scholarly journals Sodium Channels Contribute to Degeneration of Dorsal Root Ganglion Neurites Induced by Mitochondrial Dysfunction in an In Vitro Model of Axonal Injury

2013 ◽  
Vol 33 (49) ◽  
pp. 19250-19261 ◽  
Author(s):  
A.-K. Persson ◽  
I. Kim ◽  
P. Zhao ◽  
M. Estacion ◽  
J. A. Black ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Mitochondrial Dysfunction ◽  
Sodium Channels ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Axonal Injury ◽  
Vitro Model

scholarly journals Dorsal Root Ganglion Neurons Carrying a P301S Tau Mutation: A Valid In Vitro Model for Screening Drugs against Tauopathies?

2014 ◽  
Vol 34 (14) ◽  
pp. 4757-4759 ◽  
Author(s):  
Niels Alberts ◽  
Karlijn Groen ◽  
Lisette Klein ◽  
Marek J. Konieczny ◽  
Mandy Koopman
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Dorsal Root Ganglion Neurons ◽  
Vitro Model ◽  
Ganglion Neurons ◽  
Tau Mutation

scholarly journals Differentiated neuroblastoma F-11 cells as an alternative in-vitro model to dorsal root ganglion neurons

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Stefania Blasa ◽  
Valentina Pastori ◽  
Alessia D’Aloia ◽  
Marzia M. Lecchi
Keyword(s):  
Animal Models ◽  
Dorsal Root Ganglion ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Dorsal Root Ganglion Neurons ◽  
Biomedical Field ◽  
Vitro Model ◽  
Ganglion Neurons

We induced differentiation in F-11 cells to verify if they could show similarities with sensory neurons, in order to develop an alternative to animal models for research studies in the biomedical field.

In vitro characterization of a peripheral afferent pathway of the rat after chronic sciatic nerve section

1996 ◽  
Vol 76 (5) ◽  
pp. 3169-3177 ◽  
Author(s):  
R. C. Babbedge ◽  
A. J. Soper ◽  
C. T. Gentry ◽  
V. C. Hood ◽  
E. A. Campbell ◽  
...  
Keyword(s):  
Peripheral Neuropathy ◽  
Sciatic Nerve ◽  
Dorsal Root Ganglion ◽  
Peripheral Nerve ◽  
Spontaneous Activity ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Alpha Beta ◽  
Vitro Model

1. We have studied the characteristics of the abnormal properties of damaged myelinated fibers (conduction velocity > 2.0 m/ s) after peripheral nerve injury in a novel in vitro model of the rat sciatic nerve/dorsal root ganglion/dorsal root (L4-5) preparation removed from control naive or sham-operated rats and animals that had received sciatic neurectomy 12-24 days before the in vitro study. A total of 122-245 filaments were recorded in each dorsal root. The proportion of A alpha, beta and A delta fibers were not significantly different between control, sham-operated, and axotomized nerves. Spontaneous activity was recorded in 3.4% (A alpha, beta) and 4.6% (A delta) of fibers in comparison with 0.4% (A alpha, beta) and 0.3% (A delta) in naive controls. 2. A sporadic, irregular, low-frequency (< 1 Hz) firing was seen in 26% of the fibers with spontaneous activity. Periodical (irregular) bursting pattern was observed in 43% of spontaneously active fibers, whereas a relatively stable, ongoing firing pattern (median frequency: 7.1 Hz) was displayed by 31% of active fibers. 3. Mechanosensitivity of the neuroma/peripheral nerve was displayed in preparations from lesioned [axotomized: 18.2% (A alpha, beta) and 14.1% (A delta), sham operated: 2% (A alpha, beta) and 0% (A delta)], but not control naive animals. There was no correlation between the presence of spontaneous activity and mechanosensitivity in single fibers. 4. The principal site of spontaneous activity generation was the dorsal root ganglion. Transection of the peripheral nerve (or removal of the neuroma), while recording from dorsal root filaments, produced a cessation of firing in 21% of fibers firing with ongoing discharge. The remaining active fibers continued firing until the DRG was removed. A sustained injury discharge was observed in damaged fibers but not control, undamaged fibers from naive animals after acute peripheral nerve transection. 5. We present an in vitro model for the study of abnormal primary sensory activity in peripheral neuropathy. Although our data are consistent with in vivo electrophysiological findings in published reports, the proportion of damaged afferent fibers displaying spontaneous activity was significantly lower under in vitro conditions. This model may serve as a valuable tool for further physiological and pharmacological studies of peripheral neuropathy.

scholarly journals In Vitro Model to Investigate Communication between Dorsal Root Ganglion and Spinal Cord Glia

2021 ◽  
Vol 22 (18) ◽  
pp. 9725
Author(s):  
Junxuan Ma ◽  
Vaibhav Patil ◽  
Abhay Pandit ◽  
Leo R. Quinlan ◽  
David P. Finn ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Root Ganglion ◽  
Conditioned Medium ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Neonatal Rat ◽  
Large Animal ◽  
Large Species ◽  
Vitro Model

Chronic discogenic back pain is associated with increased inflammatory cytokine levels that can influence the proximal peripheral nervous system, namely the dorsal root ganglion (DRG). However, transition to chronic pain is widely thought to involve glial activation in the spinal cord. In this study, an in vitro model was used to evaluate the communication between DRG and spinal cord glia. Primary neonatal rat DRG cells were treated with/without inflammatory cytokines (TNF-α, IL-1β, and IL-6). The conditioned media were collected at two time points (12 and 24 h) and applied to spinal cord mixed glial culture (MGC) for 24 h. Adult bovine DRG and spinal cord cell cultures were also tested, as an alternative large animal model, and results were compared with the neonatal rat findings. Compared with untreated DRG-conditioned medium, the second cytokine-treated DRG-conditioned medium (following medium change, thus containing solely DRG-derived molecules) elevated CD11b expression and calcium signal in neonatal rat microglia and enhanced Iba1 expression in adult bovine microglia. Cytokine treatment induced a DRG-mediated microgliosis. The described in vitro model allows the use of cells from large species and may represent an alternative to animal pain models (3R principles).

Dorsal root ganglia cocultured with macrophages: an in vitro model to study experimental demyelination

1994 ◽  
Vol 88 (5) ◽  
pp. 459-464 ◽  
Author(s):  
W. Br�ck ◽  
Y. Br�ck ◽  
U. Diederich ◽  
R. L. Friede
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Experimental Demyelination ◽  
Vitro Model

Dorsal Root Ganglia as an in Vitro Model for Melanocortin-Induced Neuritogenesis..

1994 ◽  
Vol 739 (1 Models of Neu) ◽  
pp. 74-86 ◽  
Author(s):  
E. M. HOL ◽  
P. SODAAR ◽  
P. R. BÄR
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Vitro Model

Cellular Reactivity to Mechanical Axonal Injury in an Organotypic in Vitro Model of Neurotrauma

1999 ◽  
Vol 16 (12) ◽  
pp. 1197-1213 ◽  
Author(s):  
FRANK SIEG ◽  
PETRA WAHLE ◽  
HANS-CHRISTIAN PAPE
Keyword(s):  
In Vitro Model ◽  
Axonal Injury ◽  
Vitro Model
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