Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip

RSC Advances ◽  
2015 ◽  
Vol 5 (16) ◽  
pp. 12071-12077 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Yu Zhang ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Flow Rate ◽  
Chain Reaction ◽  
Long Distance ◽  
Chip Flow ◽  
Hands On ◽  
Plastic Syringe ◽  
Flow Through ◽  
Polymerase Chain ◽  
On Chip

A strategy for realizing self-actuated pumping with uniform flow rate over a long distance is introduced using hands-on operation of disposable syringe, and was applied for on-chip flow-through PCR inside a serpentine PMMA microchannel.

On-chip polymerase chain reaction microdevice employing a magnetic droplet-manipulation system

2008 ◽  
Vol 130 (2) ◽  
pp. 583-588 ◽  
Author(s):  
Hiroyoshi Tsuchiya ◽  
Mina Okochi ◽  
Nobuhiro Nagao ◽  
Mitsuhiro Shikida ◽  
Hiroyuki Honda
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Manipulation System ◽  
Polymerase Chain ◽  
Droplet Manipulation ◽  
On Chip ◽  
Magnetic Droplet

An intensive hands-on course designed to teach molecular biology techniques to physiology graduate students

2002 ◽  
Vol 26 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Andrea D. Weston ◽  
Sasha Stasko ◽  
Gerald M. Kidder
Keyword(s):  
Polymerase Chain Reaction ◽  
Graduate Students ◽  
Molecular Biology ◽  
Historical Perspective ◽  
Rna Expression ◽  
Chain Reaction ◽  
Hands On ◽  
Similar Information ◽  
Polymerase Chain ◽  
Intensive Course

To address a growing need to make research trainees in physiology comfortable with the tools of molecular biology, we have developed a laboratory-intensive course designed for graduate students. This course is offered to a small group of students over a three-week period and is organized such that comprehensive background lectures are coupled with extensive hands-on experience. The course is divided into seven modules, each organized by a faculty member who has particular expertise in the area covered by that module. The modules focus on basic methods such as cDNA subcloning, sequencing, gene transfer, polymerase chain reaction, and protein and RNA expression analysis. Each module begins with a lecture that introduces the technique in detail by providing a historical perspective, describing both the uses and limitations of that technique, and comparing the method with others that yield similar information. Most of the lectures are followed by a laboratory session during which students follow protocols that were carefully designed to avoid pitfalls. Throughout these laboratory sessions, students are given an appreciation of the importance of proper technique and accuracy. Communication among the students, faculty, and the assistant coordinator is focused on when and why each procedure would be used, the importance of each step in the procedure, and approaches to troubleshooting. The course ends with an exam that is designed to test the students’ general understanding of each module and their ability to apply the various techniques to physiological questions.

A Reusable Flow-Through Polymerase Chain Reaction Instrument for the Continuous Monitoring of Infectious Biological Agents

2003 ◽  
Vol 75 (14) ◽  
pp. 3446-3450 ◽  
Author(s):  
Phillip Belgrader ◽  
Christopher J. Elkin ◽  
Steven B. Brown ◽  
Shanavaz N. Nasarabadi ◽  
Richard G. Langlois ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Continuous Monitoring ◽  
Biological Agents ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Instrument ◽  
Flow Through ◽  
Polymerase Chain

Renewable microcolumns for automated DNA purification and flow-through amplification: from sediment samples through polymerase chain reaction

2002 ◽  
Vol 469 (1) ◽  
pp. 129-140 ◽  
Author(s):  
Cynthia J. Bruckner-Lea ◽  
Toyoko Tsukuda ◽  
Brian Dockendorff ◽  
James C. Follansbee ◽  
Mark T. Kingsley ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Purification ◽  
Chain Reaction ◽  
Sediment Samples ◽  
Flow Through ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document