scholarly journals A Hands-On Set for Understanding DNA Replication, Transcription & Polymerase Chain Reaction (PCR)

2020 ◽  
Vol 82 (1) ◽  
pp. 49-51
Author(s):  
Ladislav Merta ◽  
Tomáš Pinkr ◽  
Vanda Janštová
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Replication ◽  
Molecular Biology ◽  
Chain Reaction ◽  
Hands On ◽  
Polymerase Chain

Molecular biology topics tend to be abstract and hard to visualize, and consequently pupils form many misconceptions about genetics and molecular biology. We describe how to make a hands-on educational set that provides visual and tactile modeling of DNA replication, transcription, polymerase chain reaction (PCR), and random mutations so that students can examine these processes in detail. The set is inexpensive and easy to make, has been used successfully, and allows for modification to fit individual teachers' needs.

An intensive hands-on course designed to teach molecular biology techniques to physiology graduate students

2002 ◽  
Vol 26 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Andrea D. Weston ◽  
Sasha Stasko ◽  
Gerald M. Kidder
Keyword(s):  
Polymerase Chain Reaction ◽  
Graduate Students ◽  
Molecular Biology ◽  
Historical Perspective ◽  
Rna Expression ◽  
Chain Reaction ◽  
Hands On ◽  
Similar Information ◽  
Polymerase Chain ◽  
Intensive Course

To address a growing need to make research trainees in physiology comfortable with the tools of molecular biology, we have developed a laboratory-intensive course designed for graduate students. This course is offered to a small group of students over a three-week period and is organized such that comprehensive background lectures are coupled with extensive hands-on experience. The course is divided into seven modules, each organized by a faculty member who has particular expertise in the area covered by that module. The modules focus on basic methods such as cDNA subcloning, sequencing, gene transfer, polymerase chain reaction, and protein and RNA expression analysis. Each module begins with a lecture that introduces the technique in detail by providing a historical perspective, describing both the uses and limitations of that technique, and comparing the method with others that yield similar information. Most of the lectures are followed by a laboratory session during which students follow protocols that were carefully designed to avoid pitfalls. Throughout these laboratory sessions, students are given an appreciation of the importance of proper technique and accuracy. Communication among the students, faculty, and the assistant coordinator is focused on when and why each procedure would be used, the importance of each step in the procedure, and approaches to troubleshooting. The course ends with an exam that is designed to test the students’ general understanding of each module and their ability to apply the various techniques to physiological questions.

Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip

RSC Advances ◽  
2015 ◽  
Vol 5 (16) ◽  
pp. 12071-12077 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Yu Zhang ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Flow Rate ◽  
Chain Reaction ◽  
Long Distance ◽  
Chip Flow ◽  
Hands On ◽  
Plastic Syringe ◽  
Flow Through ◽  
Polymerase Chain ◽  
On Chip

A strategy for realizing self-actuated pumping with uniform flow rate over a long distance is introduced using hands-on operation of disposable syringe, and was applied for on-chip flow-through PCR inside a serpentine PMMA microchannel.

Molecular biology made easy. The polymerase chain reaction

1992 ◽  
Vol 24 (12) ◽  
pp. 913-926 ◽  
Author(s):  
A. M. T. Clarke ◽  
N. P. Mapstone ◽  
P. Quirke
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Chain Reaction ◽  
Polymerase Chain

An initiation zone of chromosomal DNA replication located upstream of the c-myc gene in proliferating HeLa cells

1990 ◽  
Vol 10 (9) ◽  
pp. 4899-4904
Author(s):  
L Vassilev ◽  
E M Johnson
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Replication ◽  
Hela Cells ◽  
Polymerase Chain Reaction Amplification ◽  
Chromosomal Dna ◽  
Chain Reaction ◽  
Initiation Zone ◽  
Myc Gene ◽  
Reaction Amplification ◽  
Polymerase Chain

Studies on origins of DNA replication in mammalian cells have long been hampered by a lack of methods sensitive enough for the localization of such origins in chromosomal DNA. We have employed a new method for mapping origins, based on polymerase chain reaction amplification of nascent strand segments, to examine replication initiated in vivo near the c-myc gene in human cells. Nascent DNA, pulse-labeled in unsynchronized HeLa cells, was size fractionated and purified by immunoprecipitation with anti-bromodeoxyuridine antibodies. Lengths of the nascent strands that allow polymerase chain reaction amplification were determined by hybridization to probes homologous to amplified segments and used to calculate the position of the origin. We found that DNA replication through the c-myc gene initiates in a zone centered approximately 1.5 kilobases upstream of exon I. Replication proceeds bidirectionally from the origin, as indicated by comparison of hybridization patterns for three amplified segments. The initiation zone includes segments of the c-myc locus previously reported to drive autonomous replication of plasmids in human cells.

scholarly journals ОГЛЯД МЕТОДІВ ТА МОДЕЛЕЙ ПОЛІМЕРАЗНО-ЛАНЦЮГОВОЇ РЕАКЦІЇ

2015 ◽  
Author(s):  
A. S. Sverstyuk ◽  
T. V. Bihunyak ◽  
B. O. Pereviznyk
Keyword(s):  
Mathematical Modeling ◽  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Minimum Time ◽  
Chain Reaction ◽  
Reaction Models ◽  
Polymerase Chain ◽  
Time Required

In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques efficiency.

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