markers such as creatine kinase or acetylcholine receptor levels, since N-CAM accumulated significantly ahead of these markers. Immunoblotting showed that myogenesis was not associated with changes in the extent of sialylation of N-CAM. However, distinct changes in desialo forms were observed after neuraminidase treatment. Myogenesis was accompanied by increases in 125- and 155-kD desialo forms with minor changes in 120- and 145-kD forms. Biosynthetic labeling studies showed that myoblasts specifically expressed a transmembrane isoform of 145 kD that was phosphorylated and was down-regulated in myotubes. Pulse-chase analysis of myotubes showed that the 120-kD isoform and an isoform of 145 kD that co-migrated with, but was distinct from, the 145 kD transmembrane isoform of myoblasts were precursors of the 125- and 155-kD isoforms, respectively, that accumulated in myotubes. The 125- and 155-kD isoforms in myotubes are linked to the cell membrane via phosphatidylinositol linkage and can be released by phospholipase C. Indirect immunofluorescence analysis showed that phosphatidylinositol specific phospholipase C specifically released N-CAM from the myotube membrane generating N-CAM-free myotubes, while myoblasts were unaffected by this treatment. Three N-CAM mRNA species were observed in mouse muscle cell lines. Myoblasts were characterized by their expression of 6.7- and 5.2-kb transcripts while myotubes express 5.2- and 2.9-kb transcripts. Thus, myogenesis is qualitatively associated with a down regulation of the 6.7-kb transcript and an up regulation of the 5.2- and 2.9-kb transcript.

scholarly journals Differential expression of the neural cell adhesion molecule NCAM 140 in human pituitary tumors

FEBS Letters ◽  
1990 ◽  
Vol 272 (1-2) ◽  
pp. 45-49 ◽  
Author(s):  
Maria C. Aletsee-Ufrecht ◽  
Keith Langley ◽  
Otmar Gratzl ◽  
Manfred Gratzl
Keyword(s):  
Cell Adhesion ◽  
Differential Expression ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Pituitary Tumors ◽  
Neural Cell ◽  
Human Pituitary ◽  
Neural Cell Adhesion

scholarly journals Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.

1989 ◽  
Vol 169 (6) ◽  
pp. 2233-2238 ◽  
Author(s):  
L L Lanier ◽  
R Testi ◽  
J Bindl ◽  
J H Phillips
Keyword(s):  
Cell Adhesion ◽  
Nk Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neuroblastoma Cell ◽  
Neural Cell ◽  
Neural Cell Adhesion

Neural cell adhesion molecule (N-CAM) is a membrane glycoprotein expressed on neural and muscle tissues that is involved in homotypic adhesive interactions. We have demonstrated that N-CAM also is expressed on hematopoietic cells, and is recognized by the anti-Leu-19 mAb. Leu-19 is preferentially expressed on NK cells and T lymphocytes that mediate MHC-unrestricted cytotoxicity, but is also present on some myeloid leukemia cell lines. On NK cells, T cells, the KG1a.5 hematopoietic cell line, and a neuroblastoma cell line, Leu-19 is a approximately 140-kD polypeptide with N-linked carbohydrates and abundant sialic acid residues. Sequential immunoprecipitation and peptide mapping demonstrated that the Leu-19 and N-CAM molecules expressed on leukocyte and neuroblastoma cell lines are similar structures. These findings suggest that the Leu-19 antigen on leukocytes may be involved in cell adhesion, analogous to the function on N-CAM on neural cells.

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